Voltage-gated Na+ (NaV) channels comprise a macromolecular complex whose components tailor channel function. Key components are the non-covalently bound β1 and β3 subunits that regulate channel gating, expression, and pharmacology. Here, we probe the molecular basis of this regulation by applying voltage clamp fluorometry to measure how the β subunits affect the conformational dynamics of the cardiac NaV channel (NaV1.5) voltage-sensing domains (VSDs). The pore-forming NaV1.5 α subunit contains four domains (DI–DIV), each with a VSD. Our results show that β1 regulates NaV1.5 by modulating the DIV-VSD, whereas β3 alters channel kinetics mainly through DIII-VSD interaction. Introduction of a quenching tryptophan into the extracellular region of the β3 transmembrane segment inverted the DIII-VSD fluorescence. Additionally, a fluorophore tethered to β3 at the same position produced voltage-dependent fluorescence dynamics strongly resembling those of the DIII-VSD. Together, these results provide compelling evidence that β3 binds proximally to the DIII-VSD. Molecular-level differences in β1 and β3 interaction with the α subunit lead to distinct activation and inactivation recovery kinetics, significantly affecting NaV channel regulation of cell excitability.
In electrically excitable organs, such as the heart, brain, and skeletal muscle, voltage-gated Na+ (NaV) channels cause the initiation and propagation of action potentials by conducting a large and rapid inward Na+ flux. Within the cells of these tissues, NaV channels form macromolecular signaling complexes (Abriel, 2010) whose parts work in concert to regulate channel function. The NaV β subunit members of this complex have been shown to regulate cell adhesion (Isom et al., 1995; Malhotra et al., 2000; Yu et al., 2003) and signaling in addition to affecting channel density (Calhoun and Isom, 2014), gating kinetics (Fahmi et al., 2001; Watanabe et al., 2009; Calhoun and Isom, 2014), and pharmacology (Lenkowski et al., 2003; Uebachs et al., 2010). However, the mechanisms whereby the β subunits interact with the NaV channel α subunit to exert their influence on gating remain undiscovered.
Five types of NaV β subunits have been identified: β1, β2, β3, β4, and β1b (Hartshorne and Catterall, 1984; Messner and Catterall, 1985; Kazen-Gillespie et al., 2000; Morgan et al., 2000; Yu et al., 2003). β2 and β4 form covalent disulfide bonds with the α subunit (Isom et al., 1995; Yu et al., 2003), whereas β1 and β3 interact non-covalently (Isom et al., 1992; Morgan et al., 2000). With the exception of the β1b splice variant (Patino et al., 2011), the β subunits comprise a single transmembrane domain that is tethered to an extracellular Ig loop and a cytoplasmic C terminus (Calhoun and Isom, 2014). Very recently, the covalently bound β2 and β4 subunits were crystallized (Gilchrist et al., 2013; Das et al., 2016), and a crucial disulfide bond formed by 55Cys in β2 and 910Cys in the DII pore loop was identified (Das et al., 2016). However, 910Cys is not present in NaV1.5, and instead the homologous position is 868Leu.
The β subunits are widely expressed in many tissues, including the central and peripheral nervous system, the heart, and skeletal muscle (Calhoun and Isom, 2014). Despite the sequence homology between non-covalently associated β1 and β3 subunits, their expression profile across organs differs. For instance, β1, but not β3, is highly expressed in skeletal muscles (The Human Protein Atlas). Intriguingly, even in the same organ, β subunit localization can differ (Fahmi et al., 2001; Calhoun and Isom, 2014; Yuan et al., 2014). For example, the β1 and β3 subunits have been shown to differentially express in the atria and ventricles (Fahmi et al., 2001; Watanabe et al., 2009; Yuan et al., 2014), suggesting that they may specifically tailor NaV channel function according to cell type. Moreover, β1 and β3 also have a varied temporal expression profile during heart development. β1 expression has been shown to increase (Domínguez et al., 2005), whereas β3 has been shown to decrease through embryonic development (Okata et al., 2016). The dynamic expression patterns of β1 and β3 suggest that these two subunits play distinct roles in the regulation of NaV channel function and the action potential.
The pore-forming NaV channel α subunit is composed of four homologous domains (DI–DIV) connected by cytoplasmic linkers (Gellens et al., 1992). Each domain is formed by six α helical transmembrane segments (S1–S6). The fourth segments (S4) contain multiple positively charged residues that move across the membrane in response to changes in membrane potential. S4, together with S1–S3, form the voltage-sensing domains (VSDs) and are coupled to the S5 and S6, which form the channel pore. Upon membrane depolarization, the S4 segments within the VSDs of DI–DIII are propelled outward to open the channel within a millisecond; this is known as channel activation (Chanda and Bezanilla, 2002). Shortly thereafter, channels rapidly close—a process termed “fast inactivation” that is mediated by the intracellular DIII–DIV linker and the DIV-VSD (West et al., 1992). Both activation and inactivation gating have been previously shown to be modulated by the β1 and β3 subunits (Morgan et al., 2000; Fahmi et al., 2001; Watanabe et al., 2009; Calhoun and Isom, 2014).
Much mechanistic insight into NaV channel gating has been recently provided by applying the voltage clamp fluorometry (VCF) protocol, which is used to fluorescently track VSD conformation and correlate VSD kinetics with ionic current gating. For many years, this protocol has been applied to study the skeletal muscle isoform NaV1.4, and it has provided great insight into the VSD roles in determining activation and inactivation gating kinetics (Cha et al., 1999; Chanda and Bezanilla, 2002; Silva and Goldstein, 2013a,b), the mechanisms of local anesthetic regulation of the VSDs (Muroi and Chanda, 2009; Arcisio-Miranda et al., 2010), and details of how toxins pathologically affect VSD activation (Campos et al., 2007, 2008). We have recently broadened this approach by developing VCF constructs to track VSD conformations of all four domains in the cardiac paralog, NaV1.5 (Varga et al., 2015; Zhu et al., 2016), whose ionic current modulation by the β subunit in oocytes mirrors the mammalian cell phenotype.
We hypothesized that the non-covalently bound β1 and β3 subunits would modulate NaV1.5 ionic current kinetics by altering the activation of one or more VSDs. In this study, we test this hypothesis by applying VCF to observe the β subunit effects on the VSD of each NaV1.5 domain.
