Many studies of Ca2+ signaling use PC12 cells, yet the balance of Ca2+ clearance mechanisms in these cells is unknown. We used pharmacological inhibition of Ca2+ transporters to characterize Ca2+ clearance after depolarizations in both undifferentiated and nerve growth factor-differentiated PC12 cells. Sarco-endoplasmic reticulum Ca2+ ATPase (SERCA), plasma membrane Ca2+ ATPase (PMCA), and Na+/Ca2+ exchanger (NCX) account for almost all Ca2+ clearance in both cell states, with NCX and PMCA making the greatest contributions. Any contribution of mitochondrial uniporters is small. The ATP pool in differentiated cells was much more labile than that of undifferentiated cells in the presence of agents that dissipated mitochondrial proton gradients. Differentiated PC12 cells have a small component of Ca2+ clearance possessing pharmacological characteristics consistent with secretory pathway Ca2+ ATPase (SPCA), potentially residing on Golgi and/or secretory granules. Undifferentiated and differentiated cells are similar in overall Ca2+ transport and in the small transport due to SERCA, but they differ in the fraction of transport by PMCA and NCX. Transport in neurites of differentiated PC12 cells was qualitatively similar to that in the somata, except that the ER stores in neurites sometimes released Ca2+ instead of clearing it after depolarization. We formulated a mathematical model to simulate the observed Ca2+ clearance and to describe the differences between these undifferentiated and NGF-differentiated states quantitatively. The model required a value for the endogenous Ca2+ binding ratio of PC12 cell cytoplasm, which we measured to be 268 ± 85. Our results indicate that Ca2+ transport in undifferentiated PC12 cells is quite unlike transport in adrenal chromaffin cells, for which they often are considered models. Transport in both cell states more closely resembles that of sympathetic neurons, for which differentiated PC12 cells often are considered models. Comparison with other cell types shows that different cells emphasize different Ca2+ transport mechanisms.

The PC12 clonal cell line is derived from a cancer of rat adrenal chromaffin cells, a pheochromocytoma (Greene and Tischler, 1976). Rapidly dividing PC12 cells are regarded as chromaffin cell–like, having dense-core secretory granules and the ability to release catecholamines upon stimulation. The cells undergo a marked phenotypic change in response to nerve growth factor (NGF) and certain other substances: they stop dividing, extend numerous neural processes (neurites), and up-regulate several neural markers (Greene and Tischler, 1976; Vaudry et al., 2002). Thus, PC12 cells also could be considered models of sympathetic neurons and of neural differentiation.

Almost 2,000 publications concern calcium effects in this cell line. Much is therefore known about protein expression, including Ca2+ channels and transporters, yet in neither undifferentiated nor differentiated PC12 cells is much known about the Ca2+ transport itself. This is surprising, as these excitable cells are so often used as models for chromaffin cells or sympathetic neurons. We have characterized Ca2+ transport in PC12 cells in order to answer the following questions. What are the principal pathways for Ca2+ clearance? Do they change in response to NGF? Does this Ca2+ clearance resemble clearance in chromaffin cells or in sympathetic neurons? We used Ca2+ photometry and electrophysiology to characterize Ca2+ buffering and transport in both undifferentiated and NGF-differentiated PC12 cells, and we focus on the four canonical transport mechanisms commonly found in mammalian cells: sarco/endoplasmic reticulum Ca2+ ATPases (SERCA), mitochondrial uniporter(s) (MtU), plasma membrane Ca2+ ATPases (PMCA), and Na+/Ca2+ exchangers (NCX).

All chemicals were obtained from Sigma-Aldrich unless otherwise noted.

Culture and Differentiation of PC12 Cells

PC12 D19 cells were a gift from Sandra Bajjalieh in the Department of Pharmacology, University of Washington (the lineage of these cells is traced in the online supplemental material). Cells were cultured in Dulbecco's Modified Eagle Medium with high glucose, L-glutamine, pyridoxine hydrochloride, and sodium pyruvate (Invitrogen), supplemented with 10% horse serum (Gemini Bioproducts), 5% FBS (Gemini Bioproducts), 44 mM sodium bicarbonate, and 25 mM HEPES, pH to 7.3 with NaOH. Cells were maintained in a 5% CO2 atmosphere and passaged 1:20 with Ca2+- and Mg2+-free PBS (Invitrogen) weekly. Cells were subcultured onto polyornithine-coated number 0 glass coverslips for experiments.

To differentiate cells, we subcultured them onto the coated coverslips at a low density and cultured them in the normal culture medium for 2 h. We then replaced this medium with medium supplemented with 50 ng/ml NGF (gift of M. Bothwell, University of Washington). NGF medium was renewed every third day for a minimum of 15 d. Neurite extension was evident as early as day 3, but we waited longer in order to reach a stage of differentiation in which all neural markers would be up-regulated.

Ca2+ Photometry

Cytoplasmic Ca2+ measurements were done at elevated temperature by ratiometric fura-2 or fura-4F (Invitrogen) photometry. Cells were loaded with the acetoxymethyl (AM) ester of the desired dye dispersed in 10% pluronic and diluted to 10 μM in modified Ringer's solution (in mM: 130 NaCl, 2.5 KCl, 1 MgCl2, 2 CaCl2, 10 glucose, and 10 HEPES, pH 7.3) at room temperature for 20–25 min and transferred into a recording chamber perfused with the modified Ringer's solution using a local perfusion device heated to 37°C. To determine the [fura-2] in PC12 cells, we loaded cells with known concentrations of fura-2 salt using whole-cell patch configuration (see below). By comparing the amount of light collected from resting cells so loaded to similarly sized cells loaded with fura-2-AM, we estimated that the average PC12 cell contains 300 nM fura-2 under our loading conditions. This number was used in our mathematical model and in translating the rate of change of [Ca2+]cyt into absolute fluxes. We used a 3-s or, in Fig. 1 C only, 30-s exposure to high-K+ depolarizing solution to impose a cytoplasmic Ca2+ load. This solution contained in mM: 70 KCl, 67 NaCl, 2 CaCl2, 1 MgCl2, 10 glucose, and 10 HEPES, pH 7.3. In control experiments, a nominally Ca2+-free solution (modified Ringer's without CaCl2) was applied to the cells as they recovered from depolarization. The composition of this clearance solution and of the high K+ solution was sometimes changed as noted. Nominally Ca2+-free Ringer's solution was adequate to prevent store-operated currents activated by thapsigargin from contaminating the measurements (Fig. S1). We recorded only from one cell on each coverslip to ensure that each recorded cell was “naïve” with respect to previous Ca2+ elevations.

Fura fluorescence was excited by 10 ms of 340-nm illumination followed immediately by 10 ms of 380-nm illumination (TILL Photonics), and emission at 505 nm was collected by a photodiode controlled by PatchMaster software (HEKA Electronics). The first 2 ms of each collection was discarded to allow time for the optical transitions of the illuminator. [Ca2+]cyt was calculated from the background-corrected fluorescence ratio R = F340/F380 using the standard calibration equation: [Ca2+]cyt = K*[(R − Rmin)/(Rmax − R)], where R is the measured ratio, and the calibration parameters K*, Rmin, and Rmax were experimentally determined as previously reported (Chen et al., 2003). Fura-2 and fura-4F required separate calibrations. For Ca2+ transport measurements, which used fura-2, all points >1500 nM Ca2+ were discarded as unreliable.

