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1-17 of 17
Hedda Wardemann
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Journal Articles
In Special Collection:
Antibodies at the Forefront of Disease Treatment
Elaine Thai, Giulia Costa, Anna Weyrich, Rajagopal Murugan, David Oyen, Yevel Flores-Garcia, Katherine Prieto, Alexandre Bosch, Angelo Valleriani, Nicholas C. Wu, Tossapol Pholcharee, Stephen W. Scally, Ian A. Wilson, Hedda Wardemann, Jean-Philippe Julien, Elena A. Levashina
Journal:
Journal of Experimental Medicine
Journal of Experimental Medicine (2020) 217 (11): e20200061.
Published: 13 August 2020
Abstract
Malaria is a global health concern, and research efforts are ongoing to develop a superior vaccine to RTS,S/AS01. To guide immunogen design, we seek a comprehensive understanding of the protective humoral response against Plasmodium falciparum (Pf) circumsporozoite protein (PfCSP). In contrast to the well-studied responses to the repeat region and the C-terminus, the antibody response against the N-terminal domain of PfCSP (N-CSP) remains obscure. Here, we characterized the molecular recognition and functional efficacy of the N-CSP–specific monoclonal antibody 5D5. The crystal structure at 1.85-Å resolution revealed that 5D5 binds an α-helical epitope in N-CSP with high affinity through extensive shape and charge complementarity and the unusual utilization of an antibody N-linked glycan. Nevertheless, functional studies indicated low 5D5 binding to live Pf sporozoites and lack of sporozoite inhibition in vitro and in vivo. Overall, our data do not support the inclusion of the 5D5 N-CSP epitope into the next generation of CSP-based vaccines.
Includes: Supplementary data
Journal Articles
Johanna Kabbert, Julia Benckert, Tim Rollenske, Thomas C.A. Hitch, Thomas Clavel, Vuk Cerovic, Hedda Wardemann, Oliver Pabst
Journal:
Journal of Experimental Medicine
Journal of Experimental Medicine (2020) 217 (11): e20200275.
Published: 08 July 2020
Abstract
The gut is home to the body’s largest population of plasma cells. In healthy individuals, IgA is the dominating isotype, whereas patients with inflammatory bowel disease also produce high concentrations of IgG. In the gut lumen, secretory IgA binds pathogens and toxins but also the microbiota. However, the antigen specificity of IgA and IgG for the microbiota and underlying mechanisms of antibody binding to bacteria are largely unknown. Here we show that microbiota binding is a defining property of human intestinal antibodies in both healthy and inflamed gut. Some bacterial taxa were commonly targeted by different monoclonal antibodies, whereas others selectively bound single antibodies. Interestingly, individual human monoclonal antibodies from both healthy and inflamed intestines bound phylogenetically unrelated bacterial species. This microbiota cross-species reactivity did not correlate with antibody polyreactivity but was crucially dependent on the accumulation of somatic mutations. Therefore, our data suggest that a system of affinity-matured, microbiota cross-species–reactive IgA is a common aspect of SIgA–microbiota interactions in the gut.
Includes: Supplementary data
Journal Articles
Delphine Sterlin, Jehane Fadlallah, Olivia Adams, Claire Fieschi, Christophe Parizot, Karim Dorgham, Asok Rajkumar, Gaëlle Autaa, Hela El-Kafsi, Jean-Luc Charuel, Catherine Juste, Friederike Jönsson, Thomas Candela, Hedda Wardemann, Alexandra Aubry, Carmen Capito, Hélène Brisson, Christophe Tresallet, Richard D. Cummings, Martin Larsen, Hans Yssel, Stephan von Gunten, Guy Gorochov
Journal:
Journal of Experimental Medicine
Journal of Experimental Medicine (2020) 217 (3): e2018163501152020c.
Published: 28 January 2020
Journal Articles
In Special Collection:
Immunology: New Insights and Resources
,
Tissue immune responses: immune cells and mechanisms
Delphine Sterlin, Jehane Fadlallah, Olivia Adams, Claire Fieschi, Christophe Parizot, Karim Dorgham, Asok Rajkumar, Gaëlle Autaa, Hela El-Kafsi, Jean-Luc Charuel, Catherine Juste, Friederike Jönsson, Thomas Candela, Hedda Wardemann, Alexandra Aubry, Carmen Capito, Hélène Brisson, Christophe Tresallet, Richard D. Cummings, Martin Larsen, Hans Yssel, Stephan von Gunten, Guy Gorochov
Journal:
Journal of Experimental Medicine
Journal of Experimental Medicine (2019) 217 (3): e20181635.