Materials and methods
cDNA encoding the human NaV β3 (UniProtKB/Swiss-Prot under accession no. Q9NY72) subunit was custom synthesized by Life Technologies and inserted into the pBSTA plasmid. cRNAs for the human β1 subunit (UniProtKB/Swiss-Prot under accession no. Q07699.1) and α subunit NaV1.5 (accession no. Q14524.1) were produced from the pBSTA and pMAX vectors, respectively. All mutagenesis was accomplished using the QuikChange II site-directed mutagenesis kit (Agilent), with primers from Sigma-Aldrich. Multiple colonies were picked, and plasmids were isolated using the NucleoSpin plasmid miniprep kit (Macherey-Nagel). After samples were confirmed with sequencing (Genewiz), a single clone was selected for a Midiprep preparation (NucleoBond Xtra Midi; Macherey-Nagel). Each plasmid was then linearized with the NotI or EcoRI restriction enzyme and purified with the NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel). Finally, capped mRNA was synthesized in vitro using the mMESSAGE mMACHINE T7 Transcription Kit (Life Technologies), purified via phenol–chloroform extraction, and reconstituted to a concentration of ∼1 µg/µl.
Cut-open oocyte recording
mRNAs for the human α subunit NaV1.5 and β1 or β3 subunits were injected at a 3:1 molar ratio (50–56 ng per cell total) into Xenopus oocytes. Oocytes were then incubated at 18°C in ND93 solution (93 NaCl mM, 5 KCl mM, 1.8 CaCl2 mM, 1 MgCl2 mM, 5 HEPES mM, 2.5 Na pyruvate mM, and 1% penicillin–streptomycin, pH 7.4). 3–7 d after injection, cut-open recordings (Stefani and Bezanilla, 1998; Rudokas et al., 2014) were performed using a cut-open amplifier (CA-1B; Dagan Corporation) coupled to an A/D converter (Digidata 1440; Molecular Devices). Clampex software (v10; Molecular Devices) was used for data acquisition. During recording, the temperature was maintained at 19°C with a controller (HCC-100A; Dagan Corporation). The internal recording solution was composed of 105 NMG-Mes mM, 10 Na-Mes mM, 20 HEPES mM, and 2 EGTA mM, at a pH level of 7.4, and the external solution was composed of 25 NMG-Mes mM, 90 Na-Mes mM, 20 HEPES mM, and 2 Ca-Mes2 mM, at a pH level of 7.4.
Before recording, the membrane capacitance compensation and P/–8 leak subtraction were applied. The ionic currents were recorded using the standard I-V protocol. From a holding potential of −120 mV, cells were stepped to a 100-ms prepulse of −120 mV and then stepped to test potentials ranging from −120 to 60 mV with a 10-mV increment, preceded by a 100-ms postpulse of −120 mV. For steady-state inactivation (SSI), cells were held at test potential for 200 ms; availability was then tested using a depolarizing pulse of −20 mV. Gating currents were recorded during test pulses from −150 to 50 mV from a holding potential of −120 mV. Capacitance and leak were compensated by P/4 leak subtraction with a subsweep potential of 40 mV. Gating charge–voltage (Q-V) curves were constructed by integrating gating currents over 7 ms after the voltage step.
Voltage clamp fluorometry
Before recording, oocytes were labeled with 10 µmol/L methanethiosulfonate-carboxytetramethylrhodamine (MTS-TAMRA; Santa Cruz Biotechnology) in a depolarizing solution (in mM: 110 KCl, 1.5 MgCl2, 0.8 CaCl2, 0.2 EDTA, and 10 HEPES, pH 7.1) for 30 min on ice. Fluorescence data were collected simultaneously with ionic current on a custom rig (Varga et al., 2015), combining the cut-open voltage clamp and an epifluorescence upright microscope (FN1; Nikon), using a 40× water-immersion objective with 0.8 NA (CFI Plan Fluor; Nikon). A green, high-powered LED (Luminus; PT-121) was used for illumination, controlled by a driver (Lumina Power; LDPC-30-6-24VDC) by Clampex software. The emission light was measured with a photodiode (PIN-040A; United Detector Technology) mounted on the microscope epifluorescence port. The photocurrents generated by the photodiode were then amplified by a patch clamp amplifier (Axopatch-200A; Molecular Devices). Each fluorescence trace is a mean of 7–10 fluorescence recordings of the same cell.
Data analyses were performed using Clampfit (v10; Molecular Devices), MATLAB (R2012a; MATLAB), and Excel (Microsoft). For fluorescence data, signals were low-pass filtered at 1 kHz offline before analysis. To correct for photobleaching, the baseline fluorescence trace, which has no change in voltage, was fit and subtracted from the traces recorded when the voltage protocol was applied.
Steady-state voltage dependence curves (G-V, fluorescence against voltage [F-V], SSI) were quantified by fitting a Boltzmann function: y = 1/(1 + exp[(V − V1/2)/k]). Sample sizes were chosen so that the standard error of mean was less than 0.1 for each data point, and a minimum sample size of three was determined to calculate the SD. Each data point shown reflects n = 3 or more from two or more batches of oocytes. Statistics for comparison between different constructs were performed using an independent t test (Microsoft Excel). The ± symbols in the text and table and the error bars in the figures represent the SEMs.
Online supplemental material
The supplemental material contains data of channel currents properties that are not depicted in the main figures and other control data. Figs. S1 and S2 show channel activation and inactivation with or without β1 and β3 for NaV1.5 expressed in HEK 293T cells and for four VCF constructs. Fig. S3 shows the voltage dependence of fluorescence and gating charges for the decoupling mutations, A1330W and N1759A. Fig. S4 shows the fluorescence data from all four domains with S156W β1 or S155W β3. Fig. S5 shows DIII and DIV fluorescence deactivation kinetics comparisons for NaV1.5 expressed with β1/β3 chimeras.