The setup included a viewfinder that allowed us to exclude some regions of the field of view from the measurements. We took advantage of this to restrict measurements to the soma or to a field of neurites (in NGF-differentiated cells). Due to the larger volume of the somata, fura fluorescence collected from them was substantially larger than fluorescence collected from neurites. To prevent contamination of signals collected from neurites with nearby somata, we recorded from neurites only when we could exclude all somata completely from the field of view. It is likely that the neurites from several cells were in the same field of measurement.

Inhibition of Ca2+ Transporters

SERCA were inhibited by adding 1 μM thapsigargin (TG) to the fura-loading medium. After TG treatment, [Ca2+]cyt became responsive to extracellular Ca2+, demonstrating activation of store-operated currents and indicating successful SERCA inhibition (Ufret-Vincenty et al., 1995). The MtU was inhibited by applying 2 μM CCCP to the cells both during the KCl depolarization and throughout Ca2+ clearance (Herrington et al., 1996). In later experiments, 5 μM oligomycin (EMD BioSciences) was added either concomitantly with CCCP or 30 s before depolarization and maintained through the rest of the experiment. For the inhibition of MtU in neurites, cells were pretreated with 10 μM Ru360 (EMD BioSciences) for 30 min before Ca2+ measurement (Thomas et al., 2006). For the inhibition of plasma membrane Ca2+ ATPases (PMCA), extracellular pH was raised to 9.0 during clearance by replacing the HEPES buffer in the nominally Ca2+-free modified Ringer's with 20 mM Trizma, pH 9.0 (Xu et al., 2000). NCX were inhibited by replacing the NaCl in the nominally Ca2+-free modified Ringer's with LiCl. Any experiment in which only one transporter is blocked is referred to as a “1-blocked” experiment. Our results are generally displayed as Ca2+ transport curves by plotting the rate of change of free Ca2+ (−d[Ca2+]cyt/dt) as a function of [Ca2+]cyt. To obtain the Ca2+ transport curve for the inhibited mechanism in a 1-blocked experiment, we subtracted the 1-blocked Ca2+ transport curve from the control Ca2+ transport curve. In many experiments, we combined two of these treatments (2-blocked), three of these treatments (3-blocked), or all four (4-blocked). When combining treatments, TG was always used to inhibit SERCA, CCCP to inhibit MtU (unless otherwise noted), pH 9.0 to inhibit PMCA, and LiCl to inhibit NCX. In some experiments, we added a fifth inhibitor to the 4-blocked protocol, 10 μM tert-butylhydroquinone (BHQ), to inhibit secretory pathway Ca2+ ATPases (SPCA), as well as SERCA. The Ca2+ peak heights and rise times were unaffected by these inhibitors of Ca2+ transport. Also, some cells were pretreated with 50 μM glycyl phenylalanyl β-napthylamide (GPN) for 20 min before Ca2+ measurements (Duman et al., 2006).

Determination of the Ca2+ Binding Ratio of Cytoplasm (κi)

For every free cytoplasmic Ca2+ ion, κi ions are bound reversibly to intracellular buffers. κi was determined using simultaneous fura-2 photometry and standard whole-cell recording of inward Ca2+ currents (ICa). The intracellular solution contained (in mM): 125 cesium glutamate, 5 NaCl, 2 Mg⋅ATP, 0.3 Na2GTP, 1 MgCl2, 0.2 fura-2, and 20 HEPES, pH 7.2 with CsOH. The extracellular solution contained (in mM): 140 NaCl, 2.8 KCl, 10 d-glucose, 1.2 MgCl2, 2 CaCl2, and 10 HEPES, pH 7.4 with NaOH. PC12 cells were clamped at −80 mV and depolarized to 0 mV for 1 s to elicit Ca2+ influx through voltage-gated Ca2+ channels. Whole-cell currents (with P/4 subtraction) and fura fluorescence (as above) were recorded, and κi was calculated using the equation:

\[{\kappa}_{\mathrm{i}}{=}{\int}({-}\mathrm{I}_{\mathrm{Ca}}(\mathrm{t})\mathrm{dt})/\mathrm{zFv}{\cdot}{\Delta}[\mathrm{Ca}^{2{+}}],\]
(1)

where ICa(t) is empirically measured current using the patch clamp, z is the valence of Ca2+, F is Faraday's constant, v is cellular volume calculated from the capacitance of the cell assuming a sphere, and Δ[Ca2+] is the peak increase determined from the fura-2 measurements. κi represents the contributions of all buffers in the cell, including fura-2. To extract the contribution of endogenous buffers, we split κi into κi endo and the known fura-2 component:

\begin{eqnarray*}&&{\kappa}_{\mathrm{i}}{=}{\kappa}_{\mathrm{i{\,}endo}}{+}\\&&[\mathrm{fura}-2]{\ast}\mathrm{K}_{\mathrm{D,{\,}fura}-2}/(\mathrm{K}_{\mathrm{D,{\,}fura}-2}{+}[\mathrm{Ca}^{2{+}}]_{1\mathrm{cyt}})(\mathrm{K}_{\mathrm{D,{\,}fura}-2}{+}[\mathrm{Ca}^{2{+}}]_{2\mathrm{cyt}}),\end{eqnarray*}
(2)

where κi, endo is the endogenous Ca2+ binding ratio of the cytoplasm, and [Ca2+]1cyt and [Ca2+]2cyt represent [Ca2+]cyt recorded before and after membrane depolarization (Zhou and Neher, 1993). The concentration of fura-2 in the intracellular pipette solution is 200 μM and its dissociation constant for Ca2+ (KD) was taken as 224 μM.

Data Analysis

Fluorescence intensities were recorded using PatchMaster, corrected for background, and converted into ratios and then free Ca2+ using IGOR Pro (Wavemetrics). For each individual Ca2+ trace, we considered only the clearance phase that follows Ca2+ loading and used IGOR to calculate the derivative d[Ca2+]cyt/dt for each data point during Ca2+ clearance. The derivative was calculated as the slope of the line between the point immediately before the desired point and the point immediately after; points were 0.5 s apart. The first two to three points on all traces were discarded. During net clearance, d[Ca2+]cyt/dt has a negative value by convention. To simplify the presentation, we plot −d[Ca2+]cyt/dt, so that as the rate of Ca2+ transport out of the cytoplasm falls, the curve falls. Fig. 1 A shows typical time courses of Ca2+ in cells that underwent KCl depolarization and clearance, and Fig. 1 B shows the derivatives obtained from these single cells.

The error bars and statistics given for means represent SEM. To combine data from multiple cells, we binned data points by the values of [Ca2+]cyt. The bins were 100 nM wide from 0 to 1000 nM, and 200 nM wide above 1000 nM. We then averaged the binned data from a pool of cells—[Ca2+]cyt for the abcissae and d[Ca2+]cyt/dt for the ordinates—and report the average value in the figures. Note that due to a large spread in peak Ca2+ values (Fig. 5), not every cell that we measured contributed to every bin. All of these mean Ca2+ transport curves (d[Ca2+]cyt/dt vs. [Ca2+]cyt) are shown ± SEM of both x and y values. At times, we performed mathematical operations on Ca2+ transport curves. Most frequently, this involved addition and subtraction operations. To add or subtract curves, we averaged the concentrations and performed the desired operation on the transport rates for each bin. SEMs combined according to the standard formula for addition (Goldstein, 1964).

Throughout the manuscript, curves that were obtained directly from experimental data are shown with open symbols and solid lines; the lines may connect the symbols or may represent output of a model. Points from mathematical operations performed on experimental results are shown with closed symbols and dotted lines. With the exception of the control Ca2+ transport curves, curves that have appeared in previous figures are shown as lines and error bars without symbols. In Fig. 9, we bypassed some of our rules and switched to closed symbols in panels A, B, C, and D for clarity.

