Blood–brain barrier (BBB) dysfunction has been suggested to play an important role in epilepsy. However, the mechanism mediating the transition from cerebrovascular damage to epilepsy remains unknown. Here, we report that endothelial cyclin-dependent kinase 5 (CDK5) is a central regulator of neuronal excitability. Endothelial-specific Cdk5 knockout led to spontaneous seizures in mice. Knockout mice showed increased endothelial chemokine (C-X-C motif) ligand 1 (Cxcl1) expression, decreased astrocytic glutamate reuptake through the glutamate transporter 1 (GLT1), and increased glutamate synaptic function. Ceftriaxone restored astrocytic GLT1 function and inhibited seizures in endothelial Cdk5-deficient mice, and these effects were also reversed after silencing Cxcl1 in endothelial cells and its receptor chemokine (C-X-C motif) receptor 2 (Cxcr2) in astrocytes, respectively, in the CA1 by AAV transfection. These results reveal a previously unknown link between cerebrovascular factors and epileptogenesis and provide a rationale for targeting endothelial signaling as a potential treatment for epilepsy.
Epilepsy, characterized by recurrent seizures, affects 65–70 million people worldwide (Moshé et al., 2015; Thijs et al., 2019). Although the hyperexcitability underlying epilepsy is believed to be caused by an imbalance of synaptic excitation and inhibition (Li et al., 2011; Neumann et al., 2017; Paz and Huguenard, 2015), antiepileptic strategies directly targeting neuronal excitability have proven to be insufficient in a significant proportion of patients (Löscher and Schmidt, 2006; Eyo et al., 2017; Ferlazzo et al., 2017). This insufficiency points to the need to identify the cause of the imbalance between excitation and inhibition. Drugs targeting the underlying mechanism of this imbalance may prove to be more effective than current antiepileptic medications.
The microvasculature at the blood–brain barrier (BBB) plays an important role in the maintenance of brain homeostasis (Obermeier et al., 2013; Tran et al., 2016). BBB microvascular dysfunction has been suggested to contribute to brain disorders including epilepsy (Blanchette and Daneman, 2015; Obermeier et al., 2013). However, the molecular events linking microvascular pathology to epilepsy remain elusive (Han et al., 2017). Cyclin-dependent kinase 5 (CDK5) is important in several biological processes, including cell proliferation (Pu et al., 2017), sprouting (Tian et al., 2010), and migration (Lampropoulou et al., 2018; Liebl et al., 2010). CDK5 inhibition suppresses angiogenesis in hepatocellular carcinoma (Herzog et al., 2016) and human endothelial cells (ECs; Rárová et al., 2018), and retards the development of endothelial senescence and atherosclerosis (Bai et al., 2012). Endothelial-specific Cdk5 KO in mice also inhibits melanoma tumor growth and improves the sensitivity to anti-angiogenic treatment (Merk et al., 2016).
Until now, research focusing on microvascular function in epilepsy has not been extensive, and experimental microvascular pathology–related epilepsy models are lacking. In this study, we found that endothelial-specific Cdk5 KO in mice induced spontaneous hippocampal epileptic discharges in an age-dependent manner. Our evidence further suggests that endothelial Cdk5 deletion down-regulates astrocytic GLT1-mediated current through endothelial chemokine (C-X-C motif) ligand 1 (CXCL1) and its receptor chemokine receptor 2 (CXCR2)-induced progressive reactive astrogliosis. The reduced GLT1 function increases glutamate synaptic current and, thus, may contribute to the development of epilepsy.
Results and discussion
Endothelial conditional deletion of Cdk5 induces spontaneous seizures
To investigate the role of endothelial Cdk5 in brain, we generated three sets of conditional endothelial-specific Cdk5 KO mice (Tan et al., 2019; Cdh5-Cre;Cdk5f/f mice, Cdh5-CreERT2;Cdk5f/f mice, BR1-iCre-Cdk5f/f mice; Fig. S1, A–E). First, to determine the specificity of Cre recombinase expression in ECs, the Cdh5-Cre or Cdh5-CreERT2 mice line was crossed with the Ai14 reporter mice line (Rosa-CAG-LSL-tdTomato-WPRE::deltaNeo). We found that tdTomato was expressed almost exclusively in Lectin+ (an EC marker) ECs (Fig. S1, F and G), indicating sufficient and specific Cre expression in ECs. Second, brain microvasculature EC (BMVEC)–targeted adeno-associated virus (AAV; termed AAV-BR1) was further used to delete target molecules solely in brain ECs (Körbelin et al., 2016). Consistent with previous reports, robust enhanced GFP (EGFP) expression was largely restricted to the brain vasculature in AAV-BR1-EGFP–injected mice (Fig. S1 H). Moreover, the KO had no effect on body weight, the organ index (Fig. S1 I), or brain vascular morphology (Fig. S1 J).
Cdh5-Cre;Cdk5f/f mice showed an age-dependent increase in the prevalence and frequency of seizures using 24-h video surveillance (Fig. 1 A). Epileptic seizures were also confirmed by local field potential recordings in the hippocampus in 11 of 17 16-wk-old Cdh5-Cre;Cdk5f/f mice (Fig. 1 B and Video 1). No significant electroencephalographic (EEG) changes were observed in the cortex (Fig. 1 B). Spontaneous seizures were not observed in control littermates. Furthermore, epileptic waves were also recorded in 6 of 10 8-wk-old Cdh5-CreERT2;Cdk5f/f mice (treated with tamoxifen at 4 wk; Fig. 1 C) and in 6 of 12 8-wk-old Cdk5f/f mice (i.v. injection with BR1-iCre virus at 4 wk; Fig. 1 D) in the hippocampus. No spontaneous seizures were observed in 4-wk-old Cdh5-Cre;Cdk5f/f mice. For mice with no spontaneous seizures, their sensitivity to the convulsant drug pentylenetetrazol (PTZ) was measured. Interestingly, our data showed a decrease in onset time (95.40 ± 8.20 s vs. 171.00 ± 32.83 s) and an increase in duration of seizures induced by PTZ (110.50 ± 18.74 s vs. 55.00 ± 11.75 s; Fig. 1 E) compared with control (Cdk5f/f) mice. Similar results were observed in Cdh5-CreERT2;Cdk5f/f mice (Fig. S1 K) and BR1-iCre–injected Cdk5f/f mice (Fig. S1 L). These results suggest that endothelial Cdk5 deficit leads to the development of spontaneous epilepsy.
