1-20 of 27184
Follow your search
Access your saved searches in your account

Would you like to receive an alert when new items match your search?
Close Modal
Sort by
Journal Articles
The Journal of General Physiology (2020) 152 (6): e201912498.
Published: 24 January 2020
Images
Derivatization of WGA with a small-molecule fluorescent pH sensor and cell ...
Published: 24 January 2020
Figure 1. Derivatization of WGA with a small-molecule fluorescent pH sensor and cell surface labeling to visualize plasma membrane proton fluxes. (A) Synthetic scheme of WGA-pHRho. Using the degree of labeling equation (Materials and methods) on average, one WGA monomer is labeled with one pHRh... More
Images
WGA labeling and voltage-clamp fluorometry of CHO cells expressing voltage-...
Published: 24 January 2020
Figure 2. WGA labeling and voltage-clamp fluorometry of CHO cells expressing voltage-gated channels. (A) hHv-1 (top) and Shaker-IR (bottom) currents were recorded before (left) and after (right) WGA labeling (50 µg/ml). Middle panels are currents before (black) and after (red) WGA wash-in at 60 ... More
Images
Characterization of pH-sensitive WGA conjugates, related to    Fig. 2   .  ...
Published: 24 January 2020
Figure S1. Characterization of pH-sensitive WGA conjugates, related to Fig. 2 . (A) Fluorescence-pH plot of WGA-pHRho in solution; excitation wavelength, 550 nm. a.u., arbitrary units. (B) ΔF/F 0 -I plot of WGA-pHRho (red), homemade WGA-fluorescein (dark green), and WGA-fluorescein from ... More
Images
Changes in WGA-pHRho fluorescence requires exogenous expression of proton c...
Published: 24 January 2020
Figure S2. Changes in WGA-pHRho fluorescence requires exogenous expression of proton channels in CHO cells. Related to Fig. 2 . (A and B) Current–voltage relationships for (A) Hv-1 and (B) Shaker-IR (R362H/W434F) expressed in CHO cells. Hv-1 was held at −80 mV, and currents were elicited f... More
Images
Cell surface pH calibration of WGA-pHRho and WGA-fluorescein.  (A)  CHO cel...
Published: 24 January 2020
Figure 3. Cell surface pH calibration of WGA-pHRho and WGA-fluorescein. (A) CHO cells were labeled with either WGA-fluorescein or WGA-pHRho, and the change of fluorescence (ΔF/F 0 %) was plotted using pH standards (HEPES; 1 mM, open symbols; 10 mM, closed symbols); F o was chosen to be at the ... More
Images
Structured light microscopy of lactate-stimulated proton transport in rat v...
Published: 24 January 2020
Figure 4. Structured light microscopy of lactate-stimulated proton transport in rat ventricular myocytes labeled with WGA-pHRho. (A) A ∼750-nm illuminated z -plane of a WGA-pHRho–labeled ventricular myocyte ∼2 μm above the coverslip focal plane. (B) Time course of 10 mM lactate perfusion befo... More
Images
Proton efflux from primary neuron–astrocyte cocultures labeled with WGA-pHR...
Published: 24 January 2020
Figure 5. Proton efflux from primary neuron–astrocyte cocultures labeled with WGA-pHRho. (A) Merged Airyscan image of the neuronal and astrocyte planes labeled with WGA-pHRho (red) and DAPI (blue). (B) Pseudocolor image showing the averaged ΔF/F 0 of the regions of interest (ROI) in A after ... More
Images
Representative structures of each of the seven TRP subfamilies.  TRP channe...
Published: 23 January 2020
Figure 1. Representative structures of each of the seven TRP subfamilies. TRP channels are shown in ribbon diagrams viewed parallel to the membrane, with each subunit color-coded differently. Note, TRP channels are decorated with unique soluble domains outside the relatively conserved transmembr... More
Images
Determination of TRPV1 structures by single-particle cryo-EM.  (A)  The 3.4...
