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Table 2

Determination of MHC Restriction by Transfection of TRP-2 cDNA Clones into 293 Cells Expressing Different MHC Molecules

IFN-γ Release
Transfected cDNATransfection targets
293KbDb293Kb293-A2
  U/ml* 
Clone 5.3  7.0  10.7  0.5 
Clone 6.4  9.3  10.5  0.5 
Clone 7.8  8.1   7.3  0.5 
None  0.6   0.6  0.5 
IFN-γ Release
Transfected cDNATransfection targets
293KbDb293Kb293-A2
  U/ml* 
Clone 5.3  7.0  10.7  0.5 
Clone 6.4  9.3  10.5  0.5 
Clone 7.8  8.1   7.3  0.5 
None  0.6   0.6  0.5 
*

 Line A T cells were incubated with the indicated targets for 24 h and the release of IFN-γ measured.  

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