The increase in hippocampal discharges may have been caused by an imbalance between excitatory and inhibitory neurons (Lopez-Santiago et al., 2017). Using microdialysis in freely moving mice, we found a significant increase in extracellular glutamate in the hippocampus in Cdh5-Cre;Cdk5f/f mice (Fig. 1 F). Whole-cell recordings in brain slices showed an increased excitability of hippocampal CA1 pyramidal neurons in Cdh5-Cre;Cdk5f/f mice. There were no significant differences in intrinsic membrane properties of pyramidal neurons between the groups at 4 wk (Fig. S1 M). This increase in excitability was blocked by the glutamate receptor antagonist DL-2-amino-5-phosphonovaleric acid (DL-AP5) and 6-cyano-7-nitroquinoxaline-2,3-dione (Fig. 1, G and H). In contrast, no significant changes in excitability were observed in medial prefrontal cortex pyramidal neurons (Fig. S1 N). Hippocampal CA1 pyramidal neurons also showed an increased response to exogenously applied glutamate (100 µM; Fig. 1 I), and an increase in both the amplitudes and frequencies of spontaneous and miniature excitatory postsynaptic current (sEPSC and mEPSC, respectively; Fig. 1, J–N). Endothelial Cdk5 KO, however, had no effect on spontaneous and miniature inhibitory postsynaptic current (sIPSC and mIPSC, respectively; Fig. S1, O–Q). The lack of changes in the paired pulse ratio (PPR) of evoked EPSC (Fig. S1 R) indicated that the increases in the mEPSC and sEPSC frequencies were not attributed to increases in presynaptic release probability. There were also no changes in the morphology or density of dendritic spines in CA1 pyramidal neurons between 4-wk-old mutant and control mice (Fig. S1, S and T). Similarly, the increased amplitudes and frequencies of sEPSC/mEPSC were also observed in Cdh5-CreERT2;Cdk5f/f mice (treated with tamoxifen at 4 wk; Fig. S1, U–W) and Cdk5f/f mice (i.v. injection with BR1-iCre virus at 4 wk; Fig. S1, X–Z).
These data suggested that selective deletion of Cdk5 in ECs increases CA1 neuronal hyperexcitability and seizure generation. More importantly, the increases in both the amplitudes and frequencies of sEPSC and mEPSC suggested that synaptic levels of glutamate were higher in Cdh5-Cre;Cdk5f/f mice than in Cdk5f/f mice. However, no significant PPR changes or normal spine morphology were observed in Cdh5-Cre;Cdk5f/f mice. Therefore, we propose that abnormal extracellular excitatory neurotransmitter levels trigger hyperexcitability of pyramidal neurons of the hippocampus in endothelial Cdk5-deficient mice at 4 wk.
Deletion of endothelial Cdk5 induces progressive astrogliosis and impairs astroglial GLT1 function
The above results demonstrated that the hyperexcitability of hippocampal neurons induced by endothelial Cdk5 deficiency resulted from increased extracellular glutamate levels. Since astrocytes can take up glutamate and play an important role in glutamate homeostasis (Takahashi et al., 2015), we measured glial fibrillary acidic protein (GFAP) levels and found them to be significantly higher in the hippocampus but not in the cortex in endothelial Cdk5 KO mice (Fig. 2, A and B). Immunostaining further showed a significant increase in the number of GFAP+ astrocytes in mutant mice compared with control mice (Fig. 2, C and D). Furthermore, the number of cells expressing both GFAP and S100β, an astrocytic marker associated with astrocyte proliferation and distress (Tynan et al., 2013), was also significantly increased in the hippocampus of 4-wk-old Cdh5-Cre;Cdk5f/f mice compared with Cdk5f/f mice (Fig. 2, E and F). However, no astrogliosis was observed in the cortex (Fig. S2 A). These results suggested that the hippocampus was more sensitive than the cortex to endothelial Cdk5 deficiency.
Whole-cell recordings from hippocampal CA1 astrocytes showed significantly higher input resistance (Rin: 20.29 ± 2.05 mΩ vs. 15.73 ± 1.03 mΩ), membrane time constants (τm: 1.12 ± 0.21 ms vs. 0.70 ± 0.06 ms), and membrane capacitance (Cm: 60.49 ± 8.59 pF vs. 43.26 ± 1.55 pF; Fig. 2 G), and no significant changes in resting membrane potential (RMP) in endothelial Cdk5-deficient astrocytes compared with control astrocytes of Cdk5f/f mice at 4 wk (−80.41 ± 0.71 mV vs. −80.38 ± 0.65 mV; Fig. 2 G). In Cdh5-Cre;Cdk5f/f mice, the increased Cm is consistent with the phenotype of astrogliosis as described previously (Hirrlinger et al., 2010; Iandiev et al., 2006).
