A method is described for determining the diffusion coefficient of solutes by determining the rate of passage of the solute through a thin porous membrane between two solutions of different concentration.

The method has been used to determine the diffusion coefficient of carbon monoxide hemoglobin. This was found to be 0.0420 ± 0.0005 cm.2 per day at 5°C.

The molecular weight of carbon monoxide hemoglobin calculated by means of Einstein's equation from this quantity is 68,600 ± 1,000.

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