Published: 23 January 2020
Figure 2. Determination of TRPV1 structures by single-particle cryo-EM. (A) The 3.4 Å map of TRPV1 determined by single-particle cryo-EM is shown in a side view (left) and a cytoplasmic view (right). (B) The “resolution revolution” in cryo-EM has led to a recent explosion of structures deposi... More
Images
Chemistry and size of the selectivity filter of representative TRP channels...
Published: 23 January 2020
Figure 3. Chemistry and size of the selectivity filter of representative TRP channels and VGICs. TRP channel selectivity filters are diverse in both size and constituent residues. The diameters measured from two narrowest diagonally opposed oxygen atoms are shown at the top. The selectivity filt... More
Images
Structural elements that confer conformational flexibility to the pore lini...
Published: 23 January 2020
Figure 4. Structural elements that confer conformational flexibility to the pore lining S6 helix in K + and TRPV1 channels. (A) Gating hinges in KcsA (Gly99; red) and K v 2.1 (Pro-Val-Pro; shown in sticks) allow for splaying open of their activation gates. (B) A π-helix bulge, highlighted in... More
Images
Association of the S1–S4 domain with the pore domain in representative TRP ...
Published: 23 January 2020
Figure 5. Association of the S1–S4 domain with the pore domain in representative TRP and K v channels. TRP channel voltage-sensor like domain (VSLD; cyan) typically associates with pore domain from an adjacent subunit (orange; red) via a larger interface and thus remains relatively static durin... More
Images
Receptor sites for animal toxins targeting TRPV1 and VGICs.  (A)  The spide...
Published: 23 January 2020
Figure 6. Receptor sites for animal toxins targeting TRPV1 and VGICs. (A) The spider DkTx toxin latches onto the outer pore region of TRPV1 and stabilizes an open pore conformation. (B) Dc1a wedges into a cleft between the VSD and pore domains of Na v and impedes VSD movement. (C) Charybod... More
Images
Structures of thermo-sensitive TRP channels.  (A)  Two diagonally apposed T...
Published: 23 January 2020
Figure 7. Structures of thermo-sensitive TRP channels. (A) Two diagonally apposed TRPV1 subunits are shown in ribbon diagram, with regions proposed to undergo heat-evoked conformational changes highlighted with red. The phosphoinositide lipid predicted to be ejected from the vanilloid pocket up... More
Journal Articles
The Journal of General Physiology (2020) 152 (3): e201811998.
Published: 23 January 2020
Images
LC15 transgenic mice exhibit robust CUG exp  RNA expression in the heart  ....
Published: 22 January 2020
Figure 1. LC15 transgenic mice exhibit robust CUG exp RNA expression in the heart . (A) Schematic of transgene used to generate LC15 mice. An uninterrupted expanded CTG-repeat tract generated using a cell-free assembly procedure described previously ( Osborne and Thornton, 2008 ) was embedded... More
Images
Mbnl1 and Mbnl2 expression levels in hearts from LC15 mice.  (A)  Represent...
Published: 22 January 2020
Figure S1. Mbnl1 and Mbnl2 expression levels in hearts from LC15 mice. (A) Representative Western blot of Mbnl1 (top) and gapdh (bottom) in lysates from ventricular myocytes from WT and LC15 mice. (B) Relative Mbnl1 band intensity (normalized to gapdh) for lysates from ventricular myocytes is... More
Images
ECG analyses in anesthetized WT and LC15 mice.  (A)  Representative signal-...
Published: 22 January 2020
Figure 2. ECG analyses in anesthetized WT and LC15 mice. (A) Representative signal-averaged ECG traces aligned to the start of the Q-wave from 3-mo-old WT ( n = 11 mice; black) and LC15 ( n = 14 mice; red) littermate mice. The traces reflect the average of 3 s of continuous ECG recording from... More
Images
ECG analyses of conscious WT and LC15 mice using implanted radiotelemeters....
Published: 22 January 2020
Figure 3. ECG analyses of conscious WT and LC15 mice using implanted radiotelemeters. ECGs were recorded continuously for 60 h in mice surgically implanted with radiotelemetry devices (ETA-F10). (A) Plot of average heart rate (HR) over 5-min intervals during the 60 h of recording for a cohort ... More