Mice lacking functional large-conductance voltage- and Ca2+-activated K+ channels (BK channels) are viable but have motor deficits including ataxia and weakness. The cause of weakness is unknown. In this study, we discovered, in vivo, that skeletal muscle in mice lacking BK channels (BK−/−) was weak in response to nerve stimulation but not to direct muscle stimulation, suggesting a failure of neuromuscular transmission. Voltage-clamp studies of the BK−/− neuromuscular junction (NMJ) revealed a reduction in evoked endplate current amplitude and the frequency of spontaneous vesicle release compared with WT littermates. Responses to 50-Hz stimulation indicated a reduced probability of vesicle release in BK−/− mice, suggestive of lower presynaptic Ca2+ entry. Pharmacological block of BK channels in WT NMJs did not affect NMJ function, surprisingly suggesting that the reduced vesicle release in BK−/− NMJs was not due to loss of BK channel–mediated K+ current. Possible explanations for our data include an effect of BK channels on development of the NMJ, a role for BK channels in regulating presynaptic Ca2+ current or the effectiveness of Ca2+ in triggering release. Consistent with reduced Ca2+ entry or effectiveness of Ca2+ in triggering release, use of 3,4-diaminopyridine to widen action potentials normalized evoked release in BK−/− mice to WT levels. Intraperitoneal application of 3,4-diaminopyridine fully restored in vivo nerve-stimulated muscle force in BK−/− mice. Our work demonstrates that mice lacking BK channels have weakness due to a defect in vesicle release at the NMJ.
Large-conductance voltage- and Ca2+-activated K+ channels (BK, Maxi-K, slo1, or KCa1.1 channels) are widely expressed in the nervous system and have been shown to play a central role in the regulation of excitability (Salkoff et al., 2006; Berkefeld et al., 2010; Wang et al., 2014). Mice lacking functional BK channels have impairments that include increased mortality, hearing loss, hypertension, bladder dysfunction, and motor deficits (Meredith et al., 2004; Rüttiger et al., 2004; Sausbier et al., 2005; Halm et al., 2017). The motor deficits include tremor, problems with gait, weak grip, and decreased ability to stay on the rotarod. In humans, mutations in the gene that encodes the BK channel (KCNMA1) are linked with epilepsy, movement disorders, and hypotonia (Bailey et al., 2019). Broadly, motor phenotypes caused by BK channel mutations or loss have been largely attributed to cerebellar dysfunction (Meredith et al., 2004; Sausbier et al., 2004; Chen et al., 2010; Typlt et al., 2013).
Despite evidence suggesting that mice lacking BK channels are weak, we are unaware of studies to determine the mechanism underlying weakness. BK channels are expressed in skeletal muscle (Tricarico et al., 2004; Dinardo et al., 2012) and are present at the neuromuscular junction (NMJ), where they have been suggested to regulate synaptic function (Robitaille and Charlton, 1992; Robitaille et al., 1993; Vatanpour and Harvey, 1995; Sugiura and Ko, 1997; Pattillo et al., 2001; Flink and Atchison, 2003; Ford and Davis, 2014). The presence of BK channels in muscle and at the NMJ suggests that loss of BK channels in the neuromuscular system could contribute to weakness.
We measured muscle force triggered by stimulation of the sciatic nerve in vivo and found a significant reduction of specific force in BK−/− mice relative to WT. Notably, force generation was normal when the muscle was directly stimulated, suggesting a defect in neuromuscular transmission. Ex vivo voltage-clamp measurements of neuromuscular function revealed a dramatic reduction in the release of synaptic vesicles. Acute pharmacological block of BK channels did not affect NMJ function in WT mice, strongly suggesting that the reduction of vesicle release in BK−/− mice is independent of BK channel ion current. Block of voltage-gated K+ channels with 3,4-diaminopyridine (3,4-DAP) to prolong neuronal action potentials normalized NMJ function ex vivo and increased force production in vivo. Together, these data suggest that, in the absence of BK channels, Ca2+ entry is either reduced or less effective in triggering vesicle release.