β1 regulates channel inactivation by altering voltage-dependent DIV-VSD transitions
We coexpressed the human β1 subunit with the pore-forming NaV1.5 α subunit in Xenopus oocytes by coinjecting β1 and α subunit mRNA at a molar ratio of 3:1. β1 coexpression had no significant effect on the voltage dependence of WT channel activation, as shown by the conductance-voltage (G-V) curve (Fig. 1 b and Table 1) but caused a depolarizing shift in the channel steady-state inactivation (SSI) curve compared with WT alone (ΔV1/2 = 12.2 ± 1.4 mV, P = 0.02; Fig. 1 b). The right-shifted SSI curve implies that more channels are available to open at higher potentials. Moreover, β1 further increased channel opening by accelerating channel recovery from inactivation (Fig. 1 d). To ensure that the changes in the β1 regulation mechanism that we observed were consistent across different expression systems, we also used identical protocols to assess β1 effects on NaV1.5 currents in HEK 293T cells and observed similar behavior (Fig. S1 a). The β1-induced depolarization of SSI we observed is also consistent with previous results in HEK 293 and HEK 293T cells (An et al., 1998; Malhotra et al., 2001; Maltsev et al., 2009).
We investigated how β1 modulates inactivation by first measuring gating currents, which reflect charge translocation of all four VSDs. To be able to measure the gating current for the NaV1.5 channel, we used the WT LFS construct, which contains C373Y mutation that increases channel sensitivity to TTX, and the Y1977A mutation, which prevents ubiquitination of the channels to increase expression (Varga et al., 2015). Comparison between the gating charge–voltage dependence (Q-V) of WT LFS channels coexpressed with and without the β1 subunit (Fig. 1 e) revealed that β1 caused a depolarizing shift in the Q-V curve at negative potentials, resulting in a steeper Q-V relationship (Δk = −10.1 ± 4.2 mV, P = 0.04). This result suggests that in the presence of the β1 subunit, one or more of the VSDs requires higher potentials to activate. To identify which VSD was affected, NaV1.5 VCF constructs were coexpressed with the β1 subunit. We have previously shown that MTS-TAMRA–labeled NaV1.5 channels activate and inactivate similarly to WT channels (Varga et al., 2015). Coexpression of the β1 subunit with the VCF constructs caused a shift in the SSI curves that is similar to the shift caused by β1 in WT channels (Fig. S1 b). The voltage dependence of activation of each VSD can be described by plotting the steady-state fluorescence against voltage (F-V) curve. In comparison to α alone, the β1 subunit did not significantly alter the DI, DII, or DIII F-V curves (Fig. 1, f–h), but induced a strong depolarizing shift in the DIV F-V curve (ΔV1/2 = 31.3 ± 1.7 mV, P = 0.02; Fig. 1 i). Thus, in the presence of the β1 subunit, the DIV-VSD requires higher potentials to accomplish its activation transition, consistent with the gating charge shift (Fig. 1 e).
Previously, DIV-VSD activation was shown to be more closely linked to NaV channel inactivation than activation (Cha et al., 1999; Capes et al., 2013). Specifically, the DIV-VSD was observed to be immobilized by fast inactivation (Cha et al., 1999), and DIV-VSD activation was shown to be the rate-limiting step for fast inactivation (Capes et al., 2013). Hence, changes in the voltage dependence of DIV-VSD activation or DIV-VSD kinetics would be expected to cause correlated changes in channel SSI or inactivation kinetics. Notably, DIV-VSD deactivation kinetics are also faster with β1 (t100–10% = 4.5 ± 0.6 ms at −160 mV after 0-mV pulse) compared with α alone (t100–10% = 13.2 ± 0.4 ms at −160 mV after 0-mV pulse, P = 0.0003). Thus, our results imply that the β1 subunit regulates inactivation by altering DIV-VSD transitions. This finding is consistent with the results of previous studies suggesting that β1 binds to the C terminus of NaV1.1 (Spampanato et al., 2004) and the S5–S6 linker of DIV of NaV1.4 (Makita et al., 1996). Our results build on these previous findings by connecting VSD regulation to altered inactivation kinetics.
Even though β1 does not affect the voltage dependence of DIII-VSD activation, comparison of DIII-VSD deactivation kinetics in the presence of β1 shows that DIII-VSD recovery to the resting position upon repolarization is faster and more complete (t100–10% = 16.5 ± 1.6 ms at 0 mV) in contrast to α alone (t100–10% = 23.9 ± 2.3 ms at 0 mV, P = 0.05; Fig. 1 h, right). In previous studies, fast inactivation was shown to immobilize the gating charge displaced by DIII and DIV (Armstrong and Bezanilla, 1977; Cha et al., 1999), particularly DIII-VSD (Sheets and Hanck, 2005; Varga et al., 2015). Our results suggest that β1 allows the DIII and DIV VSDs to recover to the resting state more quickly. Given the link to inactivation, this more rapid recovery of the VSDs will allow channels to recover more quickly from inactivation (Fig. 1 d) and become available for excitation in a shorter amount of time.
β3 alters channel activation and inactivation by modulating DIII and DIV VSD kinetics
We coexpressed the β3 subunit with NaV1.5 using the same protocols that were used for β1 (Fig. 2 a). As with the β1 subunit, β3 had no apparent effect on the voltage dependence of channel activation (G-V; Fig. 2 b), but slowed ionic current activation and inactivation kinetics (Fig. 2 c). It also caused a depolarizing shift (ΔV1/2 = 8.7 ± 1.5 mV, P = 0.02) in SSI (Fig. 2 b and Table 1), implying that β3 expression increases NaV1.5 channel availability at higher potentials. A similar β3-induced SSI shift was also present in HEK cells recorded with identical protocols (Fig. S2 a). Unlike β1, β3 does not significantly alter channel recovery kinetics (Fig. 2 d). When we coexpressed β3 with the four VCF constructs, the gating effects of β3 were preserved (Fig. S2 b), except with the DII LFS construct, where the shift in SSI induced by β3 is less pronounced. Our observations of the ionic current changes induced by β3 coexpression are consistent with the gating effects shown previously in oocytes (Fahmi et al., 2001) and the scn3b knockout mouse phenotype (Hakim et al., 2008).
Like β1, β3 caused a depolarizing shift in the Q-V curve at negative potentials and a steeper Q-V relationship (Δk = 8.6 ± 3.0 mV, P = 0.05), showing that β3 also alters the voltage dependence of VSD activation. Considering the homology between the β1 and β3 subunits, the similar Q-V curves are not surprising. However, comparison of the F-V curves of α alone and α with β3 shows that β3 induces a depolarizing shift in the DIII F-V curve (DIII F-V: ΔV1/2 = 20.7 ± 3.9 mV, P = 0.01) in addition to its depolarizing effect on the DIV F-V curve (DIV F-V: ΔV1/2 = 25.0 ± 7.7 mV, P = 0.01; Fig. 2, h and i; and Table 1), causing both the DIII and DIV VSDs to activate at higher potentials. The DIV F-V depolarizing shift occurs over the same potential range as the shift in SSI, consistent with the findings that DIV-VSD activation strongly correlates with channel inactivation and with the aforementioned experiments with β1.
