Tissue-resident CSF1R-dependent CD169⁺ macrophages localize deep in the skin. They surround growing tumors and directly suppress their growth independent of T and B cells. CD169⁺ skin macrophages ingest live tumors, and this phagocytosis is independent of the phosphatidylserine receptor MERTK.

This study shows that a secondary microglial expansion center has been identified in fetal brains with oSVZ.

We developed a highly efficient nonviral knock-in platform for NK cells, enabling plasmid-based genome editing with up to ∼90% knock-in efficiency and ∼100% recovery. Targeted integration rewires endogenous circuits for context-dependent therapeutic functions and supports GMP-compatible production of CAR-NK cells for clinical translation applications.

Blanco-Domínguez et al. demonstrate that regulatory T cells constrain IFNγ-producing γδ T cells by competing for IL-2, thereby limiting their expansion and anti-tumor function. IL-2Rβγc agonism can overcome this suppression to enhance γδ T cell–mediated tumor control.

The chemokine CCL25 is expressed in the thymus and the small intestine. Li et al. show that thymic epithelial CCL25 facilitates T cell positive selection in the thymic cortex, whereas intestinal epithelial CCL25 promotes nutrient sensing in the small intestine. Epithelial CCL25 directs thymic T cell development and intestinal homeostasis in a tissue-specific paracrine manner.

CNOT3 promotes the degradation of Tbx21 and Rorc mRNAs through Roquin and ZFP36L1 in ILC2 cells, thereby inhibiting type 1 and type 3 plasticity and maintaining ILC2 function.

Liu et al. develop PRECISE-seq, a single-cell platform linking TCR specificity and avidity to T cell phenotypes in vivo. They reveal that high-potency antiviral T cells become exhausted, while tumor-reactive CD8⁺ T cells acquire a regulatory Ly49⁺ state that is restrained by PD-1 blockade, resulting in effector revival within tumors.