Published: 31 December 2019
Abstract
In humans, several grams of IgA are secreted every day in the intestinal lumen. While only one IgA isotype exists in mice, humans secrete IgA1 and IgA2, whose respective relations with the microbiota remain elusive. We compared the binding patterns of both polyclonal IgA subclasses to commensals and glycan arrays and determined the reactivity profile of native human monoclonal IgA antibodies. While most commensals are dually targeted by IgA1 and IgA2 in the small intestine, IgA1 + IgA2 + and IgA1 − IgA2 + bacteria coexist in the colon lumen, where Bacteroidetes is preferentially targeted by IgA2. We also observed that galactose-α terminated glycans are almost exclusively recognized by IgA2. Although bearing signs of affinity maturation, gut-derived IgA monoclonal antibodies are cross-reactive in the sense that they bind to multiple bacterial targets. Private anticarbohydrate-binding patterns, observed at clonal level as well, could explain these apparently opposing features of IgA, being at the same time cross-reactive and selective in its interactions with the microbiota.
Includes: Supplementary data
Journal Articles
Khaled Amara, Johanna Steen, Fiona Murray, Henner Morbach, Blanca M. Fernandez-Rodriguez, Vijay Joshua, Marianne Engström, Omri Snir, Lena Israelsson, Anca I. Catrina, Hedda Wardemann, Davide Corti, Eric Meffre, Lars Klareskog, Vivianne Malmström
Journal:
Journal of Experimental Medicine
Journal of Experimental Medicine (2018) 216 (1): 245.
Published: 19 December 2018
Journal Articles
Stephen W. Scally, Rajagopal Murugan, Alexandre Bosch, Gianna Triller, Giulia Costa, Benjamin Mordmüller, Peter G. Kremsner, B. Kim Lee Sim, Stephen L. Hoffman, Elena A. Levashina, Hedda Wardemann, Jean-Philippe Julien
Journal:
Journal of Experimental Medicine
Journal of Experimental Medicine (2017) 215 (1): 63–75.
Published: 22 November 2017
Abstract
Antibodies against the central repeat of the Plasmodium falciparum (Pf) circumsporozoite protein (CSP) inhibit parasite activity and correlate with protection from malaria. However, the humoral response to the PfCSP C terminus (C-PfCSP) is less well characterized. Here, we describe B cell responses to C-PfCSP from European donors who underwent immunization with live Pf sporozoites (PfSPZ Challenge) under chloroquine prophylaxis (PfSPZ-CVac), and were protected against controlled human malaria infection. Out of 215 PfCSP-reactive monoclonal antibodies, only two unique antibodies were specific for C-PfCSP, highlighting the rare occurrence of C-PfCSP–reactive B cells in PfSPZ-CVac–induced protective immunity. These two antibodies showed poor sporozoite binding and weak inhibition of parasite traversal and development, and did not protect mice from infection with PfCSP transgenic Plasmodium berghei sporozoites. Structural analyses demonstrated that one antibody interacts with a polymorphic region overlapping two T cell epitopes, suggesting that variability in C-PfCSP may benefit parasite escape from humoral and cellular immunity. Our data identify important features underlying C-PfCSP shortcomings as a vaccine target.
Includes: Supplementary data
Journal Articles
Khaled Amara, Johanna Steen, Fiona Murray, Henner Morbach, Blanca M. Fernandez-Rodriguez, Vijay Joshua, Marianne Engström, Omri Snir, Lena Israelsson, Anca I. Catrina, Hedda Wardemann, Davide Corti, Eric Meffre, Lars Klareskog, Vivianne Malmström
Journal:
Journal of Experimental Medicine
Journal of Experimental Medicine (2013) 210 (3): 445–455.