Channel opening is known to be regulated by DIII-VSD activation (Muroi et al., 2010; Wang et al., 2016). Yet, β3 induced depolarization of DIII-VSD activation without affecting the channel voltage dependence of activation (G-V), which may be due to DIII-VSD activation at very negative potentials in the NaV1.5 paralog. Still, β3 slowed ionic current activation kinetics (α alone: dI/dtmax = 1.6 ± 0.1 ms−1; α + β3: dI/dtmax = 1.1 ± 0.1 ms−1, P = 0.04; Fig. 2 c). Slower inactivation rates can also result in slower activation kinetics when normalized currents are compared because channel activation and inactivation are tightly coupled (Aldrich et al., 1983). Thus, the slowed activation kinetics we observed with β3 could alternatively be caused by slowed inactivation kinetics. In contrast to β1, β3 only accelerates DIII-VSD deactivation (α + β3: t100–10% = 9.2 ± 1.3 ms; α alone: t100–10% = 23.9 ± 2.3 ms, P = 0.005), but not DIV-VSD deactivation (see Table 4). In the NaV1.5 channel, the DIII-VSD activates at very negative potentials (∼160 mV). Thus, it is a technical challenge to acquire the negative baseline of the DIII F-V curve for the more hyperpolarized shifted constructs (α alone and α + β1). Despite this limitation, we expect that the hyperpolarized shifted constructs would have more negative V1/2 if we were able to record to the baseline, suggesting that the DIII depolarizing shift induced by β3 is even larger than reported.
Despite being highly homologous to β1, β3 is unique in altering the VSD transitions of both DIII and DIV. The DIV-VSD effects induced by β3 are similar to those induced by β1, causing a depolarizing SSI shift. The β3 effect on the DIII-VSD, which shifts DIII-VSD’s activation to higher potentials, slows ionic current activation and inactivation kinetics. Two α–β3 interaction mechanisms could explain the changes in the VSD movements that we observed. One possibility is that β3 can interact with both DIII and DIV. A second plausible mechanism is that β3 mainly interacts with the DIII-VSD, which can allosterically modify the adjacent DIV-VSD activation. In the following sections, results from β1/β3 chimera and α–β3 quencher fluorophore pair experiments support the latter mechanism.
High expression of β3 separates DIII VSD activation into two steps
To ensure that the VSD alterations we observed were truly caused by the expression of β subunits and that the amount of β subunits expressed on the membrane saturated the modulation effects of NaV1.5 channels, we tested different expression levels of β subunits. We altered β subunit expression levels by injecting mRNAs encoding α and β subunits at different molar ratios, observing their effects on ionic current and VSD activation.
For the β1 subunit, as we increased the mRNA molar ratio from 1:1 to 1:2, the DIV F-V curve shifted to more depolarized potentials (1:1 α:β1: V1/2 = −70.1 ± 5.2 mV, 1:2 α:β1: V1/2 = −56.8 ± 5.0 mV). Further, when the α:β1 mRNA molar ratio was increased to 1:4, the DIV F-V curve overlapped with the F-V curve of a 1:2 α:β1 mRNA molar ratio (Fig. 3 a), suggesting that β1 modulation of DIV-VSD saturated at a 1:2 α:β1 ratio. Consistently, the β1 alteration of channel SSI followed a similar saturation pattern (Fig. 3 b). This result further supports the idea that β1 regulates channel inactivation by altering DIV-VSD activation, an effect that saturates at a 1:2 ratio.
For β3, the saturation behavior was more complex. When we increased the mRNA molar ratio from 1:1 to 1:2, the DIII F-V curve shifted to depolarized potentials (1:1 α:β3: V1/2 = −108.6 ± 5.6 mV; 1:2 α:β3: V1/2 = −97.9 ± 2.6 mV; Fig. 3 c). Intriguingly, when the molar ratio of α:β3 was increased to 1:4 or higher, the DIII F-V curve started to exhibit two components that could no longer be fit with a single Boltzmann function (Fig. 3 c). The curve was well fit with two Boltzmann curves, one at very negative potentials (−180 to −80 mV), and the other within the channel activation voltage range (−80 to 20 mV). Correspondingly, the DIII fluorescence kinetics also followed two steps, which also were not well fit with a single exponential. The first rapid transition occurred within 2 ms after depolarization, denoted by F1, followed by a very slow component, denoted by F2, over a time period of 60 ms (Fig. 3 g).
The separation of two components in DIII-VSD activation caused unusual recovery from inactivation, where the peak current magnitude during the test pulse was larger than that during the control pulse after recovery times of 10 to 300 ms (Fig. 3, e and f). After 500- to 1,000-ms recovery at −120 mV, the peak current during the test pulse returned to the magnitude of the control pulse. Typically, Na+ currents exhibit monotonic behavior during this protocol.
We assessed the relationship between the two DIII-VSD activation components and channel recovery from inactivation by aligning current during the first control pulse with that of the second test pulse after 10- or 1,000-ms recovery (Fig. 3 i, bottom left, top right). For channels expressed without the β subunit, current during the test pulse after 10-ms recovery activated at the same rate as the control pulse (Fig. 3 i, top left). For channels coexpressed with the β3 subunit at a 1:4 molar ratio, current during the test pulse after 10-ms recovery activated more quickly in comparison with the control pulse (Fig. 3 i, bottom left). In contrast, the current during the test pulse after 1,000-ms recovery rose at the same rate as that of the control pulse (Fig. 3 i, top right). Faster channel activation kinetics can significantly increase peak current. High β3 expression causes faster activation kinetics during a test pulse that follows a short recovery interval (10–300 ms), resulting in peak current that exceeds the control pulse current.