Materials and methods
All procedures involving animals were approved by the Wright State Institutional Animal Care and Use Committee.
BK−/− mice were obtained from Andrea Meredith at the University of Maryland (College Park, MD) and a breeding colony established at Wright State University (Dayton, OH). WT littermates were used as controls.
Force measurements were performed as previously reported (Dupont et al., 2019). Mice were anesthetized with isoflurane using a low-flow anesthesia system (SS-01; Kent Scientific), with body temperature maintained at ∼35°C using a heat lamp and temperature probe. The plantar flexor muscles (including the lateral and medial gastrocnemius, plantaris, and soleus muscles) and sciatic nerve were exposed by removing the surrounding skin. To minimize the influence of adjacent muscles, the common peroneal and tibial nerves were crushed. During surgery, the muscles were bathed in physiological saline to prevent them from drying out. Mice were then transferred to a custom 3D-printed platform designed to eliminate movement during in vivo force recordings. The mouse limb was stabilized with vertical supports and by pinning the limb into recessed areas in the platform that were filled with Sylgard. The platform was mounted to a micromanipulator (XR25/M; Thorlabs). The proximal end of the plantar flexor muscles (Achilles tendon) was attached to the lever of the force transducer (300D-305C dual-mode muscle lever; Aurora Scientific) using 5.0 or 6.0 silk suture and a modified Miller’s knot. Optimal length was obtained by measuring the maximum twitch force while lengthening the muscle using the micromanipulator.
Nerve-evoked contractions were stimulated with platinum electrodes resting on the sciatic nerve. Direct muscle contractions were stimulated via platinum electrodes positioned above and below the plantar flexor muscles. For muscle-stimulated contractions, neuromuscular transmission was blocked using ∼150 µl of 0.5 mg/ml α-bungarotoxin (α-BTX) injected into the gastrocnemius muscles. After injection of α-BTX, neuromuscular block was confirmed by ensuring that the twitch force in response to nerve stimulation was ≤25% of maximum muscle-stimulated twitch. Stimulus amplitude and the pulse width was ≤5 V at 1 ms or ≤50 V at 1.5 ms for nerve-stimulated and muscle-stimulated contractions, respectively. A Dagan S-900 Stimulator and S-910 Stimulus Isolation Unit were used for stimulation. Muscle force was recorded and digitized using pClamp10 software (Molecular Devices). For experiments using intraperitoneal injections of 3,4-DAP (Sigma-Aldrich), the drug was diluted with water and injected at a dose of 8 mg/kg animal weight. The average volume injected was 100 µl. 3,4-DAP was injected immediately after the mice were anesthetized. Force-frequency data were fit with a Boltzmann equation. Data were analyzed with OriginPro 2019 (OriginLab). Data are shown as mean ± SEM unless otherwise stated.
Voltage-clamp recordings of endplate currents (EPCs)
Mice were sacrificed using CO2 inhalation, and the tibialis anterior muscle was removed. For most experiments, the recording chamber was continuously perfused with Ringer solution containing (in mM/liter) 118 NaCl, 3.5 KCl, 2 CaCl2, 0.7 MgSO4, 26.2 NaHCO3, 1.7 NaH2PO4, and 5.5 glucose (pH 7.3–7.4, 20–22°C) equilibrated with 95% O2 and 5% CO2. For experiments in low Ca2+/high Mg2+, the CaCl2 was reduced to 0.5 mM and the MgSO4 was increased to 2 mM. Endplate recordings were performed as previously described (Wang et al., 2016; Wang and Rich, 2018). Briefly, the tibialis anterior muscle was pinned in a dish and stained with 10 µM 4-(4-diethylaminostyryl)-N-methylpyridinium iodide (Invitrogen) to visualize the NMJs. To eliminate contraction, 1–3 µM µ-conotoxin GIIIB (Peptide Institute) was applied to block muscle Na+ channels. Muscle fibers were voltage-clamped at −70 mV. For all experiments, quantal content was calculated by dividing peak EPC amplitude by peak miniature EPC (mEPC) current amplitude. 3,4-DAP was applied at a dose of 100 µM.
