Isolated frog sartorii were immersed in Ringer's solution, which had a 2 mM magnesium concentration containing Mg28. The uptake of the radioactive magnesium was measured under steady state conditions. Although the biological variability was fairly large, it was observed that the uptake proceeded in three stages lasting respectively about 0.5, 30, and 300 minutes and accounting respectively for about 0.21, 0.71, and 0.67 millimole magnesium/kg. muscle. It was assumed that the first stage represents surface adsorption, the second stage represents extracellular water and connective tissue phases, and the third stage entry inside the cell. It is estimated that the maximum intracellular magnesium concentration is about 1.1 mM and that only about 0.6 millimole magnesium/liter intracellular water is exchanged per hour. The maximum energy required per hour to pump the magnesium out of the cell against the electrochemical gradient is calculated to be only 1.5 cal./kg. muscle. About 75 to 80 per cent of the magnesium in muscle is non-exchangeable and difficult to remove by diffusion. It appears from previous work that the exchangeable magnesium behaves similarly to exchangeable calcium.
Skip Nav Destination
Article navigation
1 July 1960
Article|
July 01 1960
Magnesium Equilibrium in Muscle
Daniel L. Gilbert,
Daniel L. Gilbert
From the Department of Physiology, Albany Medical College of Union University, Albany, New York
Search for other works by this author on:
with the technical assistance of JoAnna McGann
with the technical assistance of JoAnna McGann
Search for other works by this author on:
Daniel L. Gilbert
From the Department of Physiology, Albany Medical College of Union University, Albany, New York
with the technical assistance of JoAnna McGann
Received:
December 28 1959
Online ISSN: 1540-7748
Print ISSN: 0022-1295
Copyright, 1960, by The Rockefeller Institute
1960
J Gen Physiol (1960) 43 (6): 1103–1118.
Article history
Received:
December 28 1959
Citation
Daniel L. Gilbert, with the technical assistance of JoAnna McGann; Magnesium Equilibrium in Muscle . J Gen Physiol 1 July 1960; 43 (6): 1103–1118. doi: https://doi.org/10.1085/jgp.43.6.1103
Download citation file:
Sign in
Don't already have an account? Register
Client Account
You could not be signed in. Please check your email address / username and password and try again.
Could not validate captcha. Please try again.
Sign in via your Institution
Sign in via your InstitutionSuggested Content
THE OSMOTIC PRESSURE OF CONCENTRATED SOLUTIONS OF GELATIN IN EQUILIBRIUM WITH SOLUTIONS OF MAGNESIUM CHLORIDE
J Gen Physiol (July,1930)
Axoplasmic free magnesium levels and magnesium extrusion from squid giant axons.
J Gen Physiol (August,1976)
Email alerts
Advertisement