The nonionic detergent Brij 58 eliminates irreversibly the capability of the sarcoplasmic reticulum (SR) of skinned crayfish muscle fibers to sequester Ca and to release it under appropriate stimulation. In contrast to deoxycholate (DOC) which causes an irreversible diminution of tension as well, Brij 58 does not affect the contractile proteins. Comparison of the time-course of tension development before and after Brij treatment demonstrates that Ca is accessible to the contractile proteins more rapidly after the SR is destroyed but, nevertheless, much more slowly than is predicted for free diffusion of Ca in the myoplasm. Slowing apparently results because of the presence of ca 1 mmol/kg fiber of myoplasmic Ca-binding sites that remain after Ca uptake of the SR is eliminated. A theoretical model is presented which allows for the effects of binding sites and of an unstirred layer in the vicinity of the fiber on Ca diffusion into the myoplasm.
Article|
February 01 1974
Calcium Binding and Tension Development in Detergent-Treated Muscle Fibers
M. Orentlicher,
M. Orentlicher
From the Laboratory of Neurophysiology, Department of Neurology and Department of Anatomy, College of Physicians and Surgeons, Columbia University, New York, New York 10032
Search for other works by this author on:
J. P. Reuben,
J. P. Reuben
From the Laboratory of Neurophysiology, Department of Neurology and Department of Anatomy, College of Physicians and Surgeons, Columbia University, New York, New York 10032
Search for other works by this author on:
H. Grundfest,
H. Grundfest
From the Laboratory of Neurophysiology, Department of Neurology and Department of Anatomy, College of Physicians and Surgeons, Columbia University, New York, New York 10032
Search for other works by this author on:
P. W. Brandt
P. W. Brandt
From the Laboratory of Neurophysiology, Department of Neurology and Department of Anatomy, College of Physicians and Surgeons, Columbia University, New York, New York 10032
Search for other works by this author on:
M. Orentlicher
From the Laboratory of Neurophysiology, Department of Neurology and Department of Anatomy, College of Physicians and Surgeons, Columbia University, New York, New York 10032
J. P. Reuben
From the Laboratory of Neurophysiology, Department of Neurology and Department of Anatomy, College of Physicians and Surgeons, Columbia University, New York, New York 10032
H. Grundfest
From the Laboratory of Neurophysiology, Department of Neurology and Department of Anatomy, College of Physicians and Surgeons, Columbia University, New York, New York 10032
P. W. Brandt
From the Laboratory of Neurophysiology, Department of Neurology and Department of Anatomy, College of Physicians and Surgeons, Columbia University, New York, New York 10032
Received:
May 07 1973
Online Issn: 1540-7748
Print Issn: 0022-1295
1974
J Gen Physiol (1974) 63 (2): 168–186.
Article history
Received:
May 07 1973
Citation
M. Orentlicher, J. P. Reuben, H. Grundfest, P. W. Brandt; Calcium Binding and Tension Development in Detergent-Treated Muscle Fibers . J Gen Physiol 1 February 1974; 63 (2): 168–186. doi: https://doi.org/10.1085/jgp.63.2.168
Download citation file: