Chahine highlights new work that exploits the increased expression of human CaV1.1 at the plasma membrane after coexpression with Stac3.
Milestone in Physiology
A retrospective on the scientific importance and impact of Hecht, Shlaer, and Pirenne’s classic 1942 paper, “Energy, Quanta, and Vision.”
Hille recounts the founding years of JGP, including key articles that advanced the field of membrane permeation and ion selectivity.
The mollusk Pecten irradians possesses ciliary photoreceptors that operate with an atypical mechanism. Arenas et al. reveal that a recently uncovered opsin type is the functional visual pigment in these photoreceptors and couples to Go, in contrast to other types of photoreceptor.
P-glycoprotein contributes to multidrug resistance by exporting a broad range of substrates across the cell membrane. Using molecular dynamics simulations, Barreto-Ojeda et al. identify key lipid-binding sites and reveal lipid access pathways toward the cavity of the transporter.
This study on serotonin transporters shows that amphetamine-induced monoamine release requires cooperative substrate and cosubstrate binding. A kinetic model is presented that can account qualitatively and quantitatively for the releasing action of amphetamines.
Nicotine acetylcholine receptors can form countless heteromeric stoichiometries from a common set of subunits. Ahring et al. present the limitations of subunit concatenation and establish a refinement that achieves substantiated expression of uniform receptor pools from complex stoichiometric origins.
Stac3 enhances expression of human CaV1.1 in Xenopus oocytes and reveals gating pore currents in HypoPP mutant channels
Hypokalemic periodic paralysis (HypoPP) is thought to be caused by an aberrant inward current through the voltage sensors of mutant Na+ or Ca2+ channels. Wu et al. use Stac3 to enhance the membrane expression of two HypoPP CaV1.1 mutants in oocytes and find that both support gating pore currents.
Cav2.3 Ca2+ channels are subject to cytosolic regulation, which has been difficult to characterize in native cells. Neumaier et al. demonstrate the role of phosphorylation in the function of these channels and suggest a close relationship between voltage dependence and the phosphorylation state.