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Journal Articles
The Journal of Experimental Medicine (2020) 217 (4): e20190723.
Published: 24 January 2020
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SD-IVM of the liver allows one to pinpoint the location of platelet clearan...
Published: 24 January 2020
Figure 1. SD-IVM of the liver allows one to pinpoint the location of platelet clearance and to analyze platelet Kupffer cell interactions in the steady state. (A) Schematic depiction of the liver structure (left) and example image of the mouse liver (right) show that crucial components of the l... More
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Following clodronate treatment, Kupffer cells start repopulating the liver ...
Published: 24 January 2020
Figure S1. Following clodronate treatment, Kupffer cells start repopulating the liver from day 7, the number of young platelets decreases while the total platelet count increases and upon desialylation, and CD41-YFP platelets accumulate predominantly on Kupffer cells . (A ) SD-IVM of the mous... More
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Kupffer cells are central to the removal of desialylated platelets under ho...
Published: 24 January 2020
Figure 2. Kupffer cells are central to the removal of desialylated platelets under homeostatic conditions. Mice were Kupffer cell depleted using clodronate liposomes, and the platelet population was followed over time. (A) Representative flow cytometry plots showing a shift in the total platel... More
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Desialylated platelets rapidly accumulate on Kupffer cells.  (A)  Ex vivo d...
Published: 24 January 2020
Figure 3. Desialylated platelets rapidly accumulate on Kupffer cells. (A) Ex vivo desialylated (desial. plts) and untreated control platelets were labeled with CellTracker Green and Red dye, respectively, and transfused into control and Kupffer cell–depleted mice. Representative still images fr... More
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Unaltered Kupffer cell size and recruitment of desialylated platelets but r...
Published: 24 January 2020
Figure S2. Unaltered Kupffer cell size and recruitment of desialylated platelets but reduced fenestrations in mice after arsenic treatment. Mice were treated with arsenic in their drinking water for 5 wk. (A) Quantification of the Kupffer cell size from SD-IVM images. Data represent means ± SE... More
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No accumulation of desialylated platelets in the spleen, lung, or   bone   ...
Published: 24 January 2020
Figure S3. No accumulation of desialylated platelets in the spleen, lung, or bone marrow. (A) Mice were i.v. injected with 50 mU sialidase, and platelet recruitment in the spleen (upper panel) and lung (lower panel) was investigated using SD-IVM. Representative images at the indicated time ... More
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Unaltered Kupffer cell size, number, and function in ST3Gal-IV Δ/Δ  mice.  ...
Published: 24 January 2020
Figure S4. Unaltered Kupffer cell size, number, and function in ST3Gal-IV Δ/Δ mice. (A and B) Kupffer cell (KC) size (A) and total Kupffer cell area per field of view (FOV; B) of wild-type and ST3Gal-IV Δ/Δ mice were quantified from SD-IVM images. (C) Kupffer cell function in wild-type and ... More
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Desialylated platelets are phagocytosed   by Kupffer cells   in vitro, Mac1...
Published: 24 January 2020
Figure S5. Desialylated platelets are phagocytosed by Kupffer cells in vitro, Mac1 is dispensable for the clearance of desialylated platelets, Kupffer cells express Asgr1 and MGL1/2, but Asgr1-deficiency alone has no effect on platelet count . (A) Mice were treated with 50 mU sialidase i.v... More
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Kupffer cells phagocytose desialylated platelets.  (A)  SD-IVM of the liver...
Published: 24 January 2020
Figure 4. Kupffer cells phagocytose desialylated platelets. (A) SD-IVM of the liver of wild-type mice i.v. injected with desialylated platelets labeled with CellTracker Red dye. Representative images taken at the indicated time points. Kupffer cells were labeled using an anti-F4/80 antibody. No... More
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A collaboration between MGL and AMR facilitates binding of desialylated pla...