To further determine whether endothelial Cdk5 deficiency reduces astrocytic glutamate transport, N-methyl-D-aspartate, α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid, and γ-aminobutyric acid type A receptors, as well as voltage-gated Na+ and Kir4.1 channels, were pharmacologically blocked (Fig. 2, H and I). Application of 100 µM glutamate induced an inward current, part of which was blocked by DL-threo-β-benzyloxyaspartic acid (TBOA), an inhibitor for glutamate/aspartate transporter (GLAST) and GLT1 glutamate transporters. In Cdh5-Cre;Cdk5f/f astrocytes, the TBOA-sensitive current was significantly reduced compared with Cdk5f/f astrocytes (122.20 ± 4.77 pA vs. 192.30 ± 7.30 pA; Fig. 2 H). Experiments with dihydrokainic acid (DHK), which blocked only GLT1 transporters, further showed that the difference in TBOA-sensitive current between the two groups was largely attributed to a decrease in the GLT1-mediated current (83.34 ± 1.34 pA [Cdh5-Cre;Cdk5f/f] vs. 148.70 ± 1.66 pA [Cdk5f/f]) but not the GLAST-mediated current (37.83 ± 4.77 pA [Cdh5-Cre;Cdk5f/f] vs. 43.60 ± 7.30 pA [Cdk5f/f]; Fig. 2 I). A decrease in GLT1-mediated current was also observed in Cdh5-CreERT2;Cdk5f/f mice (84.89 ± 4.88 pA [Cdh5-CreERT2;Cdk5f/f] vs. 153.40 ± 5.63 pA [Cdk5f/f]; Fig. S2 B) and BR1-iCre virus-injected Cdk5f/f mice (82.54 ± 3.06 pA [BR1-iCre-injected-Cdk5f/f] vs. 155.30 ± 4.63 pA [BR1-Con-injected-Cdk5f/f]; Fig. S2 C). Therefore, astrogliosis triggered by endothelial Cdk5 deficiency weakened astrocytic GLT1-mediated current.
The β-lactam antibiotic ceftriaxone (CEF) is a potent GLT1 translational activator (Higashimori et al., 2016). Treatment with CEF increased GLT1 expression in the hippocampus 2.6-fold in Cdh5-Cre;Cdk5f/f mice and 2.3-fold in control mice. It had no effects on the expression of GLAST (Fig. 2, J and K), the astrocytic protein glutamine synthetase, or the neuronal glutamate transporter EAAC1 (Fig. S2, D and E). CEF delayed the onset (191.80 ± 34.02 s [CEF] vs. 89.33 ± 4.67 s [saline]) and shortened the duration (34.25 ± 10.86 s [CEF] vs. 106.00 ± 17.06 s [saline]) of PTZ-induced convulsions in 4-wk-old Cdh5-Cre;Cdk5f/f mice (Fig. 2 L). It also reversed the hyperexcitability of CA1 pyramidal neurons (Fig. 2, M and N) without affecting the intrinsic membrane properties of these neurons (Fig. S2 F). CEF increased the GLT1-mediated current in astrocytes, and the increase seen in Cdh5-Cre;Cdk5f/f mice (172.60 ± 1.99 pA vs. 87.06 ± 2.34 pA; Fig. 2, O and P) was significantly higher than that in Cdk5f/f mice (170.90 ± 2.14 pA vs. 156.40 ± 2.66 pA). CEF also decreased extracellular glutamate in both mutant and control mice (Fig. S2 G).
To further test whether restoration of GLT1 function reversed the effects of endothelial Cdk5 deficiency, we injected an AAV expressing GFAP promoter-driven Cre-dependent GLT1-mCherry into the CA1 of Cdk5f/f and Cdh5-Cre;Cdk5f/f mice (Fig. S2, H–K). The treatment significantly delayed the onset of seizures (196.30 ± 19.10 s vs. 83.00 ± 16.26 s) and decreased seizure duration induced by PTZ (39.00 ± 6.75 s vs. 132.30 ± 13.91 s) in Cdh5-Cre;Cdk5f/f mice infected with AAV-GLT1 virus compared with AAV-mCherry infection (Fig. S2 L). AAV-GLT1 injection also reversed the increase in amplitudes and frequencies of sEPSC/mEPSC (Fig. S2, M–O) and the decrease in GLT1-mediated current (173.00 ± 5.58 pA vs. 86.49 ± 3.44 pA) in Cdh5-Cre;Cdk5f/f mice (Fig. S2 P).
Taken together, the above results suggested that the phenotypes observed in Cdh5-Cre;Cdk5f/f mice resulted, at least partially, from a decrease in GLT1 function in hippocampal astrocytes and that they can be reversed by increasing GLT1 expression.
Cdk5 deficiency induces overexpression of EC-derived CXCL1
To investigate the role of the BBB in the above-described effects induced by endothelium-specific Cdk5 KO, we analyzed BBB permeability and the expression of tight-junction proteins. BBB leakage, evidenced by Evans blue extravasation and positive immunostaining for astrogliosis, was present in 16-wk-old, but not 4-wk-old, Cdh5-Cre;Cdk5f/f mice (Fig. 3 A). Examination of exogenous tracer effusion (Fig. 3 B) and investigation of perivascular deposits of the plasma-derived proteins fibrinogen and IgG yielded similar results (Fig. 3 C). BBB disruption in 16-wk-old mutant mice was further confirmed by transmission electron microscopy (Fig. 3 D), immunoblotting for Claudin-5 (Fig. 3 E), and immunostaining for ZO-1 (Fig. 3 F). BBB damage is unlikely to be responsible for the decreased threshold for PTZ-induced seizures seen in 4-wk-old mice since it was not observed at that age.
