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Journal Articles
The Journal of Experimental Medicine (2020) 217 (4): e20191869.
Published: 17 January 2020
Includes: Supplementary data
Images
Omentum is a critical premetastatic niche for ovarian cancer cells.  (A)  L...
Published: 17 January 2020
Figure 1. Omentum is a critical premetastatic niche for ovarian cancer cells. (A) Location of the omentum in the abdominal cavity of mice. (B) In vivo bioluminescence imaging of mice at 1, 21, 35, and 63 d after i.p. injection of 10 6 ID8-Luc cells; scale bar: 1 cm. (C) H&E stain of fr... More
Images
Single-cell transcriptional profiling of omental macrophages after tumor co...
Published: 17 January 2020
Figure 2. Single-cell transcriptional profiling of omental macrophages after tumor colonization. (A) Whole-mount confocal imaging of omentum showing the location of FALCs by visualizing collagen IV (ColIV, green), adipocytes (LipidTox, red), and leukocyte clusters (CD45, white); scale bar: 0.5 ... More
Images
Characterization of macrophage subsets in omentum by flow cytometry and con...
Published: 17 January 2020
Figure 3. Characterization of macrophage subsets in omentum by flow cytometry and confocal microscopy. (A) Flow cytometry analysis of the myeloid cell compartment in omentum. Myeloid cells are gated as (i) Live, CD45.2 + , singlets, Lin neg (CD5, CD19, NK1.1, Ly6G, Siglec F), CD11b + , and sub... More
Images
Gating strategy and expression of Tim4 and CD163 on omental macrophages  . ...
Published: 17 January 2020
Figure S1. Gating strategy and expression of Tim4 and CD163 on omental macrophages . (A ) Gating strategy for the myeloid cell compartment excluding neutrophils. Myeloid cells were gated as Live (forward scatter area [FSC-A] versus Sytox Blue), CD45.2 + (CD45.2 versus SSC-A), CD5 − , CD19 − ... More
Images
Embryonic origin of tissue-resident CD163 +  Tim4 +  macrophages in omentum...
Published: 17 January 2020
Figure 4. Embryonic origin of tissue-resident CD163 + Tim4 + macrophages in omentum. (A) Analysis of macrophage ontogeny in ID8 tumor-bearing mice using shielded chimeras; host CD45.1 congenic mice were placed in a protective lead shield with only the hind legs exposed, before irradiation at ... More
Images
Fate mapping of omental macrophages  .  (A and B)  Flow cytometric analysis...
Published: 17 January 2020
Figure S2. Fate mapping of omental macrophages . (A and B) Flow cytometric analysis of blood monocytes in mice 5 wk (A) or 13 wk (B) after adoptive transfer of CD45.1 + /CD45.2 + donor bone marrow in irradiated CD45.1 + host mice that were protected by a lead shield. Monocytes were gated as ... More
Images
CD163 +  Tim4 +  tissue-resident macrophages express a unique transcription...
Published: 17 January 2020
Figure 5. CD163 + Tim4 + tissue-resident macrophages express a unique transcriptional profile. (A) Gating strategy for cell sorting of P1, P2, and P3 populations of omental macrophages from naive ( n = 2) and tumor-bearing mice at 5 and 10 wk after injection of ID8 cells ( n = 4). 500 cells... More
Images
Hierarchical clustering analysis of omental macrophages  .  (A)  Heatmap sh...
Published: 17 January 2020
Figure S3. Hierarchical clustering analysis of omental macrophages . (A) Heatmap showing hierarchical clustering analysis of the 5,000 most DEGs in P1 (green), P2 (red), and P3 (blue) CD169 hi Lyve-1 pos omental macrophages sorted at steady state (0 wk) or 5 wk and 10 wk after infiltration o... More
Images
Depletion of monocyte-derived and tissue-resident omental macrophages  .  (...
Published: 17 January 2020
Figure S4. Depletion of monocyte-derived and tissue-resident omental macrophages . (A and B) Flow cytometric analysis of CD169 hi Lyve-1 pos omental macrophages in Ccr2 −/− mice 10 wk after i.p. injection of 106 ID8 ovarian cancer cells. Data are represented as mean ± SEM of n = 5, and ... More
Images
Specific depletion of CD163 +  Tim4 +  tissue-resident macrophages prevents...
