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ASIC membrane-spanning region.  (A)  Left, MD simulation system for rASIC2a...
Published: 17 January 2020
Figure 1. ASIC membrane-spanning region. (A) Left, MD simulation system for rASIC2a model based on PDB accession no. 4NTW (each subunit a different color) embedded in a lipid bilayer (cyan) in NaCl solution (water, red and white sticks; Na + and Cl − , orange and cyan balls). Right, magnifie... More
Images
Site-directed mutagenesis and relative ion permeability of homomeric ASIC1a...
Published: 17 January 2020
Figure 2. Site-directed mutagenesis and relative ion permeability of homomeric ASIC1a and ASIC2a. (A) Protocol and example recordings of current–voltage (I-V) relationship for WT rASIC2a and indicated mutants, assayed by proton-gated current during −80 → 60 mV ramp; current during voltage ramp i... More
Images
D  ifferences in the structures of cASIC1 and rASIC2a.  (A)  Pore size for ...
Published: 17 January 2020
Figure S1. D ifferences in the structures of cASIC1 and rASIC2a. (A) Pore size for cASIC1 (red) and equilibrated rASIC2a model (blue) calculated using HOLE ( Smart et al., 1996 ), based on the last 100 ns of each simulation, with error bars based on SEMs from five blocks. Positions of L7′, GAS... More
Images
Structural alignments of M2 helices. (A–F)  Structural alignments of M2 hel...
Published: 17 January 2020
Figure S2. Structural alignments of M2 helices. (A–F) Structural alignments of M2 helices between cASIC1 crystal structure (purple) and mASIC1a/mASIC1a/rASIC2a heteromers (blue) for Na + (left) and K + (right; A and B), mASIC1a/rASIC2a/rASIC2a heteromers (blue; C and D), and rASIC2a homomers (... More
Images
RMSD for the backbone of TM1 and TM2 (residues 45 to 75 and 425 to 456 in r...
Published: 17 January 2020
Figure S3. RMSD for the backbone of TM1 and TM2 (residues 45 to 75 and 425 to 456 in red, and residues 60 to 75 and 425 to 454 in blue; PDB accession no. 4NTW numbering). (A and B) mASIC1a/mASIC1a/rASIC2a heteromers with NaCl (A) and KCl (B). (C and D) mASIC1a/rASIC2a/rASIC2a heteromers ... More
Images
Behavior of the lower pore of cASIC1 and rASIC2a.  (A and B)  Distribution ...
Published: 17 January 2020
Figure S4. Behavior of the lower pore of cASIC1 and rASIC2a. (A and B) Distribution of single and double occupancy for sites formed by E18′ and D21′ for cASIC1 (A) and rASIC2a (B) channels (mean ± SEM). Single ion occupancy is preferred in cASIC1 and rASIC2a for both Na + and K + ; however, do... More
Images
PMFs for Na +  and K + .  (A–  F  )  PMFs for Na +  (orange) and K +  (blue...
Published: 17 January 2020
Figure S5. PMFs for Na + and K + . (A– F ) PMFs for Na + (orange) and K + (blue) permeating the pore of mASIC1a/mASIC1a/rASIC2a heteromers (A and D) from US simulations (A) and free simulations (D); mASIC1a/rASIC2a/rASIC2a heteromers (B and E) from US simulations (B) and free simulations (... More
Images
Convergence of PMFs from US simulation.  (A and D)  mASIC1a/mASIC1a/rASIC2a...
Published: 17 January 2020
Figure S6. Convergence of PMFs from US simulation. (A and D) mASIC1a/mASIC1a/rASIC2a heteromers with Na + (A) and K + (D). (B and E) mASIC1a/rACIS2a/rASIC2a heteromers with Na + (B) and K + (E). (C and F) rASIC2a with Na + (C) and K + (F). Above the dotted line at z = 11 Å, there were... More
Images
r  ASIC2a MD free energy simulations. (A)  Single ion free energy profiles ...