Muscle force recordings were done at physiological temperature, whereas voltage-clamp recordings of NMJ function were performed at room temperature. As this difference in temperature could lead to different results, we confirmed the efficacy of 3,4-DAP in correcting NMJ transmission in vivo.
The lumbosacral spinal cord was removed from euthanized WT and BK−/− mice, frozen in liquid nitrogen, and stored at −80°C. Total RNA was isolated from spinal cord samples using the TRIzol technique. 1 µg RNA was reverse transcribed to synthesize cDNA with random primers. To quantify various Kcna (Kv1) and Kcnc (Kv3) channels and β2-microglobulin mRNA levels, real-time quantitative reverse transcription (RT)-PCR was performed with an MJ Research DNA engine Opticon 2 using Taqman gene-specific primers and probes (Kcna1, Mm00439977.s1; Kcna2, Mm00434584.s1; Kcna4, Mm00435241.s1; and β2-microglobulin, Mm00437762.m1) from Applied Biosystems (Thermo Fisher Scientific). Cycle thresholds were normalized to β2-microglobulin cycle thresholds and analyzed using the ΔΔCT method (Livak and Schmittgen, 2001).
Experimental design and statistical analysis
For voltage-clamp experiments, at least seven NMJs were recorded per muscle. For experiments in which drugs were given, the same muscle was studied before and after drug treatment. Nested analysis of variance (SYSTAT; Systat Software) was used for comparing the effect of drugs and other experimental manipulations. Averaged results are expressed as mean ± SEM. P values < 0.05 and < 0.01 are denoted by one and two asterisks, respectively.
For force measurements, four WT and six BK−/− mice were used. For force experiments with 3,4-DAP, five WT and four BK−/− mice were used. Force–frequency curves were generated and then fit with a Boltzmann sigmoidal curve. One-way ANOVA was performed to compare nerve versus muscle stimulation as well as with and without the drug. All error bars shown are ± SEM.
Muscle weakness in BK−/− mice was assessed in the plantar flexor (medial and lateral gastrocnemius, plantaris, and soleus) muscles by measuring in vivo muscle force via both sciatic nerve stimulation and direct muscle stimulation. To achieve direct muscle stimulation, α-BTX was injected into the muscle to block neuromuscular transmission and stimulating electrodes were placed above and below the plantar flexor muscles. An important consideration in assessing force production is muscle mass. The average weight of the plantar flexor muscles from the 2–4-mo-old BK−/− mice (0.14 ± 0.01 g) used in this study trended lower than in the age-matched WT mice (0.18 ± 0.01 g; P = 0.088). The potential impact of this on overall motor function depends on body weight. A previous study showed that BK−/− mice were smaller than WT littermates (Halm et al., 2017). In this study, the weight of BK−/− mice (19.7 ± 0.9 g) was also significantly lower than that of WT mice (24.6 ± 1.7 g; P = 0.025). The plantar flexor muscle weight to body weight ratio of BK−/− mice (0.0073 ± 0.0003) was not significantly different from WT (0.0073 ± 0.0001; P = 0.975), indicating that the reduction in BK−/− muscle weight was proportional to the reduction in body weight. To account for changes in muscle mass, we normalized the force to muscle weight (specific force).
Muscle force triggered by single action potentials (twitch), as well as by 30- and 60-Hz stimulation, is shown for BK−/− and WT muscle in response to stimulation of the sciatic nerve and direct muscle stimulation (Fig. 1 A). The peak twitch force of BK−/− muscle in response to nerve stimulation (4.00 ± 0.40 N/g) was significantly lower than in BK−/− muscle stimulated directly (7.60 ± 0.66 N/g; P = 1.54 × 10−4), WT muscle stimulated via nerve (6.82 ± 0.11 N/g; P = 0.005), or WT muscle stimulated directly (6.26 ± 0.21 N/g; P = 0.023; Fig. 1 B; one-way ANOVA, Tukey correction). The force–frequency relationship for WT muscle showed that nerve and direct muscle stimulation generated the same force at all frequencies (0.3–80 Hz; Fig. 1 C). In contrast, nerve-stimulated BK−/− muscle was weak at all frequencies compared with direct muscle stimulation. These data indicate that BK−/− muscle is significantly weak upon nerve stimulation but is capable of generating normal force at all frequencies with direct muscle stimulation. This strongly suggests that a defect in the motor axon or NMJ function is responsible for weakness in BK−/− mice.
