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Journal Articles
The Journal of General Physiology (2020) 152 (3): e201811998.
Published: 23 January 2020
Images
Representative structures of each of the seven TRP subfamilies.  TRP channe...
Published: 23 January 2020
Figure 1. Representative structures of each of the seven TRP subfamilies. TRP channels are shown in ribbon diagrams viewed parallel to the membrane, with each subunit color-coded differently. Note, TRP channels are decorated with unique soluble domains outside the relatively conserved transmembr... More
Images
Determination of TRPV1 structures by single-particle cryo-EM.  (A)  The 3.4...
Published: 23 January 2020
Figure 2. Determination of TRPV1 structures by single-particle cryo-EM. (A) The 3.4 Å map of TRPV1 determined by single-particle cryo-EM is shown in a side view (left) and a cytoplasmic view (right). (B) The “resolution revolution” in cryo-EM has led to a recent explosion of structures deposi... More
Images
Chemistry and size of the selectivity filter of representative TRP channels...
Published: 23 January 2020
Figure 3. Chemistry and size of the selectivity filter of representative TRP channels and VGICs. TRP channel selectivity filters are diverse in both size and constituent residues. The diameters measured from two narrowest diagonally opposed oxygen atoms are shown at the top. The selectivity filt... More
Images
Structural elements that confer conformational flexibility to the pore lini...
Published: 23 January 2020
Figure 4. Structural elements that confer conformational flexibility to the pore lining S6 helix in K + and TRPV1 channels. (A) Gating hinges in KcsA (Gly99; red) and K v 2.1 (Pro-Val-Pro; shown in sticks) allow for splaying open of their activation gates. (B) A π-helix bulge, highlighted in... More
Images
Association of the S1–S4 domain with the pore domain in representative TRP ...
Published: 23 January 2020
Figure 5. Association of the S1–S4 domain with the pore domain in representative TRP and K v channels. TRP channel voltage-sensor like domain (VSLD; cyan) typically associates with pore domain from an adjacent subunit (orange; red) via a larger interface and thus remains relatively static durin... More
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Receptor sites for animal toxins targeting TRPV1 and VGICs.  (A)  The spide...
Published: 23 January 2020
Figure 6. Receptor sites for animal toxins targeting TRPV1 and VGICs. (A) The spider DkTx toxin latches onto the outer pore region of TRPV1 and stabilizes an open pore conformation. (B) Dc1a wedges into a cleft between the VSD and pore domains of Na v and impedes VSD movement. (C) Charybod... More
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Structures of thermo-sensitive TRP channels.  (A)  Two diagonally apposed T...
Published: 23 January 2020
Figure 7. Structures of thermo-sensitive TRP channels. (A) Two diagonally apposed TRPV1 subunits are shown in ribbon diagram, with regions proposed to undergo heat-evoked conformational changes highlighted with red. The phosphoinositide lipid predicted to be ejected from the vanilloid pocket up... More
Images
LC15 transgenic mice exhibit robust CUG exp  RNA expression in the heart  ....
Published: 22 January 2020
Figure 1. LC15 transgenic mice exhibit robust CUG exp RNA expression in the heart . (A) Schematic of transgene used to generate LC15 mice. An uninterrupted expanded CTG-repeat tract generated using a cell-free assembly procedure described previously ( Osborne and Thornton, 2008 ) was embedded... More
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Mbnl1 and Mbnl2 expression levels in hearts from LC15 mice.  (A)  Represent...
Published: 22 January 2020
Figure S1. Mbnl1 and Mbnl2 expression levels in hearts from LC15 mice. (A) Representative Western blot of Mbnl1 (top) and gapdh (bottom) in lysates from ventricular myocytes from WT and LC15 mice. (B) Relative Mbnl1 band intensity (normalized to gapdh) for lysates from ventricular myocytes is... More
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ECG analyses in anesthetized WT and LC15 mice.  (A)  Representative signal-...