Next, to identify the molecular mechanism that links endothelial Cdk5 deficiency to astrogliosis, we cultured primary ECs from the cerebral microvessels of 4-wk-old Cdh5-Cre;Cdk5f/f and Cdk5f/f mice (Tan et al., 2019). Using gene expression microarrays combined with network analysis, we found that the transcripts that existed in primary ECs of both groups could be classified into two clusters (Fig. 3 G). Of the 20,210 robustly expressed genes, 986 expressed differentially between the mutant and control mice (Fig. S3 A). Most of these genes were up-regulated in the Cdh5-Cre;Cdk5f/f mice when compared with the Cdk5f/f mice. Expression analysis of Kyoto Encyclopedia of Genes and Genomes pathway analysis (Fig. S3 B) combined with Gene Ontology annotation (Fig. S3 C) and regulatory genes upstream of the differentially expressed genes (Fig. S3. D) showed that genes involved in IFNG, IL1B, TNF, and cell adhesion molecule events were related to endothelial activation (Berberich et al., 2011; Wu et al., 2015). Consistent with this activation pattern, several members of the Cxcl chemokine gene family were identified as differentially expressed, including Cxcl1, the level of which was found to be significantly increased in Cdh5-Cre;Cdk5f/f mice. No changes were found in the Cxcl16 level (Fig. 3 H).
Furthermore, these findings were confirmed by quantitative RT-PCR (qRT-PCR; Fig. 3 I) and ELISA assay (Fig. 3 J). The mRNA levels of Cxcl2, Cxcl9, Cxcl10, and Cxcl11 (Fig. S3 E) were unchanged in Cdh5-Cre;Cdk5f/f mice. In situ hybridization further showed that Cxcl1 mRNAs existed in ECs by using RNAscope (Fig. 3 K), suggesting its presence in ECs.
Overall, these data will be helpful for filling the current gaps in our knowledge, including those regarding the mechanisms of EC-induced aberrations in astrocytic function that eventually led to the hyperexcitability of pyramidal neurons that occurred during the pathological processes of spontaneous recurrent seizures.
Aberrant elevation of EC-derived CXCL1 is the trigger of astrogliosis
To provide in vitro evidence showing that endothelial CXCL1 is necessary for the effects of Cdk5 on astrocytes, we cultured hippocampal astrocytes from 3-wk-old Cdk5f/f mice. Application of recombinant CXCL1 protein (20 ng for 6 h) directly to cultured hippocampal astrocytes, which mimicked the effects of endothelial Cdk5 KO, increased the number of cells expressing the astrocytic markers GFAP and S100β (Fig. 4 A), decreased the GLT1-mediated current (83.31 ± 3.00 pA vs. 126.90 ± 4.91 pA), and had no significant effects on the GLAST-mediated current (59.30 ± 3.51 pA vs. 58.75 ± 4.77 pA) or GLT1 expression (Fig. 4 B and Fig. S3, F and G).
To further test whether CXCL1 expression is a required step through which endothelial Cdk5 KO induces astrogliosis and suppresses GLT1 function, we used shRNA to silence Cxcl1 expression in ECs in vivo (Fig. 4, C and D). Administration of AAV-BR1-shCxcl1, but not its control (AAV-BR1-Con), eradicated the astrogliosis in the Cdh5-Cre;Cdk5f/f mice (Fig. 4 E), delayed the onset (208.70 ± 46.20 s vs. 61.75 ± 11.80 s), and shortened the duration of PTZ-induced seizures (30.67 ± 6.89 s vs. 107.5± 15.18 s; Fig. 4 F). Cxcl1 silencing also reversed the increase caused by Cdk5 deletion in the amplitudes and frequencies of sEPSC/mEPSC in CA1 pyramidal neurons (Fig. 4, G–K) and restored the GLT1-mediated current (Cdk5f/f: 181.80 ± 4.37 pA vs. 156.30 ± 6.81 pA; Cdh5-Cre;Cdk5f/f: 181.30 ± 4.71 pA vs. 87.48 ± 5.28 pA; Fig. 4 L).
We also studied the effect of a CXCL1-neutralizing antibody both in vivo and in vitro. The antibody delayed the onset (229.00 ± 44.77 s vs. 72.60 ± 16.02 s) and reduced the duration of PTZ-induced seizures (51.00 ± 18.50 s vs. 123.20 ± 16.44 s) in Cdh5-Cre;Cdk5f/f mice (Fig. S3 H). The treatment with the antibody also reversed the increase in excitability and in amplitudes and frequencies of sEPSC/mEPSC in CA1 pyramidal neurons (Fig. S3, I–L). It is unlikely that the CXCL1-neutralizing antibody acted directly on pyramidal neurons since the treatment had no effect on primary cultured hippocampal neurons (Fig. S3 M). It also had no effect on the intrinsic membrane properties of pyramidal neurons obtained from mutant or control mice (Fig. S3 N). CXCL1 neutralization also restored the GLT1-mediated current and eradicated the difference in Cdh5-Cre;Cdk5f/f mice (166.40 ± 4.22 pA vs. 85.32 ± 2.86 pA; Fig. S3 O).
These results provide further evidence for a role of endothelial CXCL1 in the phenotypes seen in endothelial Cdk5 KO mice. These findings may provide insight into the interactions between ECs and astrocytes associated with synaptic homeostasis and/or pathological mechanisms of neurological disorders.