Online Supplemental Material

Figs. S1 and S2 show the responsiveness of baseline [Ca2+]cyt to extracellular Ca2+ after TG treatment and theoretical calculations of local depletion of Ca2+ near the plasma membrane when Ca2+ transporters are at work. Additional supplementary text describes the provenance of the PC12 cells we used and lists the computer program used in the calculation of local Ca2+ depletion.

Preliminary Observation of Ca2+ Clearance

PC12 cells were depolarized with 3-s pulses of 70 mM KCl to induce Ca2+ entry, and the cytoplasmic Ca2+ ([Ca2+]cyt) was monitored using fura-2 preloaded into cells in its acetoxymethyl ester form. Fig. 1 A shows the Ca2+ time course for an experiment on a single cell that exhibited average rates of Ca2+ clearance (black line, control). During KCl treatment, [Ca2+]cyt increased rapidly, and then upon removal of KCl it fell back more slowly to the baseline, representing Ca2+ clearance. In this figure and elsewhere we have also drawn smooth curves (red, simulated control) derived from a mathematical model of the transport mechanisms described in Appendix. The black circles in Fig. 1 B (control) show the time derivative of [Ca2+]cyt during the clearance phase plotted versus [Ca2+]cyt as a Ca2+ transport curve for the same cell. As expected, the net clearance rate −d[Ca2+]cyt/dt fell from its peak value (top right) as [Ca2+]cyt fell. Results from many cells were pooled and binned by the [Ca2+]cyt values in the abscissa to obtain the black curve in Fig. 1 C. This method of analysis allowed us to pool results from many cells despite differences in peak cytoplasmic Ca2+.

Before resolving the mechanistic components of this clearance, we need to consider whether Ca2+ transport rates depend only on instantaneous [Ca2+]cyt or are also influenced by the past history of Ca2+ exposure. To look for history dependence, we lengthened the KCl pulse to 30 s (Fig. 1, D–F, blue line). As was typical of several repeats of this protocol, Ca2+ continued to increase well above the useful level for fura-2 for 8–10 s during the pulse and then “sagged” (Fig. 1 D). Fig. 1 D compares 30-s and 3-s depolarizations. After the 30-s depolarization, clearance was slower and Ca2+cyt remained above baseline, even 70 s later. Transport curves for these two cells are compared in Fig. 1 E, and mean values for pooled data from many cells are shown in Fig. 1 F. Clearance rates after 30 s are less that half those after 3 s. A possible explanation for the slowing is that the Ca2+ level in intracellular stores rises during prolonged Ca2+ elevation, altering their ability to continue to clear Ca2+ or even causing Ca2+ to be released from the stores, further blunting apparent Ca2+ clearance. We tested whether this might involve the ER by pretreating the cells with thapsigargin (TG) to inhibit SERCA and to empty ER Ca2+ stores; however, TG did not change the Ca2+ clearance seen after a 30-s depolarization (Fig. 1 F). These experiments indicate that (a) Ca2+ clearance in PC12 cells is slower after long depolarizations, (b) SERCA pumps do not serve to clear Ca2+ after 30-s depolarization (in contrast to 3-s depolarization, see below), and (c) release of Ca2+ from the ER during clearance does not contribute to the apparent slowing of Ca2+ clearance after long depolarizations. The unexplained slowing could be due to release of Ca from other stores that fill during the extended depolarization or to modifications (phosphorylations?) of transporters induced by the massive, unphysiological Ca2+ elevation. We chose to avoid these complications in the rest of this paper by focusing only on recovery after 3-s depolarizations and by using only “naive” cells that had never been exposed to previous KCl pulses.

Ca2+ Clearance in Undifferentiated PC12 Cells

We dissected the contributions of various Ca2+ transporters to total Ca2+ clearance after 3-s depolarizations using the conditions described in Materials and methods to inhibit each mechanism. SERCA was inhibited by pretreatment with 1 μM thapsigargin (TG), MtU was inhibited by acute application of 2 μM CCCP, PMCA was inhibited by acutely lowering extracellular proton concentration (pH raised to 9.0), and NCX was inhibited by replacing extracellular Na+ with Li+. We started by blocking all four mechanisms simultaneously to determine whether they accounted for the observed Ca2+ transport. The results of these 4-blocked experiments are shown in Fig. 1 as gray symbols. Fig. 1 A shows a sample time course of intracellular Ca2+, and Fig. 1 B shows the derivatives obtained from that cell. Although Ca2+ transport is much slower in the 4-blocked cell, [Ca2+]cyt does eventually return to baseline in this cell. Of the 62 cells treated with the 4-blocked protocol, 43 (∼70%) returned to baseline within 90 s, albeit with greatly slowed kinetics. The remaining cells arrested before reaching the baseline, at an average [Ca2+]cyt of ∼650 nM. This arrest is not due to Ca2+ entering through a store-operated pathway activated by TG because the clearance phase was performed in nominally Ca2+-free Ringer's, and we observed that TG-treated cells maintained under this condition did not have elevated basal [Ca2+]cyt. The pooled 4-blocked transport curve from the 62 experiments is compared with that for 88 controls in Fig. 1 C. Blocking the four transporters inhibits over 90% of the Ca2+ transport above 300 nM [Ca2+]cyt. Thus most Ca2+ transport in PC12 cells is accounted for by the four classical mechanisms. We refer to the small remaining transport as “residual Ca2+ transport.” It could represent additional minor Ca2+ clearance mechanisms, possible slow binding to some cytoplasmic buffers, and imperfect block of the classical transport mechanisms. For example, pH 9.0 should block only 93% of the PMCA activity, leaving a residual of 7% (Xu et al., 2000).

We then assessed the capacity of each of these classes of transporters by performing 3-blocked experiments in which we blocked three classes of transporters and examined the ability of the unblocked transporter to clear Ca2+. The results of these experiments are shown in Fig. 2. In each panel, the control curve represents transport in the absence of inhibitors, and the remaining curves in Fig. 2 (A and B) show the transport when only one of the classical mechanisms is operating alone; these curves have been corrected for the small residual Ca2+ transport that remains in the 4-blocked experiments (Fig. 1 C). We call the results of these experiments “capacity curves” because they show what one transport mechanism could accomplish essentially alone. Fig. 2 A shows the capacity of the two intracellular Ca2+ transporters. Compared with overall transport, the SERCA makes little contribution except above 800 nM [Ca2+]cyt. The MtU component is practically negligible, being comparable to the residual transport component of 4-blocked experiments (Fig. 1). Fig. 2 B shows the capacities of the two plasma-membrane transporters. The PMCA and NCX each account for almost half of the total Ca2+ transport up to 900 nM [Ca2+]cyt. In accordance with work on other cells, at higher concentrations, the PMCA appears to saturate, whereas the NCX rate continues to rise and accounts for ∼70% of the total Ca2+ transport. Fig. 2 C shows the sum of the capacity curves for these four transporters plus the residual Ca2+ transport compared with the observed transport in the control. Together, these data show that the Ca2+ transporting capacity of PC12 cells is dominated by the two plasma membrane transporters and that, up to 1000 nM [Ca2+], the transporters are operating near capacity.

Are Clearance Mechanisms Independent?