To probe function of the NMJ, we performed voltage-clamp recordings of EPCs from the tibialis anterior muscle, as previously described (Wang et al., 2016). During these recordings, there was no failure of action potential invasion into the presynaptic terminal. These findings argue against a defect in function of the motor axon. EPC amplitude was reduced by >60% in BK−/− mice (Fig. 2 A). The reduction in EPC amplitude was not due to a change in the mEPC, as the mEPC amplitude was slightly larger in BK−/− than WT mice (Fig. 2 B). The normal size of the mEPCs strongly suggests that postsynaptic acetylcholine receptors are expressed at normal levels in BK−/− mice. These data indicated that the number of vesicles released in response to a presynaptic action potential (quantal content) was reduced by close to 60%. This reduction was accompanied by a 70% reduction in the frequency of mEPCs in BK−/− muscle (1.1 ± 0.5 Hz) compared with WT (3.6 ± 0.3 Hz; Fig. 2).
Both the probability of vesicle release and the number of releasable vesicles contribute to quantal content (Bennett et al., 1975; Wang et al., 2010a, 2010b). We inferred the probability of release by examining short-term synaptic plasticity during repetitive stimulation. WT NMJs and other synapses with a high probability of release undergo depression (reduced release with repetitive stimulation), whereas NMJs and other synapses with a low probability of release undergo facilitation (increased release with repetitive stimulation; Zucker and Regehr, 2002; Wang et al., 2004). BK−/− NMJs underwent facilitation rather than depression during repetitive stimulation (Fig. 3). These data suggest that a reduction in the probability of release is an important contributor to the reduction in vesicle release in BK−/− NMJs.
The reduction in probability of vesicle release was a surprise, as loss of BK channels at the NMJ has generally been proposed to prolong the action potentials and increase Ca2+ entry (Robitaille and Charlton, 1992; Robitaille et al., 1993; Vatanpour and Harvey, 1995; Flink and Atchison, 2003; Ford and Davis, 2014; see, however, Pattillo et al., 2001). The reduction in the probability of release caused us to question whether the impact of lost BK channels was due to the loss of BK-mediated K+ current. To investigate this, we applied three different BK channel blockers that have been used to probe channel function at the NMJ: 400 nM iberiotoxin, 10 µM paxilline, and 400 nM charybdotoxin (Robitaille and Charlton, 1992; Robitaille et al., 1993; Vatanpour and Harvey, 1995; Sugiura and Ko, 1997; Pattillo et al., 2001; Flink and Atchison, 2003). None of the toxins had a detectable effect on either quantal content or frequency of mEPCs (Table 1). Iberiotoxin and charybdotoxin were both purchased and tested from two vendors. These data suggest that K+ current through BK channels has little to no effect on the function of the adult mouse NMJ.
Our results differ from previous studies of the mouse NMJ that suggested significantly increased release of vesicles following block of BK channels (Vatanpour and Harvey, 1995; Flink and Atchison, 2003). One previous study was performed using low extracellular Ca2+ to eliminate twitch (Vatanpour and Harvey, 1995). To determine whether this experimental difference could account for the difference in results, we tested the efficacy of BK channel blockers in a low Ca2+/high Mg2+ solution similar to that used by Vatanpour and Harvey. None of the BK channel blockers tested increased quantal content in low-Ca2+/high-Mg2+ solution (Table 2).
