The total CO2 produced by aseptic Drosophila cultures during the entire duration of life has been determined at 15°, 26°, and 30°C. in the dark and at 22–26°C. in the light.

The total amount of CO2 produced is not constant but is greater at 15° than at 26° or 30°, and is much greater in the light than in the dark.

The total duration of life, therefore, is not determined by the time required to produce a limiting amount of CO2.

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