Published: 25 February 2013
Abstract
Antibodies targeting citrullinated proteins (ACPAs [anticitrullinated protein antibodies]) are commonly found in patients with rheumatoid arthritis (RA), strongly associate with distinct HLA-DR alleles, and predict a more aggressive disease course as compared with seronegative patients. Still, many features of these antibodies, including their site of production and the extent of MHC class II–driven T cell help, remain unclarified. To address these questions, we have used a single B cell–based cloning technology to isolate and express immunoglobulin (Ig) genes from joint-derived B cells of active RA patients. We found ∼25% of synovial IgG-expressing B cells to be specific for citrullinated autoantigens in the investigated ACPA + RA patients, whereas such antibodies were not found in ACPA − patients. The citrulline-reactive monoclonal antibodies did not react with the unmodified arginine peptides, yet several reacted with more than one citrullinated antigen. A role for active antigen selection of the citrulline-reactive synovial B cells was supported by the strong bias toward amino acid replacement mutations in ACPA + antibodies and by their loss of reactivity to citrullinated autoantigens when somatic mutations were reverted to the corresponding germline sequences.
Includes: Supplementary data
Journal Articles
Matthias F. Muellenbeck, Beatrix Ueberheide, Borko Amulic, Alexandra Epp, David Fenyo, Christian E. Busse, Meral Esen, Michael Theisen, Benjamin Mordmüller, Hedda Wardemann
Journal:
Journal of Experimental Medicine
Journal of Experimental Medicine (2013) 210 (2): 389–399.
Published: 14 January 2013
Abstract
Antibodies can protect from Plasmodium falciparum ( Pf ) infection and clinical malaria disease. However, in the absence of constant reexposure, serum immunoglobulin (Ig) levels rapidly decline and full protection from clinical symptoms is lost, suggesting that B cell memory is functionally impaired. We show at the single cell level that natural Pf infection induces the development of classical memory B cells (CM) and atypical memory B cells (AtM) that produce broadly neutralizing antibodies against blood stage Pf parasites. CM and AtM contribute to anti- Pf serum IgG production, but only AtM show signs of active antibody secretion. AtM and CM were also different in their IgG gene repertoire, suggesting that they develop from different precursors. The findings provide direct evidence that natural Pf infection leads to the development of protective memory B cell antibody responses and suggest that constant immune activation rather than impaired memory function leads to the accumulation of AtM in malaria. Understanding the memory B cell response to natural Pf infection may be key to the development of a malaria vaccine that induces long-lived protection.
Includes: Supplementary data
Journal Articles
Thomas Tiller, Juliane Kofer, Cornelia Kreschel, Christian E. Busse, Stefan Riebel, Susanne Wickert, Felix Oden, Maria M.M. Mertes, Marc Ehlers, Hedda Wardemann
Journal:
Journal of Experimental Medicine
Journal of Experimental Medicine (2010) 207 (12): 2767–2778.
Published: 15 November 2010
Abstract
Abnormalities in expression levels of the IgG inhibitory Fc gamma receptor IIB (FcγRIIB) are associated with the development of immunoglobulin (Ig) G serum autoantibodies and systemic autoimmunity in mice and humans. We used Ig gene cloning from single isolated B cells to examine the checkpoints that regulate development of autoreactive germinal center (GC) B cells and plasma cells in FcγRIIB-deficient mice. We found that loss of FcγRIIB was associated with an increase in poly- and autoreactive IgG + GC B cells, including hallmark anti-nuclear antibody–expressing cells that possess characteristic Ig gene features and cells producing kidney-reactive autoantibodies. In the absence of FcγRIIB, autoreactive B cells actively participated in GC reactions and somatic mutations contributed to the generation of highly autoreactive IgG antibodies. In contrast, the frequency of autoreactive IgG + B cells was much lower in spleen and bone marrow plasma cells, suggesting the existence of an FcγRIIB-independent checkpoint for autoreactivity between the GC and the plasma cell compartment.
Includes: Supplementary data
Journal Articles
Barbara Cassani, Pietro Luigi Poliani, Veronica Marrella, Francesca Schena, Aisha V. Sauer, Maria Ravanini, Dario Strina, Christian E. Busse, Stephan Regenass, Hedda Wardemann, Alberto Martini, Fabio Facchetti, Mirjam van der Burg, Antonius G. Rolink, Paolo Vezzoni, Fabio Grassi, Elisabetta Traggiai, Anna Villa
Journal:
Journal of Experimental Medicine
Journal of Experimental Medicine (2010) 207 (7): 1525–1540.