Endothelial CXCL1 regulates astrocytic glutamate uptake via astroglial CXCR2 receptor
The biological effects of chemokines are mediated by G-protein-coupled chemokine receptors. CXCR2 is the primary receptor for CXCL1 (Brandenburg et al., 2016; Cao et al., 2016; Cao and Malon, 2018; Horuk et al., 1997; Liu et al., 2014). The interaction of CXCL1 with CXCR2 plays an important role in inflammation (Miyake et al., 2013). We confirmed its presence in both astrocytes and neurons (Fig. 5 A and Fig. S3 P). To determine which CXCR2 receptors are responsible for CXCL1’s effects, we used AAV2/9-GFAP-Cxcr2-RNAi and AAV2/9-CaMKIIα-Cxcr2-RNAi to silence Cxcr2 in astrocytes and pyramidal neurons, respectively (Fig. 5, B–D; and Fig. S3, Q–S).
Silencing of astrocytic Cxcr2 in Cdh5-Cre;Cdk5f/f mice resulted in longer latency (233.30 ± 32.66 s vs. 61.50 ± 9.19 s) and reduced the duration of PTZ-induced seizures (37.00 ± 14.62 s vs. 109.50 ± 5.81 s; Fig. 5 E). However, Cxcr2-specific silencing in pyramidal neurons had no effect on PTZ-induced seizures in Cdh5-Cre;Cdk5f/f mice (onset: 62.67 ± 6.06 s vs. 66.00 ± 5.13 s; duration: 112.30 ± 8.37 s vs. 120.30 ± 22.45 s; Fig. S3 T). Consistently, the differences in the amplitudes and frequencies of sEPSC and mEPSC between AAV-Con–injected Cdk5f/f mice and Cdh5-Cre;Cdk5f/f mice were eliminated in Cxcr2-RNAi–transducted astrocytes (Fig. 5, F–K), but not in Cxcr2-RNAi–transducted pyramidal neurons (Fig. S3, U–W). As shown in Fig. 5 L, Cxcr2-RNAi transduction restored the GLT1 current in the astrocytes (180.60 ± 5.97 pA vs. 87.24 ± 3.31 pA), whereas Cxcr2 knockdown in pyramidal neurons had no effect on GLT1 current in Cdh5-Cre;Cdk5f/f mice (88.49 ± 6.38 pA vs. 88.12 ± 2.97 pA; Fig. S3 X). These results support a working model in which CXCL1 reduces GLT1-mediated glutamate uptake by activation of CXCR2 receptors on astrocytes.
In summary, our findings reveal a previously unknown function of the endothelial-derived Cdk5 signaling in the brain. Endothelial Cdk5 deficiency induces spontaneous epilepsy in an age-dependent manner. The effect is associated with a decrease in GLT1-mediated glutamate uptake and an increase in excitability of hippocampal pyramidal neurons. Our evidence further suggests that these effects depend on CXCL1 release from ECs and subsequent activation of astrocytic CXCR2 receptors by CXCL1. Importantly, we found that these effects can be reversed by pharmacological restoration of GLT1 function (CEF), genetic silencing or immunoneutralization of CXCL1, or inhibition of the CXCL1 receptor CXCR2 on astrocytes. These findings warrant further investigation of endothelial Cdk5 and its downstream pathways as potential new targets for the treatment of epilepsy.
Materials and methods
Mice and genotyping
Mice were housed under a 12-h light/dark cycle at a constant temperature of 22 ± 1°C with 40–60% humidity and provided access to standard food and water. The animals acclimated to their environment for ≥1 wk before the initiation of the experimental protocols. All experiments in animals were approved by the Committee for Animal Experiments at Nanjing Medical University and Zhejiang University in China.
Several mouse lines were used for our experiments. Cdh5-Cre mice (Cre expressed under the control of cadherin 5, also known as VEC-cre, stock no. 006137; The Jackson Laboratory) were crossed with mice carrying a loxP-flanked Cdk5 gene (stock no. 014156; The Jackson Laboratory) to generate Cdh5-Cre;Cdk5f/f mice. Ai14 mice (Rosa26-tdTomato Cre reporter line, stock no. 007914; The Jackson Laboratory) and endothelial tamoxifen-inducible driver Cdh5-CreERT2 mice (obtained from Prof. Ralf Adams, Max Planck Institute, Göttingen, Germany) were also used. To induce Cre activity at 4 wk, tamoxifen (10 mg/ml in ethanol/peanut oil; Sigma-Aldrich) was given in three consecutive i.p. injections (0.1 mg/g body weight) at postnatal day 28, 29, and 30. The numbers and ages of the mice used are indicated in the figure legends.
Video/EEG recording of spontaneous seizures
Spontaneous seizure activity was monitored by EEG recording as described previously (Zhou et al., 2019). Mice were anesthetized and mounted in a stereotactic apparatus. For hippocampal recordings, bipolar twisted stainless steel electrodes (0.2 mm in diameter; Plastics One) were placed bilaterally in hippocampus CA1 (anteroposterior, −2.0 mm; mediolateral, ±1.5 mm; dorsoventral, −1.5 mm). Stainless steel screws (MX-0090-2; Plastics One) were placed epidurally and bilaterally over the frontal cortices, 0.5 mm posterior to the bregma and 2.45 mm lateral to the midline. An additional screw was placed just to the right of the frontal sinus and served as a reference electrode. The electrodes were then connected to a plastic pedestal (6 Channel; Plastics One), and the entire assembly was secured with dental cement. After 7 d of recovery from surgery, EEG recording was conducted continuously in freely moving mice with a Vanguard system (Lamont) at a sampling rate of 1 with a high-frequency filter of 70 Hz in synchronization with video recording for 24 h/d. Epileptic seizures were defined as field potentials twofold greater than the basal potential with durations longer than 10 s. Only mice with correctly located electrodes were included in the analysis.