If the four clearance mechanisms operated independently, any combination of them should be additive. To verify additivity and to cross-check the conclusions of our 3-blocked measurements, we performed a series of 1-blocked experiments in which only one transporter was inhibited at a time. The “activity” of that transporter was determined by subtracting the 1-blocked curve from the control curve. We refer to the subtracted curves as “activity curves” (Fig. 3). An activity curve differs from a capacity curve in that the former is intended to show what a given transporter accomplishes while working in concert with other Ca2+ transporters, whereas the latter shows the results of that transporter working alone. The panels on the left show the 1-blocked transport curves in gray and the control curves in black. The panels on the right show the activity curves for each mechanism, i.e., the differences between the two curves in the left panel, in gray, compared with the capacity curves obtained from the 3-blocked experiments (Fig. 2) in black. The activity curves are quite noisy as each point represents a small difference between two large numbers. For SERCA pumps, the capacity and activity curves are not obviously different, transport remaining small until [Ca2+]cyt rises above 1000 nM (Fig. 3, A and B). Likewise, for the MtU, the curves seem similar and small at all but high (≥800 nM) values of [Ca2+]cyt (Fig. 3, C and D). For PMCA, on the other hand, the calculated activity falls well below the capacity for five consecutive data points below 800 nM [Ca2+]cyt (Fig. 3, E and F). Under these 1-blocked conditions, the NCX was the most robust transporter of Ca2+, and it operated at capacity until ∼1000 nM Ca2+; where it fell below the capacity curve (Fig. 3, G and H). These calculations indicate that the canonical Ca2+ transporters transport at or a little below capacity over the tested range of [Ca2+]cyt, explaining why the sum of individual transport capacities slightly exceeds the observed total transport at higher values of [Ca2+]cyt (Fig. 2 C). The deviation from additivity for the NCX, for example, might imply that when the other transporters are operating, the NCX experiences a lower local [Ca2+]cyt than the average value that fura-2 dye reports (see Discussion).

In addition to the four canonical mechanisms of Ca2+ transport, SPCAs can transport Ca2+ into the Golgi and perhaps into distal secretory compartments. SPCAs are not inhibited by TG but are reported to be inhibited by high concentrations of BHQ (Wuytack et al., 2002). We performed a 5-blocked experiment in which we blocked SPCA with added 10 μM BHQ after blocking the other four mechanisms of Ca2+ transport with the 4-block cocktail. Surprisingly, adding BHQ slightly speeded Ca2+ clearance above 800 nM [Ca2+]cyt (Fig. 4 A). This might occur if SPCAs normally fill some compartment with Ca2+ that releases the Ca2+ when [Ca2+]cyt is high; BHQ would prevent this compartment from being full, so that less Ca2+ is released into the cytoplasm during the clearance phase in the presence of BHQ. The effect is small. In a previous study, we observed that dense core secretory granules release Ca2+ after plasma membrane depolarization in pancreatic β cells and were disrupted by the dipeptide GPN (Duman et al., 2006). Therefore we wanted to see whether the BHQ-sensitive compartment in PC12 cells also was disturbed by pretreatment for 20 min with 50 μM GPN. Below 800 nM [Ca2+]cyt, clearance was completely unaltered by GPN (Fig. 4 B). Unfortunately, we were unable to collect data from high Ca2+ ranges because these cells exhibited lower peak Ca2+ values than control cells did. The experiment was therefore not conclusive.

Ca2+ Clearance from Somata of Differentiated PC12 Cells

We next asked whether cellular Ca2+ clearance mechanisms are changed when PC12 cells are differentiated by >15 d culture in 50 ng/ml NGF (see Materials and methods). With undifferentiated cells, the KCl-induced peak of [Ca2+]cyt sometimes rose above the reliable range of the fura-2 indicator; and with differentiated cells this happened more often. Therefore we turned to the lower-affinity Ca2+ dye fura-4F for more reliable measurements of the height of [Ca2+]cyt peaks. Both differentiated and undifferentiated cells showed a wide range of peak heights in response to KCl depolarization (Fig. 5). The average, maximal, and range of peak heights were all markedly higher in NGF-differentiated cells. Higher [Ca2+]cyt peaks might be expected from the greater surface-to-volume ratio of differentiated cells and from a reported increase in Ca2+ current (Black et al., 2003).

Fig. 6 A compares the Ca2+ transport curves for somata of NGF-differentiated (black line) and undifferentiated cells (gray line). They are very similar, with that for undifferentiated cells lying slightly above that for differentiated cells. When the 4-blocked protocol was applied to differentiated cells, we found that it abolished virtually all transport when [Ca2+]cyt was <1000 nM but left some residual Ca2+ transport above this value (Fig. 6 B). Adding 10 μM BHQ on top of the 4-blocked protocol (i.e., a 5-blocked experiment) decreased residual Ca2+ transport by ∼50% (Fig. 6 C). Thus, differentiation introduces a measurable component of Ca2+ clearance flux (out of the cytoplasm) that is not eliminated by TG but is BHQ sensitive. This flux could represent SPCA transporters on non-ER organelles of the secretory pathway. Unlike undifferentiated cells, the differentiated cells showed no depression of maximal Ca2+ rise after GPN treatment. GPN pretreatment had no effect on Ca2+ transport (Fig. 6 D), suggesting that the putative SPCA-containing compartment is not lysosomal.

We determined the capacity of the four canonical transporters in differentiated cells by performing 3-blocked experiments, and we determined their activities when other transporters were active using 1-blocked experiments. As shown in Fig. 7, the capacity curves nearly overlaid the noisier activity curves for SERCA, PMCA, and NCX in differentiated cells. However, the component of transport attributed to MtU is clearly discrepant between the two measures. Application of CCCP at the moment of the KCl treatment diminished subsequent Ca2+ transport by almost 50% at [Ca2+]cyt values above 1000 nM (Fig. 7 C). At face value, this result contrasted with our 3-blocked experiments, which suggested that the mitochondria might even release a little Ca2+ into the cytoplasm at these high values of [Ca2+]cyt and transported little net Ca2+ below 1000 nM [Ca2+]cyt (Fig. 7 D). Clearly in differentiated cells the 1-blocked (CCCP) experiments are not correctly extracting the mitochondrial component of flux seen with the three blocked experiments (high pH, TG, Li+).

Why does CCCP block so much clearance activity in the 1-blocked experiments? We suggest that even a short CCCP treatment stops not only the MtU but also the two ATP-requiring transporters. CCCP collapses the mitochondrial proton gradient, stopping formation of new ATP by mitochondria and allowing cellular ATP to deplete if glycolysis cannot keep up with ATP demand. Treatment with oligomycin can be used to keep the F1F0 ATPase from breaking down ATP once the proton gradient is gone (Babcock et al., 1997). However, addition of 5 μM oligomycin, either coincident with CCCP or as a 30-s preincubation before CCCP was added, only weakly restored some missing Ca2+ transport (Fig. 8 A). Oligomycin had little effect on residual Ca2+ transport in 4-blocked experiments (Fig. 8 B). To determine which non-MtU transporters were affected by CCCP, we performed a series of 2-blocked experiments, treating the cells with both CCCP and one other inhibitor and allowing the other two mechanisms to function. We compared these results to curves calculated from the 3-blocked experiments in Fig. 7. For example, in Fig. 8 C, we compare a 2-blocked experiment in which MtU and SERCA are inhibited (black line) with the calculated sum of the capacities of NCX, PMCA, and the residual Ca2+ transport (gray line). The two curves are similar, suggesting that the fluxes allowed to operate in this experiment are not strongly affected by CCCP. In Fig. 8 D, we inhibited both MtU and PMCA (black line) and compared the transport curve to the sum of the capacities of SERCA, NCX, and the residual Ca2+ transport. Once again, the curves are quite similar with CCCP being only slightly lower. However, when we inhibited NCX in addition to CCCP treatment, we obtained the unambiguous result that the remaining transport was far below that expected for the sum of SERCA, PMCA, and the residual Ca2+ transport (Fig. 8 E). In fact, the observed transport was virtually identical to residual Ca2+ transport. These results indicate that both SERCA and PMCA are inhibited by CCCP treatment, as might be expected since both require ATP, and that when only one of these two transporters is working, a differentiated cell can supply almost enough ATP to keep up, but when both are operating it cannot.