Published: 14 June 2010
Abstract
Hypomorphic RAG mutations, leading to limited V(D)J rearrangements, cause Omenn syndrome (OS), a peculiar severe combined immunodeficiency associated with autoimmune-like manifestations. Whether B cells play a role in OS pathogenesis is so far unexplored. Here we report the detection of plasma cells in lymphoid organs of OS patients, in which circulating B cells are undetectable. Hypomorphic Rag2 R229Q knock-in mice, which recapitulate OS, revealed, beyond severe B cell developmental arrest, a normal or even enlarged compartment of immunoglobulin-secreting cells (ISC). The size of this ISC compartment correlated with increased expression of Blimp1 and Xbp1, and these ISC were sustained by elevated levels of T cell derived homeostatic and effector cytokines. The detection of high affinity pathogenic autoantibodies toward target organs indicated defaults in B cell selection and tolerance induction. We hypothesize that impaired B cell receptor (BCR) editing and a serum B cell activating factor (BAFF) abundance might contribute toward the development of a pathogenic B cell repertoire in hypomorphic Rag2 R229Q knock-in mice. BAFF-R blockade reduced serum levels of nucleic acid-specific autoantibodies and significantly ameliorated inflammatory tissue damage. These findings highlight a role for B cells in OS pathogenesis.
Includes: Supplementary data
Journal Articles
Sergey Yurasov, Thomas Tiller, Makoto Tsuiji, Klara Velinzon, Virginia Pascual, Hedda Wardemann, Michel C. Nussenzweig
Journal:
Journal of Experimental Medicine
Journal of Experimental Medicine (2006) 203 (10): 2255–2261.
Published: 11 September 2006
Abstract
A majority of the antibodies expressed by nascent B cells in healthy humans are self-reactive, but most of these antibodies are removed from the repertoire during B cell development. In contrast, untreated systemic lupus erythematosus (SLE) patients fail to remove many of the self-reactive and polyreactive antibodies from the naive repertoire. Here, we report that SLE patients in clinical remission continue to produce elevated numbers of self-reactive and polyreactive antibodies in the mature naive B cell compartment, but the number of B cells expressing these antibodies is lower than in patients with active disease. Our finding that abnormal levels of self-reactive mature naive B cells persist in the majority of patients in clinical remission suggests that early checkpoint abnormalities are an integral feature of SLE.
Includes: Supplementary data
Journal Articles
Makoto Tsuiji, Sergey Yurasov, Klara Velinzon, Saskia Thomas, Michel C. Nussenzweig, Hedda Wardemann
Journal:
Journal of Experimental Medicine
Journal of Experimental Medicine (2006) 203 (2): 393–400.
Published: 30 January 2006
Abstract
Autoantibodies are removed from the repertoire at two checkpoints during B cell development in the bone marrow and the periphery. Despite these checkpoints, up to 20% of the antibodies expressed by mature naive B cells in healthy humans show low levels of self-reactivity. To determine whether self-reactive antibodies are also part of the antigen-experienced memory B cell compartment, we analyzed recombinant antibodies cloned from single circulating human IgM + memory B cells. Cells expressing antibodies specific for individual bacterial polysaccharides were expanded in the IgM + memory compartment. In contrast, B cells expressing self-reactive and broadly bacterially reactive antibodies were removed from the repertoire in the transition from naive to IgM + memory B cell. Selection against self-reactive antibodies was implemented before the onset of somatic hypermutation. We conclude that a third checkpoint selects against self-reactivity during IgM + memory B cell development in humans.
Includes: Supplementary data
Journal Articles
Sergey Yurasov, Hedda Wardemann, Johanna Hammersen, Makoto Tsuiji, Eric Meffre, Virginia Pascual, Michel C. Nussenzweig
Journal:
Journal of Experimental Medicine
Journal of Experimental Medicine (2005) 201 (5): 703–711.
Published: 28 February 2005
Abstract
A cardinal feature of systemic lupus erythematosus (SLE) is the development of autoantibodies. The first autoantibodies described in patients with SLE were those specific for nuclei and DNA, but subsequent work has shown that individuals with this disease produce a panoply of different autoantibodies. Thus, one of the constant features of SLE is a profound breakdown in tolerance in the antibody system. The appearance of self-reactive antibodies in SLE precedes clinical disease, but where in the B cell pathway tolerance is first broken has not been defined. In healthy humans, autoantibodies are removed from the B cell repertoire in two discrete early checkpoints in B cell development. We found these checkpoints to be defective in three adolescent patients with SLE. 25–50% of the mature naive B cells in SLE patients produce self-reactive antibodies even before they participate in immune responses as compared with 5–20% in controls. We conclude that SLE is associated with abnormal early B cell tolerance.