Gelatin and FITC were diluted to a concentration of 1 mg/ml in sterile PBS as previously described (Underly et al., 2017). Mice were anesthetized and perfused with PBS and 4% paraformaldehyde (PFA; 40°C, 50 ml/animal) followed by gelatin-FITC (30°C, 30 ml/animal). After 2 h of incubation in ice, the heads were fixed in 4% PFA overnight. The next day, the brains were removed, fixed in 4% PFA for another 24 h, embedded in PBS, and cut into 35-µm sections on a vibratome. For immunofluorescent labeling, the sections were washed in PBS and incubated with DAPI for ∼20 min. After washing in PBS, the stained sections were examined with a confocal laser-scanning microscope as described previously (Jiang et al., 2017).
PTZ-induced seizure test
4-wk-old mice were i.p. injected with PTZ in 0.9% saline at a dose of 60 mg/kg (injection volume 1 ml/100 g body weight) and individually placed in an acrylic box as previously described (Shen et al., 2016). Seizure behaviors were analyzed for 30 min after PTZ injection. The time to onset and the duration of tonic-clonic seizures were recorded. Behavior experiments were performed during the day between 09:00 and 12:00.
Primary BMVECs were isolated and cultured from 4-wk-old mice following a previous description (Rosas-Hernandez et al., 2018). Briefly, cortex tissue was dissected, then digested with gentle trituration every 10 min for 30 min at 37°C with DMEM containing 10 mM Hepes, 5 mM Ca2+, 10 mg/ml DNase I, and 400 U/ml collagenase. After adequate centrifugation at 1,000 g for 5 min, cell pellets were resuspended in 20% BSA and centrifuged at 1,000 g at 4°C for 20 min. Then, cell pellets were resuspended in EC culture medium (with puromycin) and seeded into plates coated with fibronectin. 2 d later, culture medium was altered with EC culture medium. For 10–12-d culture, cells were collected for further detections.
BBB permeability assays
To assess BBB permeability, fluorescence tracers with different molecular size from small to large, including biocytin-tetramethylrhodamine (TMR; mol wt = 869 daltons; T12921; Thermo Fisher Scientific), Evans blue (mol wt = 960 daltons; E2129; Sigma-Aldrich), or TMR-dextran (mol wt = 40 kD; D1868; Invitrogen), were i.v. injected via the tail vein, respectively (Shi et al., 2016; Tan et al., 2019). 12 h later, the mice were anesthetized and transcardially perfused with 0.01 mol/liter PBS, followed by 4% PFA. The brains were collected and dehydrated in 30% sucrose in PBS, and frozen serial coronal brain sections (40 µm thick) were performed on a cryostat (Leica CM1900). Sections were processed for direct fluorescent detection of Alexa594. Images were acquired using a confocal fluorescence microscope (Zeiss LSM800). The regions of interest from the hippocampus were scanned at a resolution of 1,024 × 1,024 pixels with a 40× objective lens.
Immunocytochemistry was performed as previously described (Wang et al., 2015). In brief, mice were anesthetized and transcardially perfusion fixed with 4% PFA in PBS. Horizontal brain slices (40 µm thick) were prepared by using a vibratome (Leica VT 1000 S), and sections were incubated for 30 min at room temperature with 0.1% Triton X-100 in PBS and for another 1 h in 3% BSA in PBS. For immunolabeling, the sections were incubated with indicated primary antibodies (anti-glucose transporter 1 [Glut1; 1:300, ab40084; Abcam], anti-S100β [1:500, ab52642; Abcam], anti-GFAP [1:500, MAB360; Millipore], anti-CDK5 [1:300, 2506; Cell Signaling Technology], anti-CaMKII [1:300, ab22609; Abcam], anti-ZO-1 [1:250, 402200; Invitrogen], anti-fibrinogen [1:200, ab34269; Abcam], anti-IgG [1:200, A-21203; Invitrogen], anti-Iba 1 [1:200, ab5076; Abcam], anti-NeuN [1:500, ABN78; Millipore], anti-Lectin [1:300, FL-1171; Vector], and anti-CXCR2 [1:200, ab14935; Abcam]) overnight at 4°C in the dark. Then, the sections were incubated with Alexa Fluor 488–conjugated anti-mouse IgG (1:300, A21202; Life Technologies), Alexa Fluor 594–conjugated anti-rabbit IgG (1:300, A21207; Life Technologies), and Alexa Fluor 488–conjugated anti-goat IgG (1:300, A11055; Life Technologies) for 1 h at 25°C. Images were acquired using a Zeiss LSM 800 confocal microscope.
Western blot analysis
Western blot analysis was performed according to protocols as previously described (Lu et al., 2014). In brief, hippocampus tissues were separated and homogenized in lysis buffer. The equivalent amount of protein was subjected to SDS-PAGE gel (10–12%) and transferred to PVDF membranes (Millipore). The blots were probed with anti-GFAP (1:3,000, MAB360; Millipore), anti-GLAST (1:5,000, ab416; Abcam), anti-GLT1 (1:5,000, ab41621; Abcam), anti-EAAC1 (1:10,000, ab124802; Abcam), anti-glutamine synthetase (1:3,000, ab73593; Abcam), anti-Claudin-5 (1:2,000, 35–2500; Invitrogen), anti-GAPDH (1:5,000, 2118; Cell Signaling Technology,) and anti-β-actin (1:10,000, A1978; Sigma-Aldrich) at 4°C overnight, and then incubated with HRP-conjugated secondary antibodies. The proteins were visualized by an enhanced chemiluminescence detection system (Amersham Life Science). The density of protein bands was quantified using ImageJ software (US National Institutes of Health) and normalized to actin or GAPDH.