The results in Fig. 8 cast suspicion on the capacity measurements made in Fig. 7, since some were based on 3-blocked experiments that contained CCCP. Therefore, we redetermined capacity for canonical transporters in differentiated cells using another series of 2-blocked experiments. (The capacity measurement for MtU was unaffected, however, because this was the one 3-blocked experiment that did not contain CCCP.) For determination of the capacity of each transporter, we inhibited the other two non-MtU transporters. We then corrected the resulting transport curve for the contributions of both MtU and residual Ca2+ transport. We compared these fluxes with capacity curves obtained from 3-blocked experiments performed on undifferentiated cells (Fig. 2). Strikingly, differentiation left many aspects of Ca2+ clearance unchanged. Not only are the overall control transport curves for undifferentiated and NGF-differentiated cells scarcely distinguishable from each other (Fig. 6 A), the capacity curves for SERCA are essentially the same in both undifferentiated and NGF-differentiated cells (Fig. 9 A), and they are small. The mitochondria remain a negligible component showing no net uptake in differentiated cells (Fig. 9 B). However, the capacities of both PMCA and NCX change appreciably and reciprocally with differentiation. PMCA capacity increases and NCX capacity decreases (Fig. 9, C and D). Finally, residual Ca2+ transport does not change appreciably after NGF differentiation (Fig. 9 E). As was the case for the undifferentiated cells, the sum of the four capacity traces and the residual Ca2+ transport trace for differentiated cells gives a reasonable estimate for observed total cellular Ca2+ transport (Fig. 9 F), although with some indication that transporters are working below capacity above 800 nM [Ca2+]cyt.

To describe the changes between differentiated and undifferentiated cells quantitatively, we fitted the capacity curves with mathematical functions that describe Ca2+ transport due to each mechanism (see Appendix for details). The smooth lines in Fig. 2, Fig. 4 A, and Fig. 9 show these functions using the parameters given in Table I. A small increase in apparent cooperativity (reflected in the Hill coefficient, nHill) was the most marked change in SERCA caused by differentiation. PMCA underwent an almost twofold increase in maximal velocity (Mmax), a decrease in Ca2+ affinity, and an increase in cooperativity. NCX affinity for activation by cytoplasmic Ca2+ affinity fell as did the rate of transport. Adding these theoretical capacity curves together also provided a reasonable estimate of total cellular Ca2+ transport (Fig. 9 F). When combined into a mathematical simulation of Ca2+ decay, these fits also replicated the observed time course of Ca2+ clearance in control cells and in 4-blocked cells (Fig. 1 A).

Ca2+ Clearance from the Neurites of Differentiated PC12 Cells

We tried to measure Ca2+ transport in the extensive network of neurites that emerged from our cells upon NGF differentiation. The Ca2+ signals from thin neurites were quite noisy compared with those from the much larger somata because the fluorescence was much dimmer. Therefore, before taking the derivatives of these traces, we fitted the Ca2+ clearance phase for each field of neurites with a double exponential function (Fig. 10 A) and used these functions in our subsequent analysis instead of the original data. Even so, the variability in the Ca2+ transport curves was higher than for somata (Fig. 10 B, black lines) and the results must be regarded as somewhat qualitative. The control transport curve for neurites was statistically indistinguishable from the control curve for somata. The 4-blocked protocol showed that the residual Ca2+ transport of neurites and somata also are similar (Fig. 10 B, gray lines). We next compared the additional BHQ-sensitive transport. As shown in Fig. 10 C, BHQ increased Ca2+ clearance in neurites relative to the 4-blocked protocol. This effect is similar to that seen in undifferentiated PC-12 cells, but unlike that recorded from the somata of differentiated cells (Fig. 10 C). This was the first indication of differences in Ca2+ handling in the neurites versus the somata of differentiated PC12 cells.

The SERCA transport data from 1-blocked experiments with TG-treated neurites clustered into two statistically distinct pools when [Ca2+]cyt exceeded 400 nM. We split these two pools and plot both their ensemble Ca2+ transport curves in Fig. 10 D (gray symbols and lines), along with the somatic Ca2+ capacity curve due to SERCA. According to this dissection, in one population of neurites, Ca2+ is transported by SERCA pumps into the ER, aiding clearance, but in the other Ca2+ emerges from the TG-sensitive ER after depolarization, opposing Ca2+ clearance. Possibly the latter would represent Ca2+-induced Ca2+ release. Neglecting the two pools, the summed TG-sensitive transport in neurites is not statistically different from that in somata. The Ca2+ transport data did not fall into multiple pools in any of the other 1-blocked experiments. For MtU (Fig. 10 E), PMCA (Fig. 10 F), and NCX (Fig. 10 G), overall Ca2+ clearance in neurites was statistically indistinguishable from that in the cell soma, but the power of the test was poor because of the standard errors. Adding the Ca2+ transport curves representing SERCA, MtU, PMCA, NCX, and residual Ca2+ transport together gave a reasonable approximation of total transport in neurites if we chose the SERCA Ca2+ transport curve that shows positive values of clearance (Fig. 10 H). These results show that the Ca2+ transport due to MtU, PMCA, and NCX is similar in cell soma and neurites, but that due to SERCA may vary in different neurites.

Endogenous Ca2+ Binding Ratio of the Cytoplasm in PC12 Cells

Finally, we determined the endogenous Ca2+ binding ratio of PC12 cytoplasm. Only a small fraction of Ca2+ in cells is free; the rest is bound to and buffered by a number of intracellular species. The Ca2+ binding ratio (κ) is the number of bound Ca2+ ions per free Ca2+ ion. To lower the free Ca2+ of a buffered cell by a given amount, the Ca2+ transport machinery of the cell must move 1 + κ times as many Ca2+ ions out of the cytoplasm as it would if there were no buffer. We determined κ for PC12 cells by measuring Ca2+ currents and [Ca2+]cyt simultaneously in cells under the whole-cell configuration. Current measurements give the total amount of Ca2+ that entered the cell (Fig. 11, gray line), and fura-2 measurements give the change in free Ca2+ that occurred as a result (Fig. 11, black line). Using this technique and Eqs. 1 and 2 of Materials and methods, we determined that the endogenous κ in undifferentiated PC12 cells is 268 ± 85 (n = 9).