To account for this behavior, we first suppose that the DIII-VSD activates in two steps from resting (R) to intermediate activated (A1) and activated (A2) states (Fig. 3 h). We then assume that the transition from R to A1 is fast and is described by the F1 component, whereas the transition from A1 to A2 is slow, as shown by the F2 component. In this model, pore opening is facilitated by the transition of the DIII-VSD to the A1 state and further encouraged by entry into the A2 state. During the first 200-ms pulse, most DIII-VSDs are brought to A2. Because the transition from A2 to A1 is slow, when channels were given 10–300 ms to recover at −120 mV, the time was too short for DIII VSD to recover to A1, resulting in most of the DIII-VSDs being trapped in the A2 state. As most of the DIII-VSDs were still in the A2 state and it greatly facilitates pore opening, channel activation was faster for the second pulse. If this scheme is correct, we would predict that if we only allow the DIII-VSD to enter A1 by applying a short 2-ms depolarizing pulse as the control pulse, the second pulse will not have faster rising kinetics compared with the control pulse. Indeed, comparison of the control and test pulses shows that both pulses completely overlap (Fig. 3 i, bottom right).
It is unlikely that the physiological assembly of α–β3 will reach a ratio high enough to separate DIII-VSD movement into two components (Yuan et al., 2014). It is possible that overexpression of β3 will force some of the β3 subunits into a secondary low-affinity binding site. Consequently, if β3 is locally expressed at very high levels, this group of cells will have a relatively short refractory period and become particularly excitable because of the unique channel recovery behavior.
β1 and β3 do not regulate VSD activation from the NaV1.5 pore via the S4–S5 linkers
By probing the VSD dynamics, we discovered that β1 and β3 modulate channel current by altering DIII and DIV VSD activation. However, the mechanism by which the β1 and β3 subunits interact with the NaV channel to alter VSD activation remains unclear. The recent cryo-electron microscopy (cryo-EM) structure of the eukaryotic NaV channel shows that the pore loops of the NaV channel have bulky extracellular structures, making them good candidates for β subunit binding (Shen et al., 2017). To test if the β1 and β3 subunits allosterically modulate the VSDs by interacting with the DIII and DIV pore domains, we assessed β1 and β3’s effects on mutant channels where DIII or DIV VSD is decoupled from the pore. The interaction between the S4–S5 linker and the S6 is known to be essential for canonical coupling between the VSD and the pore of each domain. We used mutations that have previously been found to disrupt this type of coupling.
Residue N1765, which is on the S6 of DIV, has previously been shown to be essential for coupling the DIV-VSD to the pore (Sheets et al., 2015). Mutating N1765 to alanine (A) abolishes most of the ionic current (Fig. 4 a and Table 2) without affecting DIV-VSD movement (Fig. S3 a) and gating currents (Fig. S3 c), suggesting that the DIV-VSD is decoupled from the pore. Coexpressing β1 with the N1765A channel still depolarized the Q-V curve (Fig. S3 c) and the DIV F-V curve (Fig. 4 a), similar to the β1 effect on WT channels. If β1 interacts with the DIV pore domain to allosterically modulate the DIV-VSD through the S4–S5 linker, decoupling of the DIV-VSD from the DIV pore domain should abolish most of the β1 effect on the DIV-VSD. Instead, we found that the DIV-VSD of the N1765A is still modulated by β1, suggesting that β1 does not regulate the DIV-VSD via pore coupling through the DIV S4–S5 linker but instead interacts with the DIV-VSD directly or via an alternative pore–VSD coupling mechanism.
Because β3 was shown to alter DIV-VSD activation (Fig. 2), we also tested the β3 effect on the N1765A channel. Like β1, β3 still causes depolarizing shifts in the DIV F-V curve (Fig. 4 c) and the voltage dependence of gating charges (Q-V; Fig. S3 d) of the N1765A channel, suggesting that β3 does not interact with the DIV pore domain to regulate DIV-VSD activation via the S4–S5 linker. In addition to the DIV effect, we also showed that the DIII-VSD is significantly affected by β3. We assessed the β3 effects on channels that contain a mutation that decouples the DIII pore from its VSD. A1149, located on the DIII S4–S5 linker of NaV1.4, is one of the key residues on the gating interface (Muroi et al., 2010). Mutating A1149 to W in the NaV1.4 stabilizes the activated DIII-VSD, but not the pore, suggesting that the coupling between the DIII-VSD and the DIII pore is reduced (Muroi et al., 2010). The homologous residue in the NaV1.5 isoform is A1330. Consistent with previous studies, A1330W greatly hyperpolarized the DIII F-V curve (Fig. S3 b) compared with WT. However, ionic current activation (G-V) was not significantly affected (Fig. S3 b). When coexpressed with β3, the DIII F-V curve was still significantly depolarized (ΔV1/2 = 22.6 ± 6.7 mV, P = 0.03) compared with A1130W alone (Fig. 4 b and Table 2). This result suggests that β3 does not regulate DIII-VSD activation via S4–S5 linker coupling to the DIII pore domain. However, there are alternate means to couple the pore to the VSD, including the DIII–DIV linker, and these alternative mechanisms could possibly be in play.
Assessing β subunit localization
Because we observed that β1 and β3 modulate channel gating by affecting the DIII and DIV VSDs, we hypothesized that β1 and β3 are proximally located to these VSDs. To test this hypothesis, we introduced a fluorophore or a quenching tryptophan into the β subunit. This method was previously applied to other proteins, such as the T4 lysozyme (Mansoor et al., 2002, 2010) and the BK channel (Pantazis and Olcese, 2012), to map distances within proteins. We reasoned that if β1 or β3 resides near one of the channel’s VSDs, introducing a tryptophan residue to the top of the transmembrane segment of β1 or β3 would quench the fluorophore attached to the S3–S4 linker of that VSD.
We first introduced a tryptophan mutation to the extracellular region of the β1 transmembrane segment, S156W. Compared with WT β1, S156W β1 only slightly depolarizes the DI and DIV F-V curves, but none of the domains’ fluorescence signals were significantly quenched (Fig. S4). This result suggests that the S156 residue of β1 is not within detectable quenching distance to the S4s of any domain.