Published: 17 January 2020
Figure 6. Specific depletion of CD163 + Tim4 + tissue-resident macrophages prevents metastatic spread of ovarian cancer. (A) Cohorts of Cd163-Csf1r DTR or control mice ( Csf1r LSL-DTR ) were injected with 4 ng/kg DT 6 d before transplantation with ID8 cells. Mice were analyzed for depleti... More
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Transcriptomic analysis of ID8 ovarian cancer cells and TAMs from omentum  ...
Published: 17 January 2020
Figure S5. Transcriptomic analysis of ID8 ovarian cancer cells and TAMs from omentum . (A – D) Heatmaps showing expression of genes associated with the enriched pathways in Fig. 5 E : Positive regulation of JAK-STAT (A), angiogenesis (B), regulation of tissue remodeling (C), and T cell diff... More
Images
Ovarian cancer cells in ascites acquire CSC characteristics.  (A)  Heatmap ...
Published: 17 January 2020
Figure 7. Ovarian cancer cells in ascites acquire CSC characteristics. (A) Heatmap showing expression of CSC markers in omental ID8 cells (ID8-OM) and ID8 cells from ascites (ID8-A11); see Table S1 for details. (B) Flow cytometry analysis of CSC surface markers on ascitic ID8-A11 cells comp... More
Images
CD163 +  Tim4 +  tissue-resident macrophages promote the CSC-like phenotype...
Published: 17 January 2020
Figure 8. CD163 + Tim4 + tissue-resident macrophages promote the CSC-like phenotype of ovarian cancer cells. (A) Specific depletion of CD163 + Tim4 + (P4) macrophages using dxr-loaded αCD163-LNPs (αCD163-dxr); mice were injected i.p. with 1 mg/kg αCD163-dxr or controls (empty αCD163-LNPs or... More
Journal Articles
The Journal of Experimental Medicine (2020) 217 (4): e20190762.
Published: 16 January 2020
Includes: Supplementary data
Images
IL-34 can bind three different receptors.  In addition to CSF-1R (CD115), w...
Published: 15 January 2020
Figure 1. IL-34 can bind three different receptors. In addition to CSF-1R (CD115), which is the main receptor for IL-34, PTP-ζ and syndecan-1 (CD138) have recently been identified as alternative receptors ( Nandi et al., 2013 ; Ségaliny et al., 2015b ). IL-34–mediated receptor activation can in... More
Images
Tissue expression of IL-34 in the steady state. (A)  Shown are tissues and ...
Published: 15 January 2020
Figure 2. Tissue expression of IL-34 in the steady state. (A) Shown are tissues and cell types expressing IL-34 under physiological conditions ( Lin et al., 2008 ; Tabula Muris Consortium, 2018 ). In the brain, IL-34 is produced by neurons, which control microglial homeostasis in adulthood ( Gr... More
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Protective and disease-promoting roles of IL-34 in disease.  High expressio...
Published: 15 January 2020
Figure 3. Protective and disease-promoting roles of IL-34 in disease. High expression of IL-34 correlates with disease severity in autoimmune diseases (Sjögren’s syndrome [ Ciccia et al., 2013 ; Fox, 2005 ], SLE [ Wang et al., 2016b ; Xie et al., 2018 ], psoriasis [ Mizuno et al., 2011 ], and ... More
Images
MHC-I immunogenicity of neoantigen candidates.  (A)  Number of exome and tr...
Published: 15 January 2020
Figure 1. MHC-I immunogenicity of neoantigen candidates. (A) Number of exome and transcript variations, predicted peptides, and detected CD8 T cell responses for four mouse tumor cell lines (MC-38, TRAMP-C1, EMT-6, and CT-26). (B) Schema depicting the study protocol. Naive mice were immunized... More
Images
Validation of MHC-I optimal predicted mutant epitopes.  (A–C)  Representati...
Published: 15 January 2020
Figure 2. Validation of MHC-I optimal predicted mutant epitopes. (A–C) Representative pictures of ELISpot wells and mean (± SD) IFN-gamma spot numbers from CD8 T cells (5 × 10 5 CD4-depleted splenocytes/well) from control (Ctr., n = 1) or immunized (Im., n = 3) mice (in vivo protocol as in... More