This issue of The Journal includes an important pair of articles (Albrecht et al. 2001; Hongpaisan et al. 2001) that provide a comprehensive analysis of ER contributions to the handling of depolarization-induced Ca2+ loads in bullfrog sympathetic ganglion neurons. The articles include measurements of ER total [Ca] made within individual cisternae identified in cryosections from rapidly frozen cells, using energy-dispersive X-ray microanalysis. Use of the perforated patch recording technique (which avoids the cytosolic dialysis produced by whole-cell recording) allowed an impressive degree of stability in the voltage-clamped currents and fura-2–based measurements of cytosolic [Ca2+] (see Fig. 1 of Albrecht et al. 2001).
Measurements of ER total [Ca] are more direct than the conventional approach of inferring changes in ER [Ca] by measuring how various ER-modifying drugs influence cytosolic [Ca2+] transients. In fact, one of the fundamental contributions of these articles is verification that these pharmacological manipulations actually do (in most cases) produce the predicted changes in basal and stimulated total [Ca] within morphologically identified ER. For example, the relatively high resting value of ER [Ca] (12.8 mmol/kg dry weight, or ∼3.6 mmol/liter hydrated) is shown to be reduced to 5.5 mmol/kg dry weight when ER Ca2+ uptake is inhibited with thapsigargin, and to 4.7 mmol/kg dry weight when ER Ca2+ release channels are opened with a combination of 1 μM ryanodine and 10 mM caffeine.
The articles make another fundamental contribution, namely the documentation of changes in ER total [Ca] in response to the size of the Ca2+ load. Albrecht et al. 2001 present evidence that small Ca2+ loads (produced, e.g., by 2 min in 30 mm K+, average cytosolic [Ca2+] ≤350 nM) are accompanied by both uptake and release of Ca2+ from ER. Since average total ER [Ca] increases from 12.8 to 17.0 mmol/kg dry weight, it is clear that uptake predominates, and that under this condition ER acts as a Ca2+ sink. The authors call this “Mode 1” behavior (Fig. 1). Hongpaisan et al. 2001 present evidence that moderate Ca2+ loads (e.g., 2 min in 50 mM K+, average cytosolic [Ca2+] = 600–800 nM) are also accompanied by both uptake and release; but release does not become regenerative, and there is no significant change in average ER [Ca]. In this situation, termed “Mode 2,” the ER might appear to be just “spinning its wheels”; but, the articles provide evidence for spatial gradients in ER behavior, as discussed below. With higher Ca2+ loads (e.g., 2 min in 50 mM K+ with mitochondrial Ca2+ uptake inhibited, producing peak cytosolic [Ca2+] exceeding 1,000 nM), there was a net loss of Ca from the ER, with total ER [Ca] falling from 12.8 to 6.5 mmol/kg dry weight. In this situation, termed “Mode 3,” ER acts as a Ca2+ source, releasing Ca2+ in a regenerative manner. All of these Ca2+ loads were physiological, in the sense that the “excess” Ca2+ could be extruded completely by the cells. These articles, and the discussion below, do not deal with pathologically high Ca2+ loads such as that depicted in diagram 4 of Fig. 1.
The idea that the net direction of Ca2+ flux across ER varies with stimulus intensity and cytosolic [Ca2+] is not new; but the systematic variation of the Ca2+ load applied to a single neuronal type combined with the linked measurements of total ER [Ca] unite what can be a confusing, fractionated story into a more integrated whole. Albrecht et al. 2001 elaborate a quantitative model that emphasizes graded activation of a single population of Ca2+- and ryanodine-sensitive Ca2+ channels in the ER membrane. Their model reproduces many of the measured effects of ryanodine, caffeine, and thapsigargin on cytosolic [Ca2+] transients and total ER [Ca]. The model applies mainly to Mode 1 conditions, where mitochondrial Ca2+ uptake (which is omitted from the model) is least prominent; but the authors suggest that this model can also account for many of the phenomena measured with the higher Ca2+ loads that evoke Mode 2 and Mode 3 behaviors.
This picture of spatially averaged ER behavior in response to varying Ca2+ loads is complemented by the demonstration of a spatial gradient of ER [Ca] in neurons subjected to a moderate Ca2+ load. ER segments nearest the plasma membrane (and, therefore, presumably exposed to the greatest elevation of cytosolic [Ca2+]) experienced a net Ca loss (Mode 3), whereas ER segments farther from the plasma membrane showed no net change in total [Ca] (Mode 2, Fig. 5 A of Hongpaisan et al. 2001). The behavior of ER segments near the plasma membrane reinforces previous evidence (Hua et al. 1993) that regenerative Ca2+-induced Ca2+ release (CICR, Mode 3 behavior) can occur even in the absence of drugs that alter ER or mitochondrial Ca2+ handling.