We then introduced a tryptophan mutation into the extracellular region of the β3 transmembrane segment, S155W. S155W β3 did not affect DI, DII, or DIV F-V curves or fluorescence compared with WT β3, but the fluorescence of the DIII-VSD was completely reversed (Fig. 5 a). In the DIII LFS construct, the DIII-VSD fluorescence signal moves downward upon membrane depolarization (Fig. 2 h). This reduction in fluorescence is consistent with local environment quenching of the fluorophore attached to the S3–S4 linker of DIII upon activation. In contrast, when S155W β3 is present, its tryptophan strongly quenches the fluorophore on the DIII S3–S4 linker when S4 is at resting position. When S4 activates, the fluorophore moves away from the tryptophan, resulting in an increase in fluorescence. This result shows that the β3 subunit resides very close to the DIII-VSD at a distance that is within the van der Waals contact distance (5–15 Å; Mansoor et al., 2002). Notably, the DIII fluorescence kinetics with S155W β3 are greatly slowed, and the F-V curve has similar voltage dependence (V1/2 = −67.2 ± 3.5 mV) as the second component of DIII F-V with high β3 expression (Fig. 3 c). Thus, the DIII fluorescence with S155W β3 tracks a slower component of the DIII-VSD that occurs at higher potentials, a component that is observed but not prominent with the LFS construct.
If β3 and the DIII-VSD are close in proximity, we would also expect to track DIII-VSD conformational changes by labeling the β3 subunit. To test this hypothesis, we introduced a cysteine into the extracellular β3 segment, S155C. We coexpressed S155C β3 with the WT NaV1.5 α subunit in Xenopus oocytes. We then labeled the S155C β3 with a fluorophore (MTS-TAMRA). A voltage-dependent fluorescence signal was detected (Fig. 5 b). Changes in the local environment of the fluorophore attached to β3 when DIII-VSD changed conformation likely produced this signal. The F-V curve of the WT α subunit with the labeled S155C β3 subunit is comparable to the F-V curve of the labeled DIII LFS α subunit with the WT β3 subunit (Fig. 5 b). This result suggests that the fluorescence signal generated by the labeled β3 subunit represents the conformational changes of the DIII-VSD.
Using the tryptophan-induced fluorophore quenching method (Mansoor et al., 2002, 2010; Pantazis and Olcese, 2012), we demonstrated β3’s proximity to the DIII-VSD. Given that β3 still affects the DIII-VSD that is decoupled from the pore by the A1330W mutation and the location of the β3 subunit, we conclude that β3 modulates the DIII-VSD by direct interaction. Taking advantage of β3 proximity to the DIII-VSD, we are now able to track the DIII-VSD conformation without directly labeling the α subunit.
β1 and β3 chimeras show that both the extracellular and transmembrane domains of β3 are essential for its interaction with the DIII-VSD
We observed that β1 and β3 have distinct interactions with channel VSDs, especially the DIII-VSD. In response, we sought to further understand which part of the β subunit is essential for these interactions. We created three chimera β1 and β3 subunits (Fig. 6 a): one with the β3 extracellular domain and β1 transmembrane and intracellular domain (β3-N β1-TMC), one with the β1 extracellular domain and β3 transmembrane and intracellular domain (β1-N β3-TMC), and one with both the extracellular and transmembrane domains of β3 and the β1 intracellular domain (β3-NTM β1-C). Comparison of the DIII F-V curves for all three chimeras illustrates that both the extracellular and transmembrane domains of β3 are necessary for its modulation of the DIII-VSD activation because only the chimera that contains both the extracellular and transmembrane domains of β3 (β3-NTM β1-C) caused a depolarizing shift in the DIII F-V curve (Fig. 6 d and Table 3) compared with α alone (ΔV1/2 = 22.9 ± 5.0 mV, P = 0.003), resembling the shift induced by the WT β3 subunit (ΔV1/2 = 20.7 ± 3.9 mV, P = 0.01). The other two β1/β3 chimeras caused no shift in the DIII F-V curve compared with α alone, resembling the β1 subunit effects (Fig. 6, b and c; and Table 3).
The DIV-VSD response to these β1/β3 chimeras is more difficult to interpret because both WT β1 and β3 cause comparable depolarizing shifts in the DIV F-V curve (Figs. 1 and 2). Notably, the β3-N β1-TMC chimera causes a larger depolarizing shift of the DIV-VSD (ΔV1/2 = 32.7 ± 1.2 mV, P = 0.001) than β1 or β3 (Fig. 6 e). Both β1-N β3-TMC and β3-NTM β1-C cause DIV F-V depolarization that is similar to that of WT β1 or β3 (Fig. 6, f and g; and Table 3). This result suggests that the interaction of the β3 extracellular domain with the channel can cause additional modulation of the DIV-VSD and induce an interaction mechanism that is distinct from the transmembrane and C terminus of the β1 subunit. Previously, we observed that the WT β1 subunit causes the DIV-VSD to deactivate more quickly (Fig. 1). Comparing the DIV-VSD deactivation kinetics of these chimeras, we note that both the β3-N β1-TMC and β3-NTM β1-C chimeras that contain the β1 C terminus resemble the fast DIV deactivation induced by WT β1 (β3-N β1-TMC: t100–10% = 3.9 ± 0.6 ms, P = 0.0002; β3-NTM β1-C: t100–10% = 3.7 ± 0.3 ms, P = 0.0004, compared with α alone; Fig. S5 and Table 4), suggesting that the β1 C terminus is important for speeding DIV-VSD deactivation upon membrane repolarization.
By monitoring DIII and DIV VSD conformations in the presence of β1/β3 chimeras, we found that the extracellular and transmembrane domains of the β3 subunit are essential for depolarizing DIII-VSD activation, which controls channel current activation kinetics. The C terminus of β1 is essential for speeding DIV-VSD deactivation kinetics upon membrane repolarization, which will affect channel recovery from inactivation.
β1 and β3 effects on the DIII and DIV VSDs are not independent
Both β1 and β3 have been shown to express in the ventricular myocardium (Fahmi et al., 2001; Olesen et al., 2011) to modify NaV1.5 gating in concert. To assess the molecular consequences when both β1 and β3 subunits are expressed, we coinjected mRNA of the α, β1, and β3 subunits at a molar ratio of 1:3:3 (Fig. 7 a). As with coexpression of β1 or β3, simultaneously coexpressing the channel with both causes a depolarizing shift in the SSI curve (Fig. 7 b) compared with the α subunit alone. The magnitude of this shift does not significantly differ from the shifts induced by β1 or β3 (Fig. 7 b), raising two possible mechanisms. One explanation is that only one of the β1 and β3 subunits can coassemble with the α subunit at a time, so that the shift is a mean of the shifts induced by either β1 or β3. The other possibility is that β1 and β3 binding to the α subunit is not exclusive, and there might be some cooperation and interaction between the β1 and β3 subunits that are bound to the same α subunit. The latter mechanism appears to be more likely, as we also observed a depolarizing shift in the G-V curve caused by coexpression with both the β1 and β3 subunits (ΔV1/2 = 7.2 ± 2.3 mV, P = 0.02; Fig. 7 b), which was not seen in α with only β1 or β3.