Hippocampal and cortical slices were prepared with a vibratome (Leica VT 1000 S) in ice-cold cutting artificial cerebrospinal fluid (ACSF) containing (in mM) 125 NaCl, 3 KCl, 1.25 NaH2PO4, 2 MgSO4, 2 CaCl2, 25 NaHCO3, and 10 glucose, saturated with 95% O2 and 5% CO2, as previously described (Tan et al., 2019). After recovery for 30 min at 34°C in oxygenated ACSF, the slices were incubated at room temperature for ∼60 min. To measure the effect of the CXCL1-neutralizing antibody on electrophysiology recordings, the slices were incubated with 100 ng/ml CXCL1-neutralizing antibody (MAB4532; R&D Systems) in oxygenated ACSF for ∼2 h before the recording series.
Pyramidal neurons and astrocytes were visualized with an infrared-sensitive charge-coupled device camera with a 40× water-immersion lens (Olympus), and whole-cell patch-clamp recordings were performed (MultiClamp 700B Amplifier, Digidata 1440A analogue-to-digital converter). The patch-clamp intracellular solution used for pyramidal neurons contained (in mM) 130 potassium, 20 KCl, 10 Hepes, 4 Mg-ATP, 0.3 Na-GTP, 10 disodium phosphocreatine, and 0.2 EGTA (pH 7.25, adjusted with KOH; 288 mOsm). Tight seals were established using glass micropipettes with 3–8-MΩ open-pipette resistance at −70 mV for pyramidal neurons and at −80 mV for astrocytes. For current-clamp recording, action potentials (APs) were recorded under 750-ms suprathreshold current of 0–300 pA in 10-pA steps.
The passive membrane properties were recorded after obtaining the whole-cell configuration. Rin was induced with a rectangular hyperpolarizing current of −60 to 10 pA in 10-pA steps. τm was fit by an exponential function of the membrane potential change in response to rectangular hyperpolarizing current injection that induced small (3–5 mV) voltage deflections. Cm was obtained by dividing τm by Rin.
To isolate sEPSC, 50 µM picrotoxin (PTX; Tocris Bioscience), a γ-aminobutyric acid type A receptor blocker, was added to the ACSF. mEPSCs were recorded in the presence of 50 µM PTX and 1 µM tetrodotoxin (TTX; Tocris Bioscience), which blocks sodium current. To isolate sIPSC, the bath solution containing 50 µM DL-AP5 (Tocris Bioscience; to block N-methyl-D-aspartate receptors) and 20 µM CNQX (Tocris Bioscience; to block α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptors) was used. mIPSCs were recorded in the presence of 50 mM DL-AP5, 20 µM CNQX, and 1 µM TTX. The holding potential for EPSC recordings was −70 mV. Recordings were accepted under the condition that resistance was <20 MΩ.
Glutamate transport current was recorded according to the methods in previous reports (Armbruster et al., 2016; Armbruster et al., 2014; Diamond, 2005; Hanson et al., 2015). Briefly, for astrocytes, the recording pipettes were filled with a solution containing (in mM) 140 KCl, 0.5 CaCl2, 1 MgCl2, 5 EGTA, 10 Hepes, 3 Mg-ATP, and 0.3 Na-GTP (pH 7.2–7.3, adjusted with KOH; 288 mOsm). Astrocytes were identified by the following electrophysiological properties (Ge et al., 2006): an RMP of −80 mV and smaller Rin, larger capacitance, and a more linear I-V curve than other types of glial cells. The identified astrocytes were used for glutamate uptake–mediated current analyses. In voltage-clamp mode, whole-cell patch-clamped astrocytes were held at −80 mV. To record the transport current, the following drugs were added to the bath solution: 50 µM DL-AP5, 20 µM CNQX, 50 µM PTX, 1 µM TTX, and 0.1 mM BaCl2 (Sigma-Aldrich). Then, 100 µM glutamate was applied by puff application for 500 ms to activate transport current. TBOA (0.3 mM; Tocris Bioscience) was used to isolate the total (GLT1+GLAST) glutamate transport current, and 100 µM DHK (Tocris Bioscience) was used to isolate the GLT1-mediated current. All analyzed and presented transport currents are TBOA or DHK subtractions. Data acquisition and analysis were performed with pClamp 10.3 software.
In vivo microdialysis experiments
A unilateral guide cannula was implanted into hippocampal CA1 regions (anteroposterior, −2.0 mm; mediolateral, ±1.5 mm; dorsoventral, −1.5 mm) of Cdk5f/f and Cdh5-Cre;Cdk5f/f mice as described previously (Berger et al., 2018). After 7 d of recovery, a microdialysis probe (AZ-2-01) was inserted and secured to the guide cannula for dialysate collection. After 90 min of ACSF circulation at a rate of 1 µl/min, dialysis samples were gathered every 20 min for three times at a rate of 0.5 µl/min with a microinfusion pump (ESP-32), and the glutamate concentrations were analyzed using an HPLC system.
CEF and vehicle treatment
Mice were i.p. injected with a 200-mg/kg body weight dose of CEF (Rocephin; Roche) dissolved in 0.9% saline from 09:00 to 10:00 for 5 consecutive d as described previously (Hefendehl et al., 2016). Control groups of Cdh5-Cre;Cdk5f/f and Cdk5f/f mice were given equivalent injections of 0.9% saline for 5 consecutive d.
Drug microinjection by guide cannula
Bilateral hippocampus cannulas were implanted in targeted coordinates of hippocampus CA1 (anteroposterior: −2.0 mm; mediolateral: ±1.5 mm; dorsoventral: −1.5 mm) in 4-wk-old mice (John et al., 2012). After 7-d recovery from surgery, a CXCL1 neutralizing antibody (1 µg/2 µl) was administered via the guide cannula using a micro syringe pump at a rate of 46 nl/s according to instructions.
