Volume 139, No. 3, March 5, 2012. Pages 219–234.

In the Results, in the second full paragraph on page 223 (“It was shown that Tricine…”), the sentence “This suggests that excitatory amino acids…” should not have included the word “excitatory.” The correct sentence is “This suggests that amino acids and Tricine could exert their effect on Cav2.3 by chelating trace amounts of Zn2+ and/or Cu2+, which commonly reside in normal bath solutions (Kay, 2004).”

In addition, the footnote b for Table 1 gave an incorrect p-value of P < 0.5. The correct p-value is P < 0.05. The table and corrected footnote are below.

TABLE 1

Biophysical properties

Solution Activation Inactivation kinetics 
Cav2.3 WT PCa(max) ± SEM V1/2 ± SEM k ± SEM n V1/2 ± SEM k ± SEM n Af ± SEM τf ± SEM As ± SEM τs ± SEM n 
HEPES 0.011 ± 0.003 1.5 ± 1.0a 7.2 ± 0.1a 8 −36.1 ± 1.1b 8.9 ± 0.2 8 −0.24 ± 0.05b 0.17 ± 0.02 −0.59 ± 0.04 1.5 ± 0.2 6 
Tricine 0.010 ± 0.002 −15.6 ± 1.8 5.3 ± 0.2 12 −40.2 ± 1.2 8.2 ± 0.3 11 −0.38 ± 0.03 0.13 ± 0.01 −0.44 ± 0.02 1.2 ± 0.1 6 
Zn 7 μM 0.005 ± 0.001 −2.4 ± 2.9a 7.1± 0.1a 4 −30.4 ± 1.8a 9.8 ± 0.5 6 −0.14 ± 0.01a 0.33 ± 0.08 −0.52 ± 0.06 2.4 ± 0.6 6 
Cu 205 nM 0.007 ± 0.002 2.9 ± 1.2a 7.4± 0.1a 6 −26.8 ± 1.2a 9.7 ± 0.8 6 −0.18 ± 0.04a 0.21 ± 0.08 −0.48 ± 0.04 3.1 ± 0.4b 6 
H179E/H181A 
HEPES 0.010 ± 0.002 −12.7 ± 1.0 5.0 ± 0.2 7 −42.9 ± 1.8 9.1 ± 0.4 6 −0.32 ± 0.04 0.14 ± 0.02 −0.59 ± 0.04 1.3 ± 0.1 5 
Tricine 0.010 ± 0.001 −12.1 ± 1.1 5.2 ± 0.2 13 −41.2 ± 1.3 8.4 ± 0.2 8 −0.34 ± 0.03 0.14 ± 0.01 −0.55 ± 0.02 1.2 ± 0.1 5 
Zn 7 μM 0.008 ± 0.002 −9.4 ± 0.9 6.0 ± 0.3 5 −36.0 ± 2.4 9.2 ± 0.8 4 −0.31 ± 0.04 0.23 ± 0.11 −0.65 ± 0.17 2.8 ± 1.1 3 
Cu 205 nM 0.009 ± 0.002 −8.6 ± 1.9 5.6 ± 0.2 8 −37.2 ± 1.1 8.0 ± 0.4 6 −0.33 ± 0.02 0.20 ± 0.06 −0.64 ± 0.12 2.4 ± 0.7 5 
Solution Activation Inactivation kinetics 
Cav2.3 WT PCa(max) ± SEM V1/2 ± SEM k ± SEM n V1/2 ± SEM k ± SEM n Af ± SEM τf ± SEM As ± SEM τs ± SEM n 
HEPES 0.011 ± 0.003 1.5 ± 1.0a 7.2 ± 0.1a 8 −36.1 ± 1.1b 8.9 ± 0.2 8 −0.24 ± 0.05b 0.17 ± 0.02 −0.59 ± 0.04 1.5 ± 0.2 6 
Tricine 0.010 ± 0.002 −15.6 ± 1.8 5.3 ± 0.2 12 −40.2 ± 1.2 8.2 ± 0.3 11 −0.38 ± 0.03 0.13 ± 0.01 −0.44 ± 0.02 1.2 ± 0.1 6 
Zn 7 μM 0.005 ± 0.001 −2.4 ± 2.9a 7.1± 0.1a 4 −30.4 ± 1.8a 9.8 ± 0.5 6 −0.14 ± 0.01a 0.33 ± 0.08 −0.52 ± 0.06 2.4 ± 0.6 6 
Cu 205 nM 0.007 ± 0.002 2.9 ± 1.2a 7.4± 0.1a 6 −26.8 ± 1.2a 9.7 ± 0.8 6 −0.18 ± 0.04a 0.21 ± 0.08 −0.48 ± 0.04 3.1 ± 0.4b 6 
H179E/H181A 
HEPES 0.010 ± 0.002 −12.7 ± 1.0 5.0 ± 0.2 7 −42.9 ± 1.8 9.1 ± 0.4 6 −0.32 ± 0.04 0.14 ± 0.02 −0.59 ± 0.04 1.3 ± 0.1 5 
Tricine 0.010 ± 0.001 −12.1 ± 1.1 5.2 ± 0.2 13 −41.2 ± 1.3 8.4 ± 0.2 8 −0.34 ± 0.03 0.14 ± 0.01 −0.55 ± 0.02 1.2 ± 0.1 5 
Zn 7 μM 0.008 ± 0.002 −9.4 ± 0.9 6.0 ± 0.3 5 −36.0 ± 2.4 9.2 ± 0.8 4 −0.31 ± 0.04 0.23 ± 0.11 −0.65 ± 0.17 2.8 ± 1.1 3 
Cu 205 nM 0.009 ± 0.002 −8.6 ± 1.9 5.6 ± 0.2 8 −37.2 ± 1.1 8.0 ± 0.4 6 −0.33 ± 0.02 0.20 ± 0.06 −0.64 ± 0.12 2.4 ± 0.7 5 

All parameters were calculated for each cell and then averaged. Statistically significant differences are noted with footnotes and are by one-way ANOVA, compared to the values obtained in Tricine:

a

P < 0.001.

b

P < 0.05.

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© 2012 Shcheglovitov et al.
2012