To gain insight into the potential interaction mechanisms between β1 and β3, we assessed the coexpression of both β1 and β3 on the activation of each VSD. Coexpression of β1 and β3 has minimal effects on DI and DII VSD activation, as no significant shift was observed in the DI and DII F-V curve (Fig. 7, c and d), consistent with the previous observation that β1 and β3 do not perturb DI or DII VSDs. Compared with α alone, coexpression with both β1 and β3 caused a depolarizing shift in the DIII-VSD (Fig. 7 e). The magnitude of this shift was greater than the linear addition of the shifts induced by β1 only and β3 only (dotted line), suggesting that when both β1 and β3 were present, they produced additional depolarization of DIII-VSD activation, corresponding to the depolarizing shift in channel activation. Thus, we infer that there must be cooperation between β1 and β3 that causes a greater effect on the DIII VSD when both are interacting with the channel.
In contrast, expressing β1 and β3 together caused a DIV F-V depolarizing shift that most closely resembles the shift produced by β1 or β3 only (Fig. 7 f) and that also corresponds to the SSI shift (Fig. 7 b). If β1 and β3 act independently, the DIV F-V shift should resemble the linear addition of β1- and β3-induced shifts (Fig. 7 f, dotted line). Thus, this result suggests that β1 and β3’s modulation of the DIV-VSD is not additive.
Together, these results show that β1 and β3 binding to the NaV1.5 channel is not exclusive, and when both β subunits are present, their cooperativity further depolarizes DIII-VSD activation, which leads to additional modifications of channel activation. The physiological impact of this result is limited because this experiment was conducted in the condition of overexpressing β subunits, which may cause additional binding that may not happen in native cells. More information regarding the stoichiometry of α, β1, and β3 in native cells is required to infer the physiological consequences of β1 and β3 subunit cooperativity.
The noncovalently bound NaV channel β1 and β3 subunits were first identified in 1985 (Messner and Catterall, 1985) and 2000 (Morgan et al., 2000). Despite recent findings showing that these subunits play a critical role in regulating neuronal and cardiac electrophysiology (Calhoun and Isom, 2014), the precise mechanisms that they use to modulate channel gating have not been described. In this study, we used VCF to test the hypothesis that the β1 and β3 subunits regulate NaV channel kinetics via the VSDs. We discovered that WT β1 subunit coexpression shifts DIV-VSD activation to depolarized potentials, consistent with the shift in SSI. β1 subunits also relieve the immobilization of the DIII and DIV VSDs by fast inactivation, which potentially contributes to the increased rate of channel recovery from inactivation induced by β1.
The WT β3 subunit also regulates channel inactivation with a corresponding shift in DIV-VSD activation. However, in contrast to β1, there is a prominent WT β3 interaction with the DIII-VSD. We believe that β3 modulation of the DIII-VSD is primary because we showed that the transmembrane domain of β3 is very close to the DIII S4 by tryptophan-induced fluorophore quenching. Moreover, β1/β3 chimeras mainly affect the DIII-VSD without changing DIV-VSD activation. Recently, we showed that DIII-VSD deactivation strongly correlates with channel recovery from inactivation, a phenomenon that is determined by the DIII S4–S5 linker’s interaction with the inactivation gate after depolarizing pulses of ∼100 ms (Hsu et al., 2017). Here, we observed that β3 does not affect channel recovery from inactivation even though it speeds DIII-VSD deactivation, suggesting that β3 possibly disrupts the interaction between the DIII S4–S5 linker and the inactivation gate, abolishing DIII-VSD regulation of recovery from inactivation.
Despite the β1 and β3 subunits being homologous, we demonstrate that they have distinct interactions with the NaV channel VSDs, resulting in different current kinetics and rates of inactivation recovery. Consistently, our results from the tryptophan-induced quenching experiment showed that β1 and β3 assemble with the channel at different locations. As β1 and β3 have very different spatial and temporal expression patterns in the heart (Domínguez et al., 2005; Okata et al., 2016), the molecular interactions that we have observed will significantly affect their regulation of tissue excitability. For regions that have higher β1 expression—for example, Purkinje fibers in the heart (Domínguez et al., 2005)—we would expect cells to be more excitable because β1 causes NaV channels to recover more quickly and increase channel availability, consistent with the Purkinje fiber role of fast conduction of cardiac excitation. During heart development, β1 expression increases, whereas β3 expression decreases (Domínguez et al., 2005; Okata et al., 2016). This dynamic temporal expression pattern suggests that β1 contributes more to mature NaV channel properties, such as increased excitability. Thus, we expect that molecular-level differences in β1 and β3 regulation of the DIII and DIV VSDs will affect organ-level behavior.
β1 and β3 localization within the NaV1.5 channel complex
The β1 subunit has been found to express and coassemble with both neuronal and cardiac NaV channels (Isom et al., 1992). There is a general consensus that when β1 coassembles with NaV1.5, it regulates SSI (Calhoun and Isom, 2014). However, the direction and magnitude of the inactivation shift vary depending on the expression system used and the protocols applied (Calhoun and Isom, 2014). This variability may be linked to the interaction between β1 and other members of the macromolecular NaV channel complex in native cells, such as ankyrin G (Malhotra et al., 2002). Thus, the nature of α–β1 interaction may vary by expression system, precluding the identification of a universal phenotype.