In Hongpaisan et al. 2001, measurements of ER total [Ca] are complemented by measurements of total [Ca] within identified mitochondria, based on values from Pivovarova et al. 1999. These measurements present an interesting opportunity to compare the Ca2+ handling behavior of ER and mitochondria. The picture that emerges presents many contrasts. Hongpaisan et al. 2001 report that, under resting physiological conditions, the total [Ca] in ER is almost four times greater than total [Ca] in cytosol (12.8 vs. 3.3 mmol/kg dry weight). In contrast, total [Ca] within the mitochondrial matrix of resting neurons was undetectably low (Pivovarova et al. 1999). ER takes up Ca2+ by active transport (SERCA pumps) and releases it passively via channels sensitive to cytosolic [Ca2+], ryanodine, and caffeine. The uniporter, which transports Ca2+ across in the inner mitochondrial membrane, is also sensitive to cytosolic [Ca2+], but in this organelle, the net direction of passive Ca2+ movement is inward, powered by the sizeable membrane potential (−150 to −200 mV) created by the extrusion of protons via the electron transport chain (for review see Gunter and Pfeiffer 1990). Thus, Ca2+ efflux from mitochondria is the energy-requiring step, mediated via secondary active transport systems (e.g., mitochondrial Na+/Ca2+ exchanger). In bullfrog sympathetic neurons, the net direction of the Ca2+ transport in mitochondria was always inward during high [K+] stimuli, in contrast to the transition from net uptake to net release described above for ER.
The lumena of both organelles have powerful Ca2+ buffering abilities, but measurements of total [Ca] in ER and mitochondria suggest an interesting difference in the modes of Ca2+ storage. Hongpaisan et al. 2001 found that measurements of total [Ca] within the lumen of ER formed a normal distribution under both resting and drug-depleted conditions. This result is consistent with their suggestion that the majority of ER stores in sympathetic neurons are ryanodine-sensitive, and with the idea that Ca (presumably combined with buffers such as calsequestrin) is uniformly distributed within a given ER profile. In contrast, Pivovarova et al. 1999 found marked variability in values of total [Ca] measured in three nonoverlapping regions within the matrix of an individual mitochondrial profile after neuronal depolarization. The measurements of mitochondrial matrix total [Ca] presented in Hongpaisan et al. 2001 used a novel analytical approach (electron energy loss spectrum imaging) that spatially averaged [Ca] over the matrix and, thus, overcame the quantification challenge posed by the intramitochondrial [Ca] heterogeneity. A model proposed by David 1999 suggests that this microheterogeneity is due to punctate, reversible formation of a Ca salt, consistent with his finding that during repetitive stimulation of lizard motor nerve terminals, mitochondrial matrix free [Ca2+] increased to a plateau level (estimated to be ∼1 μM) that was maintained even though mitochondria appeared to continue taking up Ca2+. His data, as well as those presented for hormone-stimulated pituitary gonadotropes by Kaftan et al. 2000, are consistent with the hypothesis that mitochondria are dynamic buffers, whose buffering power increases with increasing Ca2+ uptake. Note, however, that some studies that measured matrix [Ca2+] using mitochondrially targeted aequorins (rather than fluorescent indicator dyes) have reported stimulation-induced elevations of matrix [Ca2+] reaching millimolar levels (e.g., Montero et al. 2000 study in bovine adrenal chromaffin cells).
