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Table 3

Effect of TRP-2 Mutations within TRP-2181–188 on Recognition by Line A T Cells when Transfected into 293 Cells Expressing Murine MHC Class I Molecules

IFN-γ Release
Transfected cDNATransfection targets
293KbDb293Kb293
    U/ml*   
Clone 3.6  17.3  10.8  1.9 
Clone 3.6 mut 185   1.0   1.2  2.0 
Clone 3.6 mut185 +188§   1.0   1.5  2.2 
pcDNA3   0.7   1.3  2.0 
IFN-γ Release
Transfected cDNATransfection targets
293KbDb293Kb293
    U/ml*   
Clone 3.6  17.3  10.8  1.9 
Clone 3.6 mut 185   1.0   1.2  2.0 
Clone 3.6 mut185 +188§   1.0   1.5  2.2 
pcDNA3   0.7   1.3  2.0 
*

 Line A T cells were incubated with the indicated targets for 24 h and the release of IFN-γ measured.  

 Amino acid185 phenylalanine substituted with aspartate.  

§

   Amino acid185 phenylalanine substituted with aspartate, and amino acid188 leucine substituted with arginine.  

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