Includes: Supplementary data
Journal Articles
Journal:
Journal of Experimental Medicine
Journal of Experimental Medicine (2004) 200 (7): 927–934.
Published: 04 October 2004
Abstract
Most polyreactive and antinuclear antibodies are removed from the human antibody repertoire during B cell development. To elucidate how B cell receptor (BCR) signaling may regulate human B cell tolerance, we tested the specificity of recombinant antibodies from single peripheral B cells isolated from patients suffering from X-linked agammaglobulinemia (XLA). These patients carry mutations in the Bruton's tyrosine kinase ( BTK ) gene that encode an essential BCR signaling component. We find that in the absence of Btk, peripheral B cells show a distinct antibody repertoire consistent with extensive secondary V(D)J recombination. Nevertheless, XLA B cells are enriched in autoreactive clones. Our results demonstrate that Btk is essential in regulating thresholds for human B cell tolerance.
Includes: Supplementary data
Journal Articles
Journal:
Journal of Experimental Medicine
Journal of Experimental Medicine (2004) 200 (2): 191–199.
Published: 19 July 2004
Abstract
Several newly arising human antibodies are polyreactive, but in normal individuals the majority of these potentially autodestructive antibodies are removed from the repertoire by receptor editing or B cell deletion in the bone marrow. To determine what proportion of naturally arising autoantibodies can be silenced by immunoglobulin (Ig) light chain receptor editing, we replaced the light chains in 12 such antibodies with a panel of representative Igκ and Igλ chains. We found that most naturally arising autoantibodies are readily silenced by light chain exchange. Thus, receptor editing may account for most autoreactive antibody silencing in humans. Light chain complementarity determining region (CDR) isoelectric points did not correlate with silencing activity, but Igλ genes were more effective than Igκ genes as silencers. The greater efficacy of Igλ chains as silencer of autoreactivity provides a possible explanation for the expansion and altered configuration of the Igλ locus in evolution.
Includes: Supplementary data
Journal Articles
Journal:
Journal of Experimental Medicine
Journal of Experimental Medicine (2003) 199 (1): 145–150.
Published: 29 December 2003
Abstract
Human B cells that coexpress surrogate and conventional light chains (V-preB + L + ) show an unusual heavy and light chain antibody repertoire that display evidence of receptor editing. However, it is unclear whether V-preB + L + B cells have been silenced by receptor editing or still express autoreactive antibodies. Here we report that 68% of the antibodies expressed by V-preB + L + B cells are autoreactive. A majority of these autoantibodies are true antinuclear antibodies (ANA), and 50% of the ANAs are also reactive with a diverse group of antigens that include dsDNA, ssDNA, immunoglobulin, insulin, and bacterial lipopolysaccharide. Such antibodies are rarely encountered among conventional B cells. We conclude that V-preB + L + B cells are a unique subset of normal circulating human B cells that escape central tolerance mechanisms and express self-reactive antibodies including potentially harmful ANAs.
Includes: Supplementary data
Journal Articles
Journal:
Journal of Experimental Medicine
Journal of Experimental Medicine (2002) 195 (6): 771–780.
Published: 18 March 2002
Abstract
Splenectomized individuals are prone to overwhelming infections with encapsulated bacteria and splenectomy of mice increases susceptibility to streptococcal infections, yet the exact mechanism by which the spleen protects against such infections is unknown. Using congenitally asplenic mice as a model, we show that the spleen is essential for the generation of B-1a cells, a B cell population that cooperates with the innate immune system to control early bacterial and viral growth. Splenectomy of wild-type mice further demonstrated that the spleen is also important for the survival of B-1a cells. Transfer experiments demonstrate that lack of these cells, as opposed to the absence of the spleen per se, is associated with an inability to mount a rapid immune response against streptococcal polysaccharides. Thus, absence of the spleen and the associated increased susceptibility to streptococcal infections is correlated with lack of B-1a B cells. These findings reveal a hitherto unknown role of the spleen in generating and maintaining the B-1a B cell pool.