ER and mitochondrial Ca2+ uptakes have different effects on peak cytosolic [Ca2+] produced during stimulation. The relationship between peak cytosolic [Ca2+] and the size of the Ca2+ load is supralinear under ER-dominant conditions (Hua et al. 1993), but sublinear for mitochondrial-dominant conditions (David et al. 1998). Albrecht et al. 2001 showed that drugs that inhibit Ca2+ uptake into ER (e.g., thapsigargin) altered the time course of small, depolarization-induced cytosolic [Ca2+] transients, but had relatively little effect on peak or final cytosolic [Ca2+]. These observations, taken together with the high resting values of ER total [Ca], are consistent with the idea that the main Ca2+-related task of ER is not to limit the increase in cytosolic [Ca2+] during a sustained Ca2+ challenge, but rather to contribute to intracellular signaling by acting as an “active” filter that dampens the effect of small [Ca2+] transients but amplifies the effect of [Ca2+] transients that exceed a certain threshold. In contrast, depolarizing mitochondria with protonophores (which removes the electrical gradient driving Ca2+ uptake) produced a large increase in the peak amplitude of cytosolic [Ca2+] transients in cells subjected to moderate-to-large Ca2+ loads (Werth and Thayer 1994; Stuenkel 1994; White and Reynolds 1995; Babcock et al. 1997; Sidky and Baimbridge 1997; David et al. 1998, David and Barrett 2000; Hongpaisan et al. 2001), consistent with the idea that a major Ca2+-related task of mitochondria is to limit the peak amplitude of stimulation-induced cytosolic [Ca2+] transients. The passive nature of mitochondrial Ca2+ uptake, combined with their large uptake capacity, may make these organelles ideally suited for rapid sequestration of large Ca2+ loads.
For both ER and mitochondria, stimulation-induced elevations in Ca content decayed slowly (>15 min). On a practical note, this finding suggests that investigators need to use long intertrial intervals if they want to ensure that mitochondrial and ER Ca contents return to resting levels between successive experimental manipulations. A possible functional significance of these long decay times is that stored organellar Ca may contribute to cellular memory mechanisms (Tang and Zucker 1997). As pointed out by Hongpaisan et al. 2001, this type of ER “memory” may be prolonged by mitochondrial Ca2+ extrusion, which (after stimulation) produces a prolonged tail of elevated cytosolic [Ca2+] within the Mode 1 concentration range (200–300 nM) most conducive to ER Ca2+ accumulation (Fig. 1). Additional evidence for mitochondrial influence on ER Ca2+ handling is that disabling mitochondrial Ca2+ uptake with a protonophore (FCCP) changed averaged ER behavior from Mode 2 (little or no net Ca loss) to Mode 3 (significant Ca loss).
Hongpaisan et al. 2001 found an interesting reciprocal relationship between the net Ca uptakes of mitochondria and ER and their distance from the plasma membrane. As noted above, during a moderate Ca2+ load ER profiles near the plasma membrane lost Ca, whereas mitochondria near the membrane gained Ca. The authors mention an interesting question raised by this apparent reciprocity: is the Ca uptake by mitochondria near the plasma membrane influenced by (or might it even require) the loss of Ca from nearby ER, or is this apparent reciprocity due simply to the different but independent responses of these two organelles to the spatial gradient of cytosolic [Ca2+] that bathes them both? Isolated mitochondria require substantial levels (micromolars) of bath [Ca2+] for sustained Ca2+ uptake (Gunter and Pfeiffer 1990), and evidence has been presented that in some preparations the uptake of Ca2+ by mitochondria takes place mainly from highly localized domains of high [Ca2+] found only in the vicinity of open plasma membrane or ER Ca2+ channels (Rizzuto et al. 1999; Szalai et al. 2000). However, a higher affinity, saturable rapid uptake mode has been described in isolated mitochondria (Gunter et al. 1998), and others have found evidence for substantial Ca2+ uptake by mitochondria in neurons and other secretory cells at lower cytosolic [Ca2+] (a few hundred nanomolars) with no required proximity to such channels (Babcock et al. 1997; David et al. 1998; Colegrove et al. 2000). The ability to measure total [Ca] within ER and mitochondria in the same rapidly frozen cells offers the possibility of testing these ideas by determining whether or not the increase in mitochondrial total [Ca] in response to a particular stimulus is substantially reduced by drugs that block ER Ca2+ release.
Another worthwhile effort would be to measure free [Ca2+] and total [Ca] within both ER and mitochondria subjected to the same stimulation patterns. Such measurements would provide insight into the buffering power within the matrix of both organelles. The reversible salt formation model of David 1999 predicts that during sustained stimulation mitochondrial total [Ca] should continue to increase even after matrix free [Ca2+] attains a plateau level. Matched measurements of ER free [Ca2+] and total [Ca] would help answer the question of whether activation of CICR in ER depends mainly on the level of cytosolic [Ca2+] (as in the model of Albrecht et al. 2001) or is also influenced by free [Ca2+] or total [Ca] accumulated within the ER lumen. Similar matched measurements in mitochondria might indicate how opening of the permeability transition pore (which could be viewed as a mitochondrial correlate of CICR) varies with changes in cytosolic and matrix [Ca2+] and total matrix [Ca].