Using Ca2+ photometry and a pharmacological dissection, we have measured the contributions of several Ca2+ transport mechanisms to Ca2+ dynamics in PC12 cells. In these cells, the two plasma membrane transporters, NCX and PMCA, account for the majority of the clearance from cytoplasm. As [Ca2+]cyt rises above 900 nM, the PMCA tends to saturate, whereas the NCX continues to speed up, becoming dominant at the highest [Ca2+]cyt. This is in accord with the oft-repeated generalization that the PMCA is a high-affinity, low-capacity transporter, whereas the NCX is a low-affinity, high-capacity transporter. The two organellar transporters, SERCA and MtU, contribute less. SERCA pumps account for a small but significant component of clearance, and the MtU contributes little. There is also a residual Ca2+ clearance activity even with our cocktail to block the four canonical mechanisms that is larger than we have seen in other cells. The overall transport remains virtually unchanged by differentiation with NGF. The NCX contribution is lowered, the PMCA contribution is raised, and the apparent Ca2+ affinity decreases for both transporters. In differentiated cells, half of the residual Ca2+ transport is inhibited by addition of BHQ to the 4-block cocktail, suggesting SPCA activity. The NGF-differentiated cells show more Ca2+ influx with depolarization and have a higher dependence on oxidative metabolism than undifferentiated cells. Finally, neurites of differentiated cells show similar overall Ca2+-transporting activity to somata, but the SERCA component and a BHQ-sensitive cellular compartment show differences in detail.

In two cases, we found small differences between the transport capacity curves obtained from 3-blocked experiments and the activity curves obtained from 1-blocked experiments. The capacity curves were slightly higher than the activity curves for the PMCA and NCX in undifferentiated cells (Fig. 2 C and Fig. 3, F and H). A plausible explanation considers possible local Ca2+ gradients. The PMCA and the NCX are the fastest transporters, and they are both in the plasma membrane. When two of them are operating, [Ca2+] near the plasma membrane would be reduced below the mean cytoplasmic level reported by the fura dye, and both mechanisms will be slightly slowed. When one of the two transporters (NCX or PMCA) is inhibited, the other will speed up since the local Ca2+ depletion is reduced. This will have the effect that capacity curves will be overestimated and activity curves will be underestimated. In the Appendix and the online supplemental material we develop calculations showing that the local [Ca2+] at the cell surface can be reduced 7–36% by pumping at the membrane under various assumptions. As described there, the predicted reduction depends strongly on the actual diffusion coefficient for free calcium in the cytoplasm and on the equilibration time for the reaction between Ca2+ and buffering molecules (Fig. S2). Hence, the calculation shows plausibility of the concept but not the exact amount of the effect. This effect, which occurs even without postulating restricted spaces in the cytoplasm, may need to be considered in other transport work.

We saw that the massive Ca2+ loads induced by 30-s depolarizations reduced total clearance rates compared with those after the 3-s depolarizations that we have emphasized in this paper. It is already known that components of transporters can be regulated through changes of phosphorylation state (Toyofuku et al., 1993; Bozulic et al., 2007). The summed model equations we give describe the clearance after 3-s well. We hope that they can be used for other physiological stimulus protocols but have not tested the limits of their use.

Strategies for Ca2+ Clearance in Excitable Cells

We can now compare Ca2+ transport properties of PC12 cells with those of other excitable cells including two rat cells from related catecholaminergic lineages: chromaffin cells (Herrington et al., 1996) and superior cervical ganglion (SCG) neurons (Wanaverbecq et al., 2003). Table II summarizes the overall rates of Ca2+ clearance and the contributions of the four classical mechanisms to clearance measured at 1000 nM [Ca2+]cyt for a variety of excitable cells.

Initial Ca2+ clearance in rat chromaffin cells is dominated by the mitochondria above 500 nM [Ca2+]cyt. The Ca2+ accumulated by mitochondria later returns to the cytoplasm and exits the cell via the slower PMCA and NCX (Herrington et al., 1996). In contrast, in undifferentiated PC12 cells, mitochondria take up little Ca2+ over most of the range of [Ca2+]cyt that we measured (Fig. 3, C and D), and each of the other mechanisms of transport is more than twice as fast as in chromaffin cells at 1000 nM [Ca2+]cyt. Thus the balance of Ca2+ transport mechanisms is remarkably different between undifferentiated PC12 cells and chromaffin cells, although the overall Ca2+ clearance rate during initial clearance is about the same.

NGF-differentiated PC12 cells are a somewhat closer mimic of the Ca2+ transport properties of SCG neurons, but significant differences remain. Both have at most minor mitochondrial transport components. The neurons have almost equal contributions from PMCA, NCX, and SERCA pumps, whereas differentiated PC12 cells have PMCA > NCX > SERCA. It is possible that different isoforms of NCX are present in these different cells and/or that NCX is differentially regulated since the dependence on [Ca2+]cyt looks different. At high values of [Ca2+]cyt, −d[Ca2+]cyt/dt for NCX rises with negative curvature (sublinear) in PC12 cells and with positive curvature (supralinear) in SCG neurons (Wanaverbecq et al., 2003).

Table II includes Ca2+ transport rates for two more cell types, pancreatic β cells and sperm, for comparison. They differ from the others. In β cells the overall transport rate is like the other cells, but SERCA pumps dominate for initial Ca2+ clearance (Chen et al., 2003). In sperm, overall transport is only 1/25 as fast as in the other cells, and SERCA pumps may make no contribution (Wennemuth et al., 2003). We see at least two major lessons from these comparisons. First, PC12 cells and by extension other cancer cells are not quantitative models for the normal differentiated cells whose initial lineage they are thought to share. They may have many of the right molecules and be good systems for study of molecular mechanisms, but they are not close mimics of physiological responses that depend on the quantitative balance of signals. Probably they have gone through many generations of selection as cancer and cultured cells that give them properties molded by a niche quite unlike that of the “parent” cell. That said, PC12 cells do resemble SCG neurons in their mix of Ca2+ transporters. Second, different differentiated cells differ markedly in the balance of Ca2+ transport mechanisms. Presumably the mix of transporters is complementary to the sources of Ca2+ signals in each cell type. Cells that signal largely by Ca2+ entry from the outside eventually will need to clear that Ca2+ to the outside, and cells that signal largely by Ca2+ release from intracellular stores eventually will have to replenish those stores. The number and mix of transporters and the relative importance of organelles vs. the plasma membrane also help to determine whether a Ca2+ signal in that cell is local or widespread, fast or long lasting, large or small, and steady vs. oscillatory. It seems to us that the relation between these factors and cellular physiology still needs much clarification.

Nonmitochondrial Ca2+ Transporters in PC12 Cells

Studies using PC12 cells as a model for neural differentiation have examined the expression patterns of various Ca2+ transporters and how NGF changes them. NGF increases the N- and P/Q-type Ca2+ current (as well as Na+ current) in PC12 cells (Black et al., 2003); Ba2+ currents are tripled. There is an increased transcription of N-type but not of P/Q-type channel genes (Colston et al., 1998). Such observations agree with our finding that NGF differentiation more than doubles the peak [Ca2+]cyt evoked by KCl depolarization (Fig. 6). PC12 cells express SERCA2b and SERCA3 (Rooney and Meldolesi, 1996), the general housekeeping and neuroendocrine-specific mammalian SERCA isoforms, respectively (Wuytack et al., 2002), and NGF is reported to reduce overall SERCA protein levels (Keller and Grover, 2000). This accords with our finding that the SERCA component of transport activity falls a little with differentiation (Table I). PMCAs 1b, 2b, 3a, 3b, 3c, and 4b are present in undifferentiated PC12 cells, and NGF causes up-regulation of PMCAs 1c, 2a, 2c, and 4a (Hammes et al., 1994). Overall, PMCA protein is increased in NGF-differentiated cells (Hammes et al., 1994; Keller and Grover, 2000). This fits with our finding of a 70% increase in PMCA transport rate at 1000 nM Ca2+. Little is known about NCX isoforms in PC12 cells, except that an NCX-mediated Ca2+ influx was observed in Na+-loaded, NGF-differentiated PC12 cells (Ay et al., 2005). The large slowing of Ca2+ clearance upon removal of extracellular Na+ in our experiments indicates that NCX is the most significant component of the Ca2+ transport machinery of undifferentiated PC12 cells and its activity falls a little during NGF differentiation whereas the PMCA rises (Table II).

