Parietovisceral ganglia from Aplysia californica were incubated in medium containing leucine-3H. Single, identified nerve cell somas were isolated from the ganglia, and their proteins extracted and separated by electrophoresis on 5% SDS-polyacrylamide gels. The distribution of total or newly synthesized proteins from the single neurons was determined by staining or slicing and liquid scintillation counting of the gels. Experiments showed that: (a) a number of proteins were being synthesized in abundance in the nerve cells; (b) different, identified neurons showed reproducibly different labeling patterns in the gels; (c) cells R2 and R15, which showed different distributions of radioactivity in the gels, had similar staining patterns; and (d) there was significant incorporation into material of high (>75,000) molecular weight in most of the cells.
Article|
January 01 1971
Molecular Weight Distribution of Proteins Synthesized in Single, Identified Neurons of Aplysia
David L. Wilson
David L. Wilson
From the Division of Biology, California Institute of Technology, Pasadena, California 91109
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David L. Wilson
From the Division of Biology, California Institute of Technology, Pasadena, California 91109
Received:
May 02 1970
Online Issn: 1540-7748
Print Issn: 0022-1295
Copyright © 1971 by The Rockefeller University Press
1971
J Gen Physiol (1971) 57 (1): 26–40.
Article history
Received:
May 02 1970
Citation
David L. Wilson; Molecular Weight Distribution of Proteins Synthesized in Single, Identified Neurons of Aplysia . J Gen Physiol 1 January 1971; 57 (1): 26–40. doi: https://doi.org/10.1085/jgp.57.1.26
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