The effects of metabolic inhibitors and ouabain on alanine transport across rabbit ileum, in vitro, have been investigated. Net transport of alanine and Na across short-circuited segments of ileum is virtually abolished by cyanide, 2,4-dinitrophenol, iodoacetate, and ouabain. However, these inhibitors do not markedly depress alanine influx from the mucosal solution, across the brush border, into the intestinal epithelium, and they do not significantly affect the Na dependence of this entry process. The results of this investigation indicate that: (a) the Na dependence of alanine influx does not reflect a mechanism in which the sole function of Na is to link metabolic energy directly to the influx process; and (b) the inhibition of net alanine transport across intestine is, in part, the result of an increased rate coefficient for alanine efflux out of the cell across the brush border. Although these findings do not exclude a direct link between metabolic energy and alanine efflux, the increased efflux may be the result of the increased intracellular Na concentration in the presence of these inhibitors. The results of these studies are qualitatively consistent with a model for alanine transport across the brush border which does not include a direct link to metabolic energy.
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November 01 1967
Effect of Inhibitors on Alanine Transport in Isolated Rabbit Ileum
Ronald A. Chez,
Ronald A. Chez
From the Biophysical Laboratory, Harvard Medical School, Boston, Massachusetts 02115.
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Richard R. Palmer,
Richard R. Palmer
From the Biophysical Laboratory, Harvard Medical School, Boston, Massachusetts 02115.
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Stanley G. Schultz,
Stanley G. Schultz
From the Biophysical Laboratory, Harvard Medical School, Boston, Massachusetts 02115.
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Peter F. Curran
Peter F. Curran
From the Biophysical Laboratory, Harvard Medical School, Boston, Massachusetts 02115.
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Ronald A. Chez
From the Biophysical Laboratory, Harvard Medical School, Boston, Massachusetts 02115.
Richard R. Palmer
From the Biophysical Laboratory, Harvard Medical School, Boston, Massachusetts 02115.
Stanley G. Schultz
From the Biophysical Laboratory, Harvard Medical School, Boston, Massachusetts 02115.
Peter F. Curran
From the Biophysical Laboratory, Harvard Medical School, Boston, Massachusetts 02115.
Dr. Chez's present address is the Department of Obstetrics and Gynecology, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania 15213. Dr. Schultz's present address is the Department of Physiology, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania. Dr. Curran's present address is the Department of Physiology, Yale University School of Medicine, New Haven, Connecticut 06510
Received:
April 17 1967
Online Issn: 1540-7748
Print Issn: 0022-1295
Copyright © 1967 by The Rockefeller University Press
1967
J Gen Physiol (1967) 50 (10): 2357–2375.
Article history
Received:
April 17 1967
Citation
Ronald A. Chez, Richard R. Palmer, Stanley G. Schultz, Peter F. Curran; Effect of Inhibitors on Alanine Transport in Isolated Rabbit Ileum . J Gen Physiol 1 November 1967; 50 (10): 2357–2375. doi: https://doi.org/10.1085/jgp.50.10.2357
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