Published: 24 January 2020
Figure 5. A collaboration between MGL and AMR facilitates binding of desialylated platelets to Kupffer cells. (A) SD-IVM was performed on the liver of control mice, mice treated with ASF, Asgr1 −/− mice, wild-type mice treated with MGL1/2 blocking antibody (Ab), and Asgr1 −/− mice treated... More
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Old platelets show significantly reduced hemostatic capacity.  (A and B)  F...
Published: 24 January 2020
Figure 6. Old platelets show significantly reduced hemostatic capacity. (A and B) Flow cytometric analysis of platelets from mice 5 d after Kupffer cell depletion shows increased P-selectin exposure in resting conditions, but decreased P-selectin exposure after stimulation with thrombin (Thr) c... More
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Insights from Miwa Sasai (left) and Masahiro Yamamoto (right).
Published: 22 January 2020
Insights from Miwa Sasai (left) and Masahiro Yamamoto (right). More
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T    . gondii      injects ROP16 to polarize macrophage toward M2 macrophag...
Published: 22 January 2020
T . gondii injects ROP16 to polarize macrophage toward M2 macrophage without infection. After attachment to the host cell, T. gondii injects rhoptry contents with or without invasion. ROP16 is also injected to activate host transcription factors STAT3 and STAT6, resulting in expression of... More
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Plaque-associated myeloid cells are resident, yolk sac–derived microglia.  ...
Published: 22 January 2020
Figure 1. Plaque-associated myeloid cells are resident, yolk sac–derived microglia. (A) Schematic for tamoxifen exposure to drive expression of tdTomato in Cx3cr1 CreERT2 ; 5xFAD; ROSA26 tdTomato (top, purple) or Ccr2 CreERT2 ; 5xFAD; ROSA26 tdTomato (bottom, yellow) mice. Tamoxifen treat... More
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Kinetics of labeled peripheral monocytes using  Ccr2-CreERT2 .  The fractio...
Published: 22 January 2020
Figure S1. Kinetics of labeled peripheral monocytes using Ccr2-CreERT2 . The fraction of monocytes (MOs; CD11b + CD115 + ), neutrophils (NPs; CD11b + Ly6G + ), and dendritic cells (DCs; CD11b + CD11c + MHCII + ) expressing tdTomato immediately after 5 d of tamoxifen-containing chow, or followin... More
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Labeling of peripheral monocyte populations following tamoxifen exposure.  ...
Published: 22 January 2020
Figure 2. Labeling of peripheral monocyte populations following tamoxifen exposure. (A) Representative flow sorting scheme for tdTomato expression in Ly6C hi and Ly6C lo monocytes from the spleen. FSC, forward scatter; SSC, side scatter. (B and C) Fraction of Ly6C hi and Ly6C lo monocytes... More
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An in vitro priming approach to generate functional pathogen-specific Th ce...
Published: 22 January 2020
Figure S1. An in vitro priming approach to generate functional pathogen-specific Th cells. (A) Top: Purity of isolated naive CD4 T cells (CD4 + MHCII − CD62L + CD44 − ) from two independent experiments. Bottom: Purity of CD11c + DCs before or after isolation. (B) Overlay histograms of CFSE d... More
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Validation of an in vitro–priming approach to generate functional pathogen-...
Published: 22 January 2020
Figure 1. Validation of an in vitro–priming approach to generate functional pathogen-specific Th cells. (A) Schematic overview of the priming system and workflow. (B) Top row: Representative CFSE dilution graph and cytokine (IFNγ and IL-17A) staining from CFSE + and CFSE − populations. Bott... More
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Comparative transcriptional analysis reveals caspase-1 as a T cell–intrinsi...
Published: 22 January 2020
Figure S2. Comparative transcriptional analysis reveals caspase-1 as a T cell–intrinsic regulator of Th17 differentiation. (A) tdT + percentage of unstimulated (Un), Lm-primed, Cr-primed, or Th17-polarized 17A-fm CD4 T cells. Un, n = 4; Lm and Cr, n = 5; Th17, n = 2. (B) Heatmap of Th1... More