1-20 of 27168
Follow your search
Access your saved searches in your account

Would you like to receive an alert when new items match your search?
Close Modal
Sort by
Journal Articles
The Journal of General Physiology (2020) 152 (2): e201912450.
Published: 22 January 2020
Images
LC15 transgenic mice exhibit robust CUG exp  RNA expression in the heart  ....
Published: 22 January 2020
Figure 1. LC15 transgenic mice exhibit robust CUG exp RNA expression in the heart . (A) Schematic of transgene used to generate LC15 mice. An uninterrupted expanded CTG-repeat tract generated using a cell-free assembly procedure described previously ( Osborne and Thornton, 2008 ) was embedded... More
Images
Mbnl1 and Mbnl2 expression levels in hearts from LC15 mice.  (A)  Represent...
Published: 22 January 2020
Figure S1. Mbnl1 and Mbnl2 expression levels in hearts from LC15 mice. (A) Representative Western blot of Mbnl1 (top) and gapdh (bottom) in lysates from ventricular myocytes from WT and LC15 mice. (B) Relative Mbnl1 band intensity (normalized to gapdh) for lysates from ventricular myocytes is... More
Images
ECG analyses in anesthetized WT and LC15 mice.  (A)  Representative signal-...
Published: 22 January 2020
Figure 2. ECG analyses in anesthetized WT and LC15 mice. (A) Representative signal-averaged ECG traces aligned to the start of the Q-wave from 3-mo-old WT ( n = 11 mice; black) and LC15 ( n = 14 mice; red) littermate mice. The traces reflect the average of 3 s of continuous ECG recording from... More
Images
ECG analyses of conscious WT and LC15 mice using implanted radiotelemeters....
Published: 22 January 2020
Figure 3. ECG analyses of conscious WT and LC15 mice using implanted radiotelemeters. ECGs were recorded continuously for 60 h in mice surgically implanted with radiotelemetry devices (ETA-F10). (A) Plot of average heart rate (HR) over 5-min intervals during the 60 h of recording for a cohort ... More
Images
LC15 mice exhibited increased sensitivity for arrhythmias following acute f...
Published: 22 January 2020
Figure 4. LC15 mice exhibited increased sensitivity for arrhythmias following acute flecainide administration. (A) Representative 1-s ECG recordings obtained from WT and LC15 mice immediately before (left), 2 min after (middle), and 5 min after (right) flecainide administration (20 mg/kg intrap... More
Images
AP recordings from isolated cardiac ventricular myocytes.  (A)  Representat...
Published: 22 January 2020
Figure 5. AP recordings from isolated cardiac ventricular myocytes. (A) Representative group-averaged AP traces from current clamp recordings of single ventricular myocytes from WT ( n = 5; black) and LC15 ( n = 8; red) mice. AP traces reflect the aligned signal average of 20 consecutive APs ... More
Images
Peak inward sodium current density is unaltered and the voltage dependence ...
Published: 22 January 2020
Figure 6. Peak inward sodium current density is unaltered and the voltage dependence of activation is right-shifted in ventricular myocytes from LC15 mice. (A) Representative superimposed inward sodium current traces measured during 300-ms voltage steps from −150 mV to +60 mV in 5-mV increments... More
Images
Voltage dependence of steady-state sodium channel inactivation is right-shi...
Published: 22 January 2020
Figure 7. Voltage dependence of steady-state sodium channel inactivation is right-shifted in ventricular myocytes from LC15 mice. (A) Representative inward sodium currents measured during 30-ms voltage steps to −40 mV (indicated by arrow) following 300-ms inactivating prepulses of varied amplit... More
Images
Peak outward potassium current density is reduced in ventricular myocytes f...
Published: 22 January 2020
Figure 8. Peak outward potassium current density is reduced in ventricular myocytes from LC15 mice. (A) Representative outward potassium currents measured during 4.5-s voltage steps from a holding potential of −70 mV in myocytes from WT (left; black) and LC15 (right; red) mice. Voltage steps fr... More
Images
Low 4-AP–sensitive potassium current density is reduced in myocytes from LC...
Published: 22 January 2020
Figure 9. Low 4-AP–sensitive potassium current density is reduced in myocytes from LC15 mice. (A) Representative outward potassium current traces in ventricular myocytes from WT (left; black) and LC15 (right; red) mice. Following recording in control solution, the bath solution was exchanged fo... More
Images
Splicing of fetal  Scn5a  exon 6A is not increased in heart tissue from LC1...