Several α–β1 interaction sites have been proposed. On the α subunit, a C-terminal mutation was able to eliminate β1 regulation of NaV1.1 current kinetics (Spampanato et al., 2004), and NaV1.4/NaV1.5 chimeras show that the S5–S6 linker of DIV plays a role in α–β1 interaction (Makita et al., 1996). Both results suggest that binding occurs near the DIV domain. Aside from the consequences of direct binding, the β1 subunit has also been shown to introduce the surface charges that electrostatically affect channel gating (Ferrera and Moran, 2006). We observed that WT β1 mainly affects the voltage dependence of DIV-VSD activation and its deactivation kinetics through possible direct interaction with the DIV-VSD, which suggests β1 proximity to the DIV-VSD. Together, these results support the hypothesis that β1 modulates inactivation by altering DIV-VSD activation. We infer that β1 most likely resides in the cleft between the DIII and DIV VSDs (Fig. 8, a and b).
The β3 subunit is homologous to β1 and also interacts with NaV channels noncovalently. Less is known about the α–β3 interaction. Because the β1 and β3 subunits are homologous (50%), it has been generally supposed that β3 interacts with the channel via the same mechanism as β1 (Namadurai et al., 2015). In contrast, we found that the WT β3 subunit caused a large depolarizing shift in DIII-VSD activation in addition to the DIV shift. The depolarization of DIII-VSD activation slowed down ionic current activation and inactivation kinetics, allowing the DIII-VSD to play a more prominent role in regulating this gating over a physiological range of potentials. Further, we demonstrated β3 proximity to the DIII S4 segment, as we observed that a tryptophan mutation on top of the β3 subunit strongly quenches the fluorophore attached to the DIII S3–S4 linker. It is most likely that β3 is adjacent to the DIII S4 segment in the cleft between the DII and DIII VSDs, which allows β3 to directly interact with the DIII-VSD. Altered DIII-VSD activation can then allosterically affect DIV-VSD activation. Yet, we observed that β3 still depolarized the DIV-VSD when the DIV VSD–pore coupling via the S4–S5 linker was abolished by the N1759A mutation, suggesting that this coupling to the DIV VSD takes place via alternative mechanisms, such as the DIII–DIV linker.
The importance of β3 in maintaining normal cardiac function has been highlighted by scn3b knockout mice. These mice exhibit slowed sinoatrial and atrioventricular conduction, burst pacing–induced atrial tachycardia, fibrillation, and ventricular tachycardia (Hakim et al., 2008, 2010). Consistent with our results (Fig. 2), knocking out β3 shifts NaV SSI to negative potentials, reducing peak Na+ current in the ventricle, which causes slowed conduction and decreased action potential duration in the endocardium and epicardium. Given the consistency of the knockout mouse phenotype with our results, we infer that β3 regulation of the NaV1.5 DIII and DIV VSDs significantly determines action potential morphology and conduction.
When we expressed β1 and β3 together, we observed enhancement of the depolarizing DIII-VSD shift and an exclusive effect of β1 on the DIV-VSD (Fig. 6). These results imply that the subunits do not interact with the channel independently. Previous work has shown that heterophilic interaction between the β1 and β3 subunits can occur via their respective Ig domains (Yereddi et al., 2013), which could possibly alter their interaction with each VSD. β3 binding to β1 may affect its interaction with the DIII-VSD. Our proposed localization of β1 and β3 would bring the extracellular domains of both subunits close to the DIII S4 (Fig. 8). In sum, our results show that both the β1 and β3 subunits are likely able to coassemble with a single NaV1.5 channel complex and that this coassembly significantly affects channel function.
Each domain of β1 and β3 has distinct interactions with the NaV1.5 channel
By measuring VSD conformation in the presence of β1/β3 chimeras, we showed that both the extracellular and transmembrane domains of β3 are necessary for β3 depolarization of the DIII-VSD. When channels were coexpressed with β1/β3 chimeras containing the β3 extracellular domain and the β1 transmembrane and intracellular domain, DIII-VSD activation was not depolarized, suggesting that the transmembrane domain of β3 is critical for localizing the β3 subunit to this location in NaV1.5, allowing the extracellular Ig domain of β3 to interact with the DIII-VSD. We also showed that the C terminus of the β1 subunit is necessary for relieving DIV-VSD immobilization and that the β3 C terminus is important for relieving DIII-VSD immobilization from fast inactivation (Fig. S5, c and e). Previously, we showed that the interaction between the fast inactivation gate (IFM) and the N1659 residue on the DIV S4–S5 linker plays an important role in immobilizing the DIII and DIV VSD. It is plausible that the β1 C terminus can interact with the intracellular DIV S5 segment, altering this interaction. These results show that β1 and β3 could interact with the NaV channel through multiple interaction sites and that both the extracellular domain and the C terminus play important roles in determining their gating properties.
Two-step movements of DIII VSD revealed by high expression of β3
As with KV channels, two-step transitions of NaV channel VSDs have been proposed based on previous observations that pulses of increasing duration alter the DIII-VSD deactivation rate (Varga et al., 2015; Hsu et al., 2017). Our results support this suggestion by showing that high levels of β3 subunit expression cause two prominent components of DIII VSD activation. This phenomenon is similar to KCNQ1 channels that show two-step activation in the presence of KCNE1, which stabilizes the activated VSD closed-pore state and slows ionic current activation (Barro-Soria et al., 2014). Similarly, the activation of Na+ current is slower when β3 separates the DIII-VSD transition into two components. Additionally, because NaV channels have very fast and prominent inactivation, the separation of DIII-VSD movement will increase the amplitude of the Na+ current as the channels are excited repetitively at a relatively high frequency (>3 Hz).
Our study shows that β1 and β3’s differential interactions with the DIII and DIV VSDs determine their regulation of Na+ current and cell excitability. These distinct regulatory mechanisms are essential for understanding how β subunits regulate excitable cells and how mutant β subunits cause disease.
We thank Dr. Stacey Rentschler, Dr. Henry Colecraft, James Ballard, Dr. Panpan Hou, and Dr. Roman Sloutsky for many helpful discussions and advice.
This study was funded by the e Fund Career Award from the Scientific Interface (grant 1010299); the National Institutes of Health (grant R01 HL136553 awarded to J.R. Silva); an American Heart Association fellowship (grant 15PRE25080073 awarded to W. Zhu); the Hungarian Academy of Sciences grant KTIA_NAP_13-2-2015-0009; a Bolyai fellowship (awarded to Z. Varga); and the National Heart, Lung, and Blood Institute of the National Institutes of Health (grant HL-034161 awarded to J.M. Nerbonne).
The authors declare no competing financial interests.
Richard W. Aldrich served as editor.