In summary, the effects of differentiating PC12 cells on Ca2+ transport are subtle. The overall transport rate remains the same. New isoforms of some of the transporters appear and the balance of rates changes a little and the details of [Ca2+] dependence change appreciably (Table I). Presumably some of the changes of gene expression reflect groups of genes regulated by the neural differentiation program. Some of the genes may be essential for differentiation since reducing expression of PMCAs 2 and 3 by antisense methods slows neurite extension and promotes cell death in NGF-treated PC12 cells (Szemraj et al., 2004).

Metabolism in PC12 Cells

A striking difference between the differentiated and undifferentiated cells in our study was that treatment with CCCP slowed PMCA and SERCA pumps in differentiated cells but not in undifferentiated cells. We suggest that the slowing arises because of rapid CCCP-induced ATP depletion in differentiated cells and reflects a switch to dependence on oxidative metabolism in those cells. The published literature supports that interpretation. Treatment of undifferentiated PC12 cells with the protonophore carbonyl cyanide 4-(trifluoromethoxy)phenylhydrazone (FCCP) depletes cellular ATP with a long half-time of 30–100 min (Luo et al., 1997; Kubota et al., 2005), too slow to affect our experiments. This is consistent with the general notion that undifferentiated, rapidly dividing (cancerous) cells rely chiefly on glycolysis for their ATP supply (Racker and Spector, 1981). Treatment with NGF changes metabolism of PC12 cells. After 30 h of NGF, PC12 cells increase glucose consumption threefold, increase lactate and pyruvate production twofold, and increase CO2 production four- to sixfold (Morelli et al., 1986). Levels of all nucleotides including ATP increase, and oxidative respiration increases (Morelli et al., 1986; Davis and Kauffman, 1987). This should make the cells more sensitive to CCCP. They also become more neuron-like in the sense that production of ATP by oxidative phosphorylation and glycolysis occurs in both somata and neurites of sympathetic neurons, with oxidative metabolism predominating (Tolkovsky and Suidan, 1987). Our observation that oligomycin “rescues” the effect of CCCP only a little indicates that ATP pools in the differentiated cells turn over rapidly.

SPCA and Organellar Ca2+ Transport

The results of our experiments using BHQ on top of the 4-block cocktail implicate SPCA as a mechanism of Ca2+ transport in PC12 cells. Although BHQ is also a SERCA inhibitor, cells treated with BHQ were pretreated with TG, so most SERCA activity should have been inhibited already (Wuytack et al., 2002). SPCA was identified as a pump that imports Ca2+ and Mn2+ into the Golgi (Wuytack et al., 2002) and subsequently found immunologically in membrane preparations enriched in dense-core secretory granules (Mitchell et al., 2004). The dense-core secretory granules of insulinoma cells possess a vanadate-sensitive but thapsigargin-insensitive Ca2+-importing ATPase, suggesting a non-SERCA P-type ATPase (Mitchell et al., 2001), which could be SPCA. The transgranular pH gradient should also be considered in our experiments. It might contribute to Ca2+ accumulation by secretory granules and would be collapsed whenever we applied CCCP. While dense core secretory granules can transport Ca2+ in the absence of a pH gradient, the transport can be blunted (Mahapatra et al., 2004). All of our BHQ experiments were conducted in the presence of 2 μM CCCP, which would have collapsed the granular H+ gradient and perhaps led to underestimation of the importance of SPCA-containing compartments to overall Ca2+ dynamics. Our data also do not rule out the possibility that the change in behavior of the BHQ-sensitive compartment(s) due to NGF results from the increased size of the Ca2+ peak in response to depolarization rather than from some molecular change(s) of transporters. Identification of BHQ-sensitive compartments other than the ER and elucidation of the precise nature of the changes that they undergo during NGF differentiation are challenges for the future.

Spatial Differences in Cellular Ca2+ Transport

In differentiated cells, individual dye-filled neurites were too dim to record from reliably. Therefore, we selected fields of view containing up to 20 neurites. The observed Ca2+ transport differed in several ways from that in somata. First, the small BHQ-sensitive Ca2+ transport remaining in neurites of 4-blocked cells had the opposite sign from BHQ-sensitive transport in the soma. Evidently BHQ-sensitive stores take up Ca2+ after depolarization in somata but release Ca2+ in neurites. Perhaps there are different BHQ-sensitive stores in different regions of the cell. Second, we find two behaviors of TG-sensitive stores. In some fields of neurites the ER stores clear Ca2+ and in others they release Ca2+ during clearance. Finally, the dichotomy in SERCA responses is accompanied by an overall excess of variability in the neurites. We feel this is not accounted for fully by the intrinsic noisiness of the neurite data, or by any contamination with soma signals, or by the smaller statistical sample. Rather, we believe that the excess variability reflects a variety of modes of Ca2+ transport present in different neurites.

Conclusions

PC12 cells provide a poor mimic of the balance of Ca2+ transporters observed in primary rat chromaffin cells. Instead, the Ca2+ transporters of both differentiated and undifferentiated PC12 cells more closely resemble those in sympathetic neurons. In particular, a high NCX activity distinguishes Ca2+ clearance in PC12 cells from Ca2+ clearance in chromaffin cells or sympathetic neurons, and the near absence of mitochondrial Ca2+ uptake in PC12 cells is in marked contrast with chromaffin cells where the MtU is by far the dominant transporter. Differentiation with NGF causes some changes in the kinetic details of Ca2+ transport in PC12 cells. Changes in Ca2+ transporter expression conceivably contribute to the neuronal differentiation of PC12 cells. However, although there are switches in transporter isotypes and changes in the observed kinetic constants, there is little net change in total transport. Hence, any effects of these changes must be local rather than global. Indeed, we provided indirect evidence for differences in local gradients at the plasma membrane between undifferentiated and NGF-differentiated cells. Further, undifferentiated PC12 cells are resistant to ATP depletion by CCCP, but differentiated PC12 cells may lose ATP rapidly in response to CCCP; this may lead to rapid inhibition of those Ca2+ transporters that directly consume ATP. A BHQ-sensitive mechanism compatible with SPCA-containing Ca2+ stores apparently provides small contributions to Ca2+ release in undifferentiated cells and to Ca2+ clearance in differentiated cells. As this store could be dense-core secretory granules, it merits further investigation.

Modeling Four Transporters and Residual Transport

We modeled Ca2+ clearance in PC12 cells using mathematical functions for the four canonical Ca2+ transporters that we had used in earlier studies of sperm and pancreatic β-cells (Wennemuth et al., 2003; Chen et al., 2003) plus a function for residual transport. The functions for flux M were:

\[M_{PMCA}{=}M_{\mathrm{max}PMCA}{\times}\frac{1}{1{+}\left(\frac{K_{CaPMCA}}{[Ca^{2{+}}]_{cyt}}\right)^{n_{Hill}}}{\times}\frac{1}{1{+}\left(\frac{K_{aPMCA}}{[H^{{+}}]_{o}}\right)},\]
(3)

where MPMCA is a Hill function of the internal Ca2+ and a saturation function of the external H+. The acid dissociation constant KaPMCA is 13.8 nM, corresponding to pH 7.86.

\begin{eqnarray*}&&M_{NCX}{=}M_{rateNCX}{\times}Allo{\times}\\&&([Na]_{cyt}^{3}[Ca]_{o}e^{{\eta}VF/RT}{-}[Na]_{o}^{3}[Ca]_{cyt}e^{({\eta}{-}1)VF/RT})/Denom\end{eqnarray*}
(4a)
\[Allo{=}1/\left[1{+}\left(\frac{K_{CaAct}}{[Ca]_{cyt}}\right)^{n_{Hill}}\right],\]
(4b)

where MNCX depends on the membrane potential V and the usual thermodynamic constants and Denom is a complex function with many terms (Weber et al., 2001). Values for all parameters except MrateNCX and KCaAct are taken without change from Weber et al. (2001) assuming V = −65 mV.

\[M_{SERCA}{=}M_{\mathrm{max}SERCA}/\left[1{+}\left(\frac{K_{CaSERCA}}{[Ca]_{cyt}}\right)^{n_{Hill}}\right],\]
(5)

where MSERCA is a Hill function of the internal Ca2+.

