Search Results for cbd

Figure 1. Suppression of inflammatory pain by CBD and DH-CBD in mice. (A) Time course of heat pain hypersensitivity in mice after 20 µl CFA paw injection (1:4 in saline). Each data point represents the mean from 10–15 mice. (B) The analgesic effect of i.p. (50 mg/kg, n = 10) and i.t More about this image found in Suppression of inflammatory pain by CBD and DH-CBD in mice. (A) Time cours...

Figure 5. Antagonism of DD-CBD to DH-CBD–induced potentiation of I_Gly and analgesia in inflammatory pain. (A) Structures of DH-CBD and DD-CBD. Trace records of I_Gly in HEK 293 cells expressing the α3 GlyRs without and with co-application of DD-CBD and DH-CBD. (B) DD-CBD More about this image found in Antagonism of DD-CBD to DH-CBD–induced potentiation of I_Gly and ...

Figure 6. A decrease in CBD and DH-CBD–induced analgesia in the α3^−/− mice but not in the CB1^−/− and CB2 ^−/− mice. (A and B) The analgesic effect of 50 mg/kg CBD i.p. (A) or 50 mg/kg DH-CBD i.p. (B) on PWL to thermal stimulation after CFA injection More about this image found in A decrease in CBD and DH-CBD–induced analgesia in the α3^−/− mice...

Figure 6. HLA-DP2–PLXNA4/Be tetramer staining of ex vivo BAL cells from CBD patients. (A) The binding kinetics of soluble AV22 TCR to biotinylated HLA-DP2–PLXNA4 with and without Be are shown. Four concentrations of soluble AV22 TCR were injected through the flow cells before and after loading More about this image found in HLA-DP2–PLXNA4/Be tetramer staining of ex vivo BAL cells from CBD patients....

Figure 2. DH-CBD potentiation of I_Gly in spinal neurons and suppression of inflammatory pain in rats. (A) Original recordings showing currents activated by puff application of 30 µM glycine to lamina II neurons with and without sustained DH-CBD application in rat spinal cord slices More about this image found in DH-CBD potentiation of I_Gly in spinal neurons and suppression of...

Figure 3. DH-CBD suppression of neuropathic pain in rats. (A) Time courses of the ipsilateral and contralateral PWT to mechanical stimulation after the fifth lumbar SNL in rats (n = 12). ***, P < 0.001, as compared with pre-SNL. (B and C) The analgesic effect of i.t. application More about this image found in DH-CBD suppression of neuropathic pain in rats. (A) Time courses of the ip...

Figure 4. DH-CBD rescue of PGE₂-induced inhibition of the α3 GlyRs and chronic pain. (A) Trace records of I_Gly in HEK 293 cells coexpressing EP2 receptors with either the α3 or α1 GlyRs before and after sustained PGE₂ application. (B) Time courses More about this image found in DH-CBD rescue of PGE₂-induced inhibition of the α3 GlyRs and chr...

Figure 8. NMR analysis: a direct interaction between CBD and S296-containing domain of α3 GlyR. (A) A homology model of α3 GlyR-TM domain structure derived from the high-resolution NMR structure of α1 GlyR TM domains. S296 residue is highlighted in red. (B) Contour plots of the S296 resonance More about this image found in NMR analysis: a direct interaction between CBD and S296-containing domain o...

Figure 9. The effect of DH-CBD on α3 GlyR gating kinetics and agonist affinity. (A) Traces of currents activated by 1 mM glycine in HEK 293 cells expressing WT or S296A mutant α3 GlyRs in the absence and presence of 3 µM DH-CBD. Bar graph shows the mean activation time constant (n = 6 More about this image found in The effect of DH-CBD on α3 GlyR gating kinetics and agonist affinity. (A) ...

Figure 7. Dilated CBD is an early and specific indication of NOD.c3c4 biliary disease and reflects lymphocytic infiltration. (a) Substantially dilated CBDs (CBD) compared with the portal vein (PV) are found only in NOD.c3c4 mice (left) with liver disease, but not in NOD mice (right) or any More about this image found in Dilated CBD is an early and specific indication of NOD.c3c4 biliary disease...

Figure 7. Distribution of plexin A proteins in antigen-presenting cells, BAL fluid, and lung tissue derived from CBD patients. (A) Western blot analysis of cell extracts from the mouse fibroblast cell lines (DP8302 and DP2.21) and EBV-transformed B cells from CBD patient 1332 and BAL fluid from More about this image found in Distribution of plexin A proteins in antigen-presenting cells, BAL fluid, a...