In Drosophila melanogaster, voltage-gated shaker (Kv) channels were shown to be up-regulated following knockout of BK channels, such that block of Kv channels with 4-aminopyridine caused a large increase in transmitter release at the NMJ (Lee et al., 2008; see, however, Warbington et al., 1996). This treatment is thought to increase Ca2+ entry and vesicle release because the block of Kv channels widens the presynaptic action potential (Wang et al., 2016; Meriney et al., 2018; Ng et al., 2017). We tested the effect of the Kv channel blocker 3,4-DAP in WT and BK−/− NMJs (Fig. 4). Following the application of 3,4-DAP, the EPC amplitude and duration increased at both WT and BK−/− NMJs (Fig. 4 A). In WT NMJs, the increase in EPC amplitude was due to an increase in mEPC amplitude such that quantal content was minimally changed. While others have seen a similar increase in amplitude of miniature endplate potentials following treatment with 3,4-DAP (Mori et al., 2012; Ng et al., 2017), the mechanism underlying the increase has not been determined. If 3,4-DAP inhibits acetylcholinesterase, the increase in mEPC amplitude would be associated with a slowing of mEPC decay. Following addition of 3,4-DAP, the time constant of mEPC decay was significantly prolonged for WT from 1.05 ± 0.04 ms (five mice, 42 NMJs) to 1.35 ± 0.04 ms (41 NMJs, P < 0.01) and for BK−/− from 0.93 ± 0.03 ms (42 NMJs) to 1.38 ± 0.03 ms (five mice, 49 NMJs, P < 0.01). These data support the possibility that 3,4-DAP has two effects on NMJ function that increase amplitude of the EPC: block of presynaptic Kv channels and partial block of acetylcholinesterase.
In BK−/− NMJs, a dramatic increase in EPC amplitude was due to both an increase in mEPC amplitude and a more than twofold increase of quantal content (Fig. 4 B). The block of Kv channels with 3,4-DAP also increased the frequency of spontaneous release, as has been reported by others (Ng et al., 2017). However, it did not eliminate the difference in frequency of spontaneous release of vesicles in WT versus BK−/− NMJs (Fig. 4). These data suggest that the defect in vesicle release at BK−/− NMJs cannot be fully explained by reduced voltage-dependent Ca2+ entry during action potentials. Nonetheless, the difference in quantal content between WT and BK−/− NMJs could be eliminated by 3,4-DAP, which strongly suggests the number of releasable vesicles is normal, but the probability of release is reduced.
Given the similarity of our findings to those in Drosophila, we examined whether up-regulation of Kv channels occurred after elimination of BK channels. As an initial screen, we assayed levels of several Kv1 and Kv3 isoforms expressed by motor neurons (Brooke et al., 2004; Duflocq et al., 2011). The mRNA levels in the lumbosacral spinal cord of WT versus BK−/− mice were assessed by real-time quantitative RT-PCR relative to β-2-microglobulin mRNA for normalization purposes. There was no up-regulation of the Kv1 and Kv3 isoforms measured in BK−/− mouse lumbosacral spinal cord (Table 3).
The normalization of evoked vesicle release in BK−/− NMJs by 3,4-DAP suggested administration of 3,4-DAP might normalize force production. To test this possibility, we performed nerve-stimulated force recordings with and without an intraperitoneal injection of 8 mg/kg 3,4-DAP (Fig. 5; Mori et al., 2012). After administration of 3,4-DAP, the force of the BK−/− nerve-stimulated twitch (Fig. 5 A) and tetanic contraction at 60 Hz (Fig. 5 B) dramatically increased. The peak twitch force of BK−/− muscle in the presence of 3,4-DAP (7.85 ± 0.51 N/g) was significantly higher than in the absence of 3,4-DAP (4.00 ± 0.40 N/g; P = 0.003), but not significantly different than WT in the presence (6.87 ± 0.99 N/g; P = 0.709) or absence (6.82 ± 0.11; P = 0.712) of 3,4-DAP (one-way ANOVA, Tukey correction). Similarly, at 60 Hz stimulation, the peak force of BK−/− muscle in the presence of 3,4-DAP (19.12 ± 0.39 N/g) was significantly higher than in the absence of 3,4-DAP (10.11 ± 1.19 N/g; P = 1.16 × 10−4), but not significantly different than WT in the presence (14.95 ± 1.28 N/g; P = 0.526) or absence (16.86 ± 0.45; P = 0.070) of 3,4-DAP (one-way ANOVA, Tukey correction). As shown in Fig. 5 C, 3,4-DAP restored BK−/− muscle force generation throughout the full force–frequency relationship (0.3–80 Hz).