Published: 22 January 2020
Figure S2. Splicing of fetal Scn5a exon 6A is not increased in heart tissue from LC15 mice. Mutually exclusive Scn5a exon 6A/B expression was measured by PCR using complementary DNA made from total mRNA isolated from adult mouse heart tissue. PCR product containing the exon 6B splice variant... More
Images
Kv1.4 protein expression is reduced in ventricular myocytes from LC15 mice....
Published: 22 January 2020
Figure S3. Kv1.4 protein expression is reduced in ventricular myocytes from LC15 mice. (A) Representative Western blot of Kv1.4 (APC-007 antibody, Alomone) in lysates from ventricular myocytes isolated from WT and LC15 mice (top). A separate Western blot was performed using anti-gapdh on the lo... More
Images
ASIC membrane-spanning region.  (A)  Left, MD simulation system for rASIC2a...
Published: 17 January 2020
Figure 1. ASIC membrane-spanning region. (A) Left, MD simulation system for rASIC2a model based on PDB accession no. 4NTW (each subunit a different color) embedded in a lipid bilayer (cyan) in NaCl solution (water, red and white sticks; Na + and Cl − , orange and cyan balls). Right, magnifie... More
Images
Site-directed mutagenesis and relative ion permeability of homomeric ASIC1a...
Published: 17 January 2020
Figure 2. Site-directed mutagenesis and relative ion permeability of homomeric ASIC1a and ASIC2a. (A) Protocol and example recordings of current–voltage (I-V) relationship for WT rASIC2a and indicated mutants, assayed by proton-gated current during −80 → 60 mV ramp; current during voltage ramp i... More
Images
D  ifferences in the structures of cASIC1 and rASIC2a.  (A)  Pore size for ...
Published: 17 January 2020
Figure S1. D ifferences in the structures of cASIC1 and rASIC2a. (A) Pore size for cASIC1 (red) and equilibrated rASIC2a model (blue) calculated using HOLE ( Smart et al., 1996 ), based on the last 100 ns of each simulation, with error bars based on SEMs from five blocks. Positions of L7′, GAS... More
Images
Structural alignments of M2 helices. (A–F)  Structural alignments of M2 hel...
Published: 17 January 2020
Figure S2. Structural alignments of M2 helices. (A–F) Structural alignments of M2 helices between cASIC1 crystal structure (purple) and mASIC1a/mASIC1a/rASIC2a heteromers (blue) for Na + (left) and K + (right; A and B), mASIC1a/rASIC2a/rASIC2a heteromers (blue; C and D), and rASIC2a homomers (... More
Images
RMSD for the backbone of TM1 and TM2 (residues 45 to 75 and 425 to 456 in r...
Published: 17 January 2020
Figure S3. RMSD for the backbone of TM1 and TM2 (residues 45 to 75 and 425 to 456 in red, and residues 60 to 75 and 425 to 454 in blue; PDB accession no. 4NTW numbering). (A and B) mASIC1a/mASIC1a/rASIC2a heteromers with NaCl (A) and KCl (B). (C and D) mASIC1a/rASIC2a/rASIC2a heteromers ... More
Images
Behavior of the lower pore of cASIC1 and rASIC2a.  (A and B)  Distribution ...
Published: 17 January 2020
Figure S4. Behavior of the lower pore of cASIC1 and rASIC2a. (A and B) Distribution of single and double occupancy for sites formed by E18′ and D21′ for cASIC1 (A) and rASIC2a (B) channels (mean ± SEM). Single ion occupancy is preferred in cASIC1 and rASIC2a for both Na + and K + ; however, do... More
Images
PMFs for Na +  and K + .  (A–  F  )  PMFs for Na +  (orange) and K +  (blue...
Published: 17 January 2020
Figure S5. PMFs for Na + and K + . (A– F ) PMFs for Na + (orange) and K + (blue) permeating the pore of mASIC1a/mASIC1a/rASIC2a heteromers (A and D) from US simulations (A) and free simulations (D); mASIC1a/rASIC2a/rASIC2a heteromers (B and E) from US simulations (B) and free simulations (... More