Published: 22 January 2020
Figure 2. ECG analyses in anesthetized WT and LC15 mice. (A) Representative signal-averaged ECG traces aligned to the start of the Q-wave from 3-mo-old WT ( n = 11 mice; black) and LC15 ( n = 14 mice; red) littermate mice. The traces reflect the average of 3 s of continuous ECG recording from... More
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ECG analyses of conscious WT and LC15 mice using implanted radiotelemeters....
Published: 22 January 2020
Figure 3. ECG analyses of conscious WT and LC15 mice using implanted radiotelemeters. ECGs were recorded continuously for 60 h in mice surgically implanted with radiotelemetry devices (ETA-F10). (A) Plot of average heart rate (HR) over 5-min intervals during the 60 h of recording for a cohort ... More
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LC15 mice exhibited increased sensitivity for arrhythmias following acute f...
Published: 22 January 2020
Figure 4. LC15 mice exhibited increased sensitivity for arrhythmias following acute flecainide administration. (A) Representative 1-s ECG recordings obtained from WT and LC15 mice immediately before (left), 2 min after (middle), and 5 min after (right) flecainide administration (20 mg/kg intrap... More
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AP recordings from isolated cardiac ventricular myocytes.  (A)  Representat...
Published: 22 January 2020
Figure 5. AP recordings from isolated cardiac ventricular myocytes. (A) Representative group-averaged AP traces from current clamp recordings of single ventricular myocytes from WT ( n = 5; black) and LC15 ( n = 8; red) mice. AP traces reflect the aligned signal average of 20 consecutive APs ... More
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Peak inward sodium current density is unaltered and the voltage dependence ...
Published: 22 January 2020
Figure 6. Peak inward sodium current density is unaltered and the voltage dependence of activation is right-shifted in ventricular myocytes from LC15 mice. (A) Representative superimposed inward sodium current traces measured during 300-ms voltage steps from −150 mV to +60 mV in 5-mV increments... More
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Voltage dependence of steady-state sodium channel inactivation is right-shi...
Published: 22 January 2020
Figure 7. Voltage dependence of steady-state sodium channel inactivation is right-shifted in ventricular myocytes from LC15 mice. (A) Representative inward sodium currents measured during 30-ms voltage steps to −40 mV (indicated by arrow) following 300-ms inactivating prepulses of varied amplit... More
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Peak outward potassium current density is reduced in ventricular myocytes f...
Published: 22 January 2020
Figure 8. Peak outward potassium current density is reduced in ventricular myocytes from LC15 mice. (A) Representative outward potassium currents measured during 4.5-s voltage steps from a holding potential of −70 mV in myocytes from WT (left; black) and LC15 (right; red) mice. Voltage steps fr... More
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Low 4-AP–sensitive potassium current density is reduced in myocytes from LC...
Published: 22 January 2020
Figure 9. Low 4-AP–sensitive potassium current density is reduced in myocytes from LC15 mice. (A) Representative outward potassium current traces in ventricular myocytes from WT (left; black) and LC15 (right; red) mice. Following recording in control solution, the bath solution was exchanged fo... More
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Splicing of fetal  Scn5a  exon 6A is not increased in heart tissue from LC1...
Published: 22 January 2020
Figure S2. Splicing of fetal Scn5a exon 6A is not increased in heart tissue from LC15 mice. Mutually exclusive Scn5a exon 6A/B expression was measured by PCR using complementary DNA made from total mRNA isolated from adult mouse heart tissue. PCR product containing the exon 6B splice variant... More
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Kv1.4 protein expression is reduced in ventricular myocytes from LC15 mice....
Published: 22 January 2020
Figure S3. Kv1.4 protein expression is reduced in ventricular myocytes from LC15 mice. (A) Representative Western blot of Kv1.4 (APC-007 antibody, Alomone) in lysates from ventricular myocytes isolated from WT and LC15 mice (top). A separate Western blot was performed using anti-gapdh on the lo... More