Nerve stimulation in BK−/− mice led to significantly lower force production than in WT mice. However, force production via direct stimulation of muscle was normal, consistent with a defect in neuromuscular transmission. Voltage-clamp studies revealed reduced vesicle release at BK−/− NMJs, which appeared to be due to a reduction in Ca2+ entry and subsequent decrease in the probability of vesicle release. The reduction in evoked vesicle release could be overcome by blocking Kv channels with 3,4-DAP to prolong nerve action potentials. Intraperitoneal injection of 3,4-DAP also normalized force production in vivo. Our study suggests that BK channels play a critical role in peripheral motor function such that mice lacking BK channels have reduced neuromuscular transmission that results in weakness.
Weakness in BK−/− mice is due to reduction of evoked acetylcholine release at the NMJ
BK−/− mice exhibit significant motor dysfunction, including problems with balance and gait, as well as weak grip (Meredith et al., 2004; Sausbier et al., 2004; Chen et al., 2010; Typlt et al., 2013). These deficits highlight the importance of BK channels in the normal function of the motor system. However, given the widespread expression of BK channels in the nervous system, it is difficult to ascribe motor dysfunction to dysfunction of specific neurons. There is evidence that dysfunction of cerebellar circuits contributes to ataxia caused by knockout of BK channels (Sausbier et al., 2004; Chen et al., 2010). However, dysfunction of the cerebellum does not cause weakness; therefore, there must be dysfunction of other parts of the motor system. Because the reduction in force following nerve stimulation recorded here is close to the magnitude of reduction in grip strength previously reported in BK−/− mice (Typlt et al., 2013), we posited that dysfunction of the NMJ is the primary contributor to weakness in BK−/− mice.
Our findings raise the possibility that motor dysfunction in patients with BK channel mutations (Bailey et al., 2019) may be due in part to a presynaptic problem causing the failure of neuromuscular transmission. Lambert–Eaton myasthenic syndrome is another disorder where weakness is due to a reduction of presynaptic vesicle release (Elmqvist and Lambert, 1968; Engisch et al., 1999). Lambert–Eaton myasthenic syndrome responds well to treatment with 3,4-DAP to increase presynaptic Ca entry (Newsom-Davis, 2003; Sedehizadeh et al., 2012). As 3,4-DAP normalized force in BK null mice, it may be worth trying in patients with weakness in the setting of BK channel mutations.
The role of BK channels in function of the mouse NMJ
At the NMJ, it is proposed that Ca2+ current through Cav2.1 (P/Q) channels helps to activate BK channels, which shorten action potential duration to limit release (Robitaille and Charlton, 1992; Robitaille et al., 1993; Katz et al., 1997; Protti and Uchitel, 1997; Ford and Davis, 2014; Dittrich et al., 2018). One would thus expect block/loss of BK channels to increase release by widening the presynaptic action potential. In agreement with this expectation, increased release following block of BK channels has been reported at the mouse NMJ (Vatanpour and Harvey, 1995; Flink and Atchison, 2003).
Our results with blockers of BK channels differ from the previous studies of BK channel function at the mouse NMJ. Varied results in the field include a study which found that the application of 300 nM charybdotoxin or 100 nM iberiotoxin caused a doubling of quantal content (Vatanpour and Harvey, 1995) and another which found that application of 150 nM iberiotoxin caused a doubling of the endplate potential (Flink and Atchison, 2003). There are some experimental differences between our studies and the previous studies, but none of them can easily explain the differing results. One study was performed using low extracellular Ca2+ to eliminate twitch (Vatanpour and Harvey, 1995), while the other crushed muscle fibers to eliminate twitch (Flink and Atchison, 2003). We found that blockers of BK channels had no effect on release when extracellular Ca2+ was decreased. Consequently, the discrepancy between results cannot be explained by differences in the level of extracellular Ca2+.
