Figure 1. Persistent coupling of α-cat to F-actin enhances binucleation rate. (A) Schematic of α-cat domains based on crystal structure. α-cat-H0-ABD⁺ replaces four amino acids (RAIM>GSGS) in the first kinked helix of α-cat’s five-helical More about this image found in Persistent coupling of α-cat to F-actin enhances binucleation rate. (A) Sc...

Figure 2. Persistent coupling of α-cat to F-actin promotes binucleation via cytokinesis failure. (A) Live imaging analysis of MDCK α-cat KO cells restored with α-cat-WT(Top) or α-cat-H0-ABD⁺(Bottom) during mitosis phases (metaphase, anaphase, More about this image found in Persistent coupling of α-cat to F-actin promotes binucleation via cytokines...

Figure 3. α-cat-H0-ABD ⁺ -induced cytokinesis failure requires the mechanosensitive M-domain. (A) Schematic of α-cat mutants used for analysis. More (+++) or less (+) F-actin binding is predicted from previous in-solution actin More about this image found in α-cat-H0-ABD ⁺ -induced cytokinesis failure requires the mecha...

Figure 4. α-cat M-domain missense mutants that cause eye dystrophy promote wound-proximal binucleation. (A) Schematic of α-cat missense mutants used for analysis. (B) Immunoblots of total cell extracts showing similar expression levels of More about this image found in α-cat M-domain missense mutants that cause eye dystrophy promote wound-prox...

Figure 5. α-cat proximity partner, LZTS2, localizes to apical junctions, base of primary cilia and midbody and is required for successful cell division in MDCK cells. (A) Confocal en face image (maximum z-projection; inverted grayscale) and More about this image found in α-cat proximity partner, LZTS2, localizes to apical junctions, base of prim...

Figure 6. α-cat M-domain butterfly-patterned eye dystrophy missense mutants show wound-front enhanced junctional recruitment of LZTS2. (A) Confocal en face views (maximum z-projection) of α-cat wild-type and mutant -expressing MDCK cells (GFP+) More about this image found in α-cat M-domain butterfly-patterned eye dystrophy missense mutants show woun...

Figure 7. α-cat-ΔM1 preferentially recruits LZTS2 to cell contacts over wild-type and other α-cat mutants. (A) Confocal en face views (maximum z-projection; inverted grayscale) and x-z stacks showing α-cat-ΔM1 enriches LZTS2 at cellular More about this image found in α-cat-ΔM1 preferentially recruits LZTS2 to cell contacts over wild-type and...

Figure 8. LZTS2 recruitment by α-cat ΔM1 is more uniform and refractory to myosin-inhibition compared with WT α-cat. (A and C) Coverslip grown MDCK cells treated with blebbistatin (100 μM, 3-h) reduced LZTS2 (magenta) localization to bicellular More about this image found in LZTS2 recruitment by α-cat ΔM1 is more uniform and refractory to myosin-inh...

Figure 9. LZTS2 enriches at junctions proximal to the apically resolving intercellular bridge during abscission. (A) Confocal image of intercellular bridge (acetylated tubulin, cyan) flanked by LZTS2 enrichment (magenta) in α-cat-WT restored More about this image found in LZTS2 enriches at junctions proximal to the apically resolving intercellula...

Figure 10. α-cat-ΔM1 monolayers show enhanced binucleation rate over wild-type and other α-cat mutants. (A) Confocal en face view (maximum z-projection; grayscale inverted) images showing GFP-α-catΔM1 expressing cells exhibit polyploidy after 12 More about this image found in α-cat-ΔM1 monolayers show enhanced binucleation rate over wild-type and oth...

Figure 1. FCS-calibrated imaging of Cohesin isoforms shows that reorganization of loop extrusion during mitotic exit takes about 2 h after anaphase onset. (A) Schematic of current loop-extrusion–based models of mitotic and interphase genome More about this image found in FCS-calibrated imaging of Cohesin isoforms shows that reorganization of loo...

Figure 2. Functional nuclear pores are required for nuclear import of Cohesin and CTCF. (A) Experiment scheme for mitotic synchronization of genome-edited HK cells with homozygously mEGFP-FKBP12^F36V tagged Nup153, followed by targeted protein More about this image found in Functional nuclear pores are required for nuclear import of Cohesin and CTC...

Figure 3. Condensins and Cohesins co-occupy chromatin during telophase and early G1, as revealed by time-resolved bleaching. (A) Illustration of the spot-bleach assay. Genome-edited HK cells homozygously expressing (m) EGFP-tagged Condensin and More about this image found in Condensins and Cohesins co-occupy chromatin during telophase and early G1, ...

Figure 4. Cohesin-STAG1 and CTCF cooperate to form interphase TAD structures after mitosis. (A) FCS-calibrated protein numbers colocalizing with chromatin displayed for genome-edited HK cells with homozygously EGFP-tagged Cohesin-STAG1 (n = 25 More about this image found in Cohesin-STAG1 and CTCF cooperate to form interphase TAD structures after mi...