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Correction| April 30 2012

Molecular and biophysical basis of glutamate and trace metal modulation of voltage-gated Ca_v2.3 calcium channels

Aleksandr Shcheglovitov,

Aleksandr Shcheglovitov

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Iuliia Vitko,

Iuliia Vitko

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Roman M. Lazarenko,

Roman M. Lazarenko

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Peihan Orestes,

Peihan Orestes

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Slobodan M. Todorovic,

Slobodan M. Todorovic

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Edward Perez-Reyes

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Author and Article Information

Aleksandr Shcheglovitov , Iuliia Vitko , Roman M. Lazarenko , Peihan Orestes , Slobodan M. Todorovic , Edward Perez-Reyes

Online ISSN: 1540-7748

Print ISSN: 0022-1295

2012

J Gen Physiol (2012) 139 (5): 389.

https://doi.org/10.1085/jgp.2011106991395c

Volume 139, No. 3, March 5, 2012. Pages 219–234.

In the Results, in the second full paragraph on page 223 (“It was shown that Tricine…”), the sentence “This suggests that excitatory amino acids…” should not have included the word “excitatory.” The correct sentence is “This suggests that amino acids and Tricine could exert their effect on Ca_v2.3 by chelating trace amounts of Zn²⁺ and/or Cu²⁺, which commonly reside in normal bath solutions (Kay, 2004).”

In addition, the footnote b for Table 1 gave an incorrect p-value of P < 0.5. The correct p-value is P < 0.05. The table and corrected footnote are below.

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2012

TABLE 1

Biophysical properties

Solution	Activation				Inactivation kinetics
Ca_v2.3 WT	P_Ca(max) ± SEM	V_1/2 ± SEM	k ± SEM	n	V_1/2 ± SEM	k ± SEM	n	A_f ± SEM	τ_f ± SEM	A_s ± SEM	τ_s ± SEM	n
HEPES	0.011 ± 0.003	1.5 ± 1.0^a	7.2 ± 0.1^a	8	−36.1 ± 1.1^b	8.9 ± 0.2	8	−0.24 ± 0.05^b	0.17 ± 0.02	−0.59 ± 0.04	1.5 ± 0.2	6
Tricine	0.010 ± 0.002	−15.6 ± 1.8	5.3 ± 0.2	12	−40.2 ± 1.2	8.2 ± 0.3	11	−0.38 ± 0.03	0.13 ± 0.01	−0.44 ± 0.02	1.2 ± 0.1	6
Zn 7 μM	0.005 ± 0.001	−2.4 ± 2.9^a	7.1± 0.1^a	4	−30.4 ± 1.8^a	9.8 ± 0.5	6	−0.14 ± 0.01^a	0.33 ± 0.08	−0.52 ± 0.06	2.4 ± 0.6	6
Cu 205 nM	0.007 ± 0.002	2.9 ± 1.2^a	7.4± 0.1^a	6	−26.8 ± 1.2^a	9.7 ± 0.8	6	−0.18 ± 0.04^a	0.21 ± 0.08	−0.48 ± 0.04	3.1 ± 0.4^b	6
H179E/H181A
HEPES	0.010 ± 0.002	−12.7 ± 1.0	5.0 ± 0.2	7	−42.9 ± 1.8	9.1 ± 0.4	6	−0.32 ± 0.04	0.14 ± 0.02	−0.59 ± 0.04	1.3 ± 0.1	5
Tricine	0.010 ± 0.001	−12.1 ± 1.1	5.2 ± 0.2	13	−41.2 ± 1.3	8.4 ± 0.2	8	−0.34 ± 0.03	0.14 ± 0.01	−0.55 ± 0.02	1.2 ± 0.1	5
Zn 7 μM	0.008 ± 0.002	−9.4 ± 0.9	6.0 ± 0.3	5	−36.0 ± 2.4	9.2 ± 0.8	4	−0.31 ± 0.04	0.23 ± 0.11	−0.65 ± 0.17	2.8 ± 1.1	3
Cu 205 nM	0.009 ± 0.002	−8.6 ± 1.9	5.6 ± 0.2	8	−37.2 ± 1.1	8.0 ± 0.4	6	−0.33 ± 0.02	0.20 ± 0.06	−0.64 ± 0.12	2.4 ± 0.7	5

Solution	Activation				Inactivation kinetics
Ca_v2.3 WT	P_Ca(max) ± SEM	V_1/2 ± SEM	k ± SEM	n	V_1/2 ± SEM	k ± SEM	n	A_f ± SEM	τ_f ± SEM	A_s ± SEM	τ_s ± SEM	n
HEPES	0.011 ± 0.003	1.5 ± 1.0^a	7.2 ± 0.1^a	8	−36.1 ± 1.1^b	8.9 ± 0.2	8	−0.24 ± 0.05^b	0.17 ± 0.02	−0.59 ± 0.04	1.5 ± 0.2	6
Tricine	0.010 ± 0.002	−15.6 ± 1.8	5.3 ± 0.2	12	−40.2 ± 1.2	8.2 ± 0.3	11	−0.38 ± 0.03	0.13 ± 0.01	−0.44 ± 0.02	1.2 ± 0.1	6
Zn 7 μM	0.005 ± 0.001	−2.4 ± 2.9^a	7.1± 0.1^a	4	−30.4 ± 1.8^a	9.8 ± 0.5	6	−0.14 ± 0.01^a	0.33 ± 0.08	−0.52 ± 0.06	2.4 ± 0.6	6
Cu 205 nM	0.007 ± 0.002	2.9 ± 1.2^a	7.4± 0.1^a	6	−26.8 ± 1.2^a	9.7 ± 0.8	6	−0.18 ± 0.04^a	0.21 ± 0.08	−0.48 ± 0.04	3.1 ± 0.4^b	6
H179E/H181A
HEPES	0.010 ± 0.002	−12.7 ± 1.0	5.0 ± 0.2	7	−42.9 ± 1.8	9.1 ± 0.4	6	−0.32 ± 0.04	0.14 ± 0.02	−0.59 ± 0.04	1.3 ± 0.1	5
Tricine	0.010 ± 0.001	−12.1 ± 1.1	5.2 ± 0.2	13	−41.2 ± 1.3	8.4 ± 0.2	8	−0.34 ± 0.03	0.14 ± 0.01	−0.55 ± 0.02	1.2 ± 0.1	5
Zn 7 μM	0.008 ± 0.002	−9.4 ± 0.9	6.0 ± 0.3	5	−36.0 ± 2.4	9.2 ± 0.8	4	−0.31 ± 0.04	0.23 ± 0.11	−0.65 ± 0.17	2.8 ± 1.1	3
Cu 205 nM	0.009 ± 0.002	−8.6 ± 1.9	5.6 ± 0.2	8	−37.2 ± 1.1	8.0 ± 0.4	6	−0.33 ± 0.02	0.20 ± 0.06	−0.64 ± 0.12	2.4 ± 0.7	5

All parameters were calculated for each cell and then averaged. Statistically significant differences are noted with footnotes and are by one-way ANOVA, compared to the values obtained in Tricine:

P < 0.001.

P < 0.05.

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Molecular and biophysical basis of glutamate and trace metal modulation of voltage-gated Ca_v2.3 calcium channels