Published: 17 January 2020
Figure 3. r ASIC2a MD free energy simulations. (A) Single ion free energy profiles for Na + (orange) and K + (blue) through the pore of cASIC1 (data from rASIC2a model and Lynagh et al. [2017a] shown as solid and dotted lines, respectively), with error bars represented by ±1 SEM. Inset show... More
Images
Expression mASIC1a/rASIC2a heteromers. (A)  Example current responses (left...
Published: 17 January 2020
Figure 4. Expression mASIC1a/rASIC2a heteromers. (A) Example current responses (left) to decreasing pH in oocytes injected with cRNAs for mASIC1a; mASIC1a and rASIC2a (in a 1:1 ratio); or rASIC2a and averaged (±SEM), normalized peak current responses to decreasing pH (right panel, n = 4–6). 1a... More
Images
Behavior of the upper pore of cASIC1 and rASIC2a.  (A)  Distribution of sin...
Published: 17 January 2020
Figure S7. Behavior of the upper pore of cASIC1 and rASIC2a. (A) Distribution of single and double occupancy in rASIC2a in sites formed by E59, E62, and E0′ showing occupancy and number of carboxylates (mean ± SEM). Single-ion occupancy is preferred for both Na + and K + ; however, double occu... More
Images
First shell coordination by oxygen atoms from water and protein.  (A and B)...
Published: 17 January 2020
Figure S8. First shell coordination by oxygen atoms from water and protein. (A and B) cASIC1 pore for Na + (A) and K + (B). (C and D) rASIC2a pore for Na + (C) and K + (D). (E and F) mASIC1a/rASIC2a heteromer pore for (E) Na + and (F) K + . Values plotted are mean ± SEM. More
Images
Lack of functional expression when replacing A11′ with lactic acid (α) in r...
Published: 17 January 2020
Figure S9. Lack of functional expression when replacing A11′ with lactic acid (α) in rASIC2a channels. Previous work shows that coinjection of oocytes with mutant mASIC1a cRNA, containing the amber stop codon (UAG) instead of the A11′ codon, with hydroxyacylated Tetrahymena thermophila tRNA (t... More
Images
Effects of M2 mutations on ion selectivity and MD simulations with ASIC1a/A...
Published: 17 January 2020
Figure 5. Effects of M2 mutations on ion selectivity and MD simulations with ASIC1a/ASIC2a heteromers. (A) Examples of pH 5.0–gated current through WT and mutant heteromeric channels in single oocytes. (B) Relative permeability ratios ( P Na + / P X + ) and half-maximal pH fo... More
Images
Comparison of the PMFs for cASIC1, rASIC2a, and mASIC1a/rASIC2a heteromers....
Published: 17 January 2020
Figure S10. Comparison of the PMFs for cASIC1, rASIC2a, and mASIC1a/rASIC2a heteromers. mASIC1a/rASIC2a is the average of mASIC1a/mASIC1a/rASIC2a and mASIC1a/rASIC2a/rASIC2a to reflect experiments, with error bars represented by ±1 SEM. (A and B) Na + permeating the pore (A) and K + permeati... More
Journal Articles
The Journal of General Physiology (2020) 152 (2): e201812297.
Published: 17 January 2020
Journal Articles
The Journal of General Physiology (2020) 152 (2): e201912501.
Published: 14 January 2020
Includes: Supplementary data
Journal Articles
The Journal of General Physiology (2020) 152 (2): e201912557.
Published: 10 January 2020
Images
Briana Bohannon (left), Peter Larsson (center), and colleagues reveal that ...
Published: 10 January 2020
Briana Bohannon (left), Peter Larsson (center), and colleagues reveal that PUFAs with distinct head groups can differentially activate the cardiac I Ks channels that terminate ventricular action potentials, raising the prospect of personalized treatments for LQTS. The graph (right) shows that, comp... More
Journal Articles
The Journal of General Physiology (2019) 152 (1): e201912455.
Published: 24 December 2019
Includes: Supplementary data