MMtU is a broken line:

\[\mathrm{For}{\,}[\mathrm{Ca}]_{\mathrm{cyt}}{<}0.3{\,}{\mu}\mathrm{M},{\,}M_{MtU}{=}0\]
(6a)
\[\mathrm{For}{\,}[\mathrm{Ca}]_{\mathrm{cyt}}{<}0.3{\,}{\mu}\mathrm{M},{\,}M_{MtU}{=}Slope_{Mtu}{\times}([Ca]_{cyt}{-}0.3)\]
(6b)
\begin{eqnarray*}&&M_{Residual}{=}M_{\mathrm{maxRe}sidual}/\left[1{+}\left(\frac{K_{Ca\mathrm{Re}sidual}}{[Ca^{2{+}}]_{cyt}}\right)^{2}\right]{+}\\&&Slope_{\mathrm{Re}sidual}{\times}([Ca]_{cyt}{-}1.2),\end{eqnarray*}
(7)

where MResidual is a Hill function of the internal Ca2+ plus a linear rising function that is added in only when internal Ca2+ is >1.2 μM. This purely empirical formulation used the following parameters: MmaxResidual = 15.8 μmol⋅Ca2+ l−1⋅s−1; KCaResidual = 0.7 μM; and SlopeResidual = 44 μmol⋅Ca2+ l−1⋅s−1μM−1.

The equations were entered into the Virtual Cell on-line modeling environment, solved for transport curves (Figs. 2, 4, and 9), and integrated together with the buffer equation (Eq. 2) for simulations of Ca2+ time course during clearance (Fig. 1 A). Parameters for the individual functions (Table II) were optimized by comparing predicted transport curves to measured transport curves for individual mechanisms. The optimization was done by eye, giving no consideration to points >1500 nM Ca2+. The simulated Ca2+ decays in Fig. 1 A are from individual cells rather than from averages, so it was acceptable to scale the transport rates for best agreement. For the control cell the scale factor is 1.0 (no scaling) and for the 4-blocked curve the MResidual was multiplied by 1.65 and the four canonical transporters were multiplied by 0.0 (turned off).

The Virtual Cell model is available in the public domain at http://www.vcell.org/ under the shared username hillelab. The units of transport fluxes there are molecules transported per second per square micrometer and all concentrations are in micromolar. These units were translated into the practical clearance units of Table I by the conversion factors: for PMCA and NCX, 360 Ca2+ ions μm2s−1 = 1 μmol⋅Ca2+ l−1⋅s−1; for SERCA and MtU, 1,851 Ca2+ ions μm2s−1 = 1 μmol⋅Ca2+ l−1⋅s−1

Modeled transport curves were scaled by the same factors and further divided by 1 + κi (Eq. 2) to account for the buffering effects of binding to endogenous buffers and fura.

Modeling Local Gradients

We asked whether transporters acting at the cell surface can deplete local [Ca2+] near the plasma membrane in comparison to the bulk [Ca2+] measured by dyes. The problem was simulated by a multicompartmental planar-diffusion model making many approximations to simplify the calculations. Cytoplasm from cell surface to cell center was represented by a 1-μm-long cylindrical (not conical) column 1 μm2 in cross section and divided into 50 equal thin slices 0.02 μm thick. The first planar compartment was at the cell surface. This model had approximately the same surface-to-volume ratio as our cells. Calcium and a mobile, nonsaturable Ca2+ buffer were allowed to “diffuse” between compartments in successive time increments of 20 ns, using Euler integration. The assumed calcium binding ratio κ for the buffer was 300 and the equilibration time for the reaction of Ca2+ with buffer was varied. The correctness of the equations was verified by showing that a calculation for applying instantaneous point sources of Ca2+ or buffer at the origin resulted in diffusion profiles within 1% of the well-known analytical solution and that mass was conserved within 0.0001%.

The pump problem was simulated by starting with 1 μM Ca2+ throughout the “cell” and continuously removing calcium from the juxta-membrane compartment. According to Table II the PMCA and NCX together reduce free [Ca2+]cyt in PC12 cells at a rate of 423 nM per second at 1000 nM Ca2+. For a cell with κ = 300, this requires removal of 127 μM total calcium per second (see Table I). Our model “cell” starts with 301 μM calcium, free and bound, and the two “pumps” (if linear) would clear the calcium with an appropriate time constant of 301/127 = 2.4 s. As is illustrated in the online supplemental material, activating the “pumps” established a local drop of free [Ca2+]cyt near the membrane that stabilized within a few milliseconds (Fig. S2 A). It extended ∼200 nm into the cytoplasm while the cell center was hardly depleted. The amplitude of the local drop increased if diffusion was slowed (Fig. S2 B), and it increased if the equilibration time of the Ca buffer was slowed (Fig. S2 C). The spatial extent of the gradient decreased when the buffer reaction was speeded. Making the calcium buffer mobile or immobile changed the calculations very little. We express the local drop as a percent of the [Ca2+]cyt at the cell center. For example, with a buffer equilibration time constant of 1 ms, the relative surface drop of free [Ca2+]cyt after 3 ms of pumping was 37%, 23%, and 18% using diffusion coefficients of 0.1, 0.2, and 0.3 × 10−5 cm2/s for free Ca2+ and buffer. Similarly, with a diffusion coefficient of 0.2 × 10−5 cm2/s, the relative surface drop at 3 ms was 39%, 23%, and 8% for buffer equilibration time constants of 10, 1, or 0.1 ms.

We thank Dr. Donner Babcock for his helpful comments on the manuscript. We also thank Lea Miller for technical help and Eric Nguyen for programming.

This work was supported by grants from the National Institutes of Health (NIH), AR17803 (to B. Hille) and F32 DK068982 (to J.G. Duman), the National Science Foundation of China, 30670503 (to L. Chen), and the Major State Basic Research Program of China, 2006CB70570 (to L. Chen). The National Resource for Cell Analysis and Modeling (NRCAM, The Virtual Cell) is supported by the National Center for Research Resources (NCRR) at the NIH.

Olaf S. Andersen served as editor. served as editor.

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J.G. Duman's present address is Department of Neuroscience, Baylor College of Medicine, Houston, TX 77030.

Abbreviations used in this paper: BHQ, tert-butylhydroquinone; GPN, glycyl phenylalanyl β-napthylamide; MtU, mitochondrial Ca2+ uniporter; NCX, Na+-Ca2+ exchanger; NGF, nerve growth factor; PMCA, plasma membrane Ca2+ ATPase; SERCA, sarcoplasmic endoplasmic reticulum Ca2+ ATPase; SPCA, secretory pathway Ca2+ ATPase; TG, thapsigargin.

Supplementary data