We recorded using a preparation that is as close to physiological as possible, normal extracellular Ca2+ with intact muscle fibers, in which twitch was prevented with µ-conotoxin to block muscle Na+ channels. Different studies also used different muscles. Moreover, the previous studies did not perform voltage clamp; as a result, their measures of changes in muscle membrane potential could be complicated by a change in muscle membrane properties after block of BK channels (Khedraki et al., 2017; Burke et al., 2018). Use of voltage clamp avoids this technical issue. We used three toxins, at higher doses than used previously, and found no change in evoked release. In some cases, toxins were purchased from more than one supplier to minimize the possibility that lack of effect was due to the purchase of ineffective toxin. We conclude that K+ current through BK channels has little to no effect on the function of the mature mouse NMJ in physiological conditions.
Despite the lack of effect of block of BK channels, knocking out BK channels had a dramatic effect on function of the NMJ. One possible explanation for this is that the defect in NMJ function in BK−/− mice arises from the method used to generate deletion of the channel. In the process of removing exon 1 to generate BK−/− mice, a neomycin-resistance cassette was retained in the BK−/− allele (Meredith et al., 2004). Retention of the PGK-neomycin (neomycin phosphotransferase gene with a phosphoglycerate kinase I promoter) can alter the expression of nearby genes (Olson et al., 1996; Pham et al., 1996; Scacheri et al., 2001). It is thus possible that the decreased release of acetylcholine reported here is a consequence of altered gene expression secondary to the retained neomycin gene. We do not favor this interpretation as two previous studies in Drosophila, which generated knockouts of the BK gene (slopoke) using different methods, found a similar reduction in neurotransmitter release at the NMJ (Warbington et al., 1996; Lee et al., 2008).
Another possibility is that there is a developmental effect leading to up-regulation of voltage-gated K+ (Kv) channels, as has been reported following knockout of BK channels in Drosophila (Lee et al., 2008, 2014). Up-regulation of Kv channels might shorten the presynaptic action potential to reduce Ca2+ entry such that the probability of release is reduced (Dittrich et al., 2018). While we did not find evidence supporting up-regulation of Kv channels in the spinal cord, our study of Kv channels was not exhaustive, and it remains possible that other Kv channels not assayed are up-regulated. It is also possible that there is a developmental effect independent of up-regulation of Kv channels.
Another way in which knockout, but not block, of BK channels might reduce release is through modulation of presynaptic Ca2+ channels or the coupling of Ca2+ channels with synaptic vesicles. BK channels have been found to colocalize with presynaptic Ca2+ channels at the NMJ (Robitaille et al., 1993) and have recently been shown to modulate P/Q (Cav2.1) Ca2+ currents through direct interaction (Dolphin, 2018; Zhang et al., 2018). P/Q-type Ca2+ channels play a central role in triggering vesicle release at the mouse NMJ (Uchitel et al., 1992; Hong and Chang, 1995; Wang et al., 2004). The modulation could occur via changes in either the number of channels present or the functional properties of the channels.
Our data are the first we are aware of to directly demonstrate that a defect in synaptic transmission in BK−/− mice does not arise from absence of BK current per se at the synapse. Instead, our data suggest that the BK channel plays a central role in development of the NMJ via modulation of presynaptic Ca2+ current or the effectiveness of Ca2+ in triggering release. In the absence of functional BK channels, vesicle release is reduced enough in vivo to cause failure of neuromuscular transmission and weakness.
Christopher J. Lingle served as editor.
We thank Dan Halm for helpful discussions and Andrea Meredith for providing the initial breeding pairs of BK−/− mice.
This work was supported by National Institutes of Heath grant AR074985 (M.M. Rich) and National Institute of Neurological Disorders and Stroke grant R15NS099850 (A.A. Voss).
The authors declare no competing financial interests.
Author contributions: X. Wang, S.R.A. Burke, and R.J. Talmadge performed experiments, analyzed and interpreted data, and helped write the manuscript. A.A. Voss and M.M. Rich designed experiments and wrote the manuscript.