Transmission electron microscopy
Transmission electron microscopy was performed as previously described (Wang et al., 2017a). Mice were anesthetized and transcardially perfused with 4% PFA in PBS. The brain was removed and fixed with 4% PFA at 4°C overnight. Horizontal brain slices (200 µm thick) were prepared using a vibratome (Leica VT 1000 S). The target hippocampal tissues were cut into 0.5 × 0.5-cm squares, fixed with 2.5% glutaraldehyde at room temperature, and incubated to 4°C overnight. The sections were postfixed in 1% osmium tetroxide for 1 h; gradient-dehydrated in 50%, 70%, 90%, and 100% ethanol; and embedded in epoxy resin. Polymerization was confirmed before the blocks were cut into ultrathin sections with an ultramicrotome. The sections were viewed under an electron microscope (Hitachi 7000; Nikon) after being stained with uranyl acetate and lead citrate.
High-throughput mRNA sequencing
Primary cultured EC samples from Cdk5f/f and Cdh5-Cre;Cdk5f/f mice were collected for total RNA extracts using TRIzol Reagent (Invitrogen) followed by quality check on an Agilent 2100 Bioanalyzer (Agilent Technologies), a NanoDrop spectrophotometer (Thermo Fisher Scientific), and a 1% agarose gel. Qualified samples, which had an RNA integrity number >7, were retained for next-generation library preparation according to the manufacturer’s protocol (an NEBNext Ultra RNA Library Prep Kit for Illumina). RT and cDNA modification were performed as described previously (Best et al., 2019). After that, DNA sequencing of the libraries was performed on an Illumina sequencer, and data were analyzed by GENEWIZ.
RNA isolation and real-time qPCR
Mouse brain ECs were isolated as described above (Navone et al., 2013). The samples were homogenized, and total RNA was isolated using RNAiso Plus (Takara). cDNA was synthesized using a PrimeScript RT Reagent Kit with gDNA Eraser (Takara) in accordance with the manufacturer’s instructions. qRT-PCR was performed with EvaGreen 2× qPCR MasterMix-No Dye (abm) to detect the mRNA expression level of Cdk5, Cxcl1, Cxcl2, Cxcl9, Cxcl10, Cxcl11, Cxcl16, and Gapdh (as an internal control). The details of primer sequences are as follows: Cdk5 (forward), 5′-CAATGCAGAAATACGAGAAACTGG-3′; Cdk5 (reverse), 5′-CTTTGAGTAGACAGATCTCCCG-3′; Cxcl1 (forward), 5′-ACCGAAGTCATAGCCACACTC-3′; Cxcl1 (reverse), 5′-CTCCGTTACTTGGGGACACC-3′; Cxcl2 (forward), 5′-GGCCACCAACCACCAGGCTA-3′; Cxcl2 (reverse), 5′-TTCCGTTGAGGGACAGCAGCC-3′; Cxcl9 (forward), 5′-GCTCTGCCATGAAGTCCGCTGT-3′; Cxcl9 (reverse), 5′-GCAATTGGGGCTTGGGGCAA-3′; Cxcl10 (forward), 5′-AGCGCTTCATCCACCGCTGA-3′; Cxcl10 (reverse), 5′-GGGCAGGATAGGCTCGCAGG-3′; Cxcl11 (forward), 5′-CCCGAGTAACGGCTGCGACA-3′; Cxcl11 (reverse), 5′-GGGCTCACAGTCAGACGTTCCC-3′; Cxcl16 (forward), 5′-TGGCACCCAGATACCGCAGG-3′; Cxcl16 (reverse), 5′-ATGTGCAGGGGTGCTCGTGT-3′.
The CXCL1 protein levels of primary cultured ECs extracted from Cdk5f/f and Cdh5-Cre;Cdk5f/f mice were detected with mouse CXCL1/KC Quantikine ELISA Kit (R&D Systems; Wang et al., 2017b; Taki et al., 2018). In brief, samples were incubated for 2 h and washed with Quantikine wash buffer. The absorbance was measured at 450 nm with a 540-nm correction, and the concentrations were calculated according to the manufacturer’s protocols.
RNAscope in situ hybridization assay
RNA in situ hybridization assay was performed to detect the expression of endothelial Cxcl1 mRNA transcripts using an RNAscope Multiplex Fluorescent Reagent Kit v2 (Advanced Cell Diagnostics; Pacher et al., 2018; Valenta et al., 2018) according to the manufacturer’s recommendations with RNAscope Probe-Mm-Cxcl1 (407721; Advanced Cell Diagnostics) and RNAscope Probe-Mm-Pecam1 for ECs (316721; Advanced Cell Diagnostics). The signal was developed by tyramide signal amplification reagents (PerkinElmer). The fluorescent signals were captured with a confocal fluorescence microscope (Zeiss LSM800).
Primary astrocyte culture
All procedures for primary astrocyte cultures were performed as previously described (Sun et al., 2017). Briefly, hippocampal tissues from 4-wk-old mice were digested in 0.25% trypsin at 37°C for 20 min. Tissue homogenate was centrifuged with a 23% percoll solution at 4°C for 15 min, and the precipitate was retained. The precipitate was resuspended and preincubated with DMEM and 10% FBS for 15 min; then, cells were seeded onto plates coated with poly-D-lysine. Half of the medium was replaced every 3 d. After 10 d, culture plates were shaken continuously for 24 h at 37°C to eliminate microglia. The cells were split into new plates at a density of 30,000 cells/cm2 and incubated for subsequent experiment.
Viral injections were performed in Cdk5f/f and Cdh5-Cre;Cdk5f/f mice. rAAV-CaMKIIα-Cre-WPRE-pA (2.50 × 1012 viral particles ml−1) or rAAV-GFAP-Cre-WPRE-pA (5.54 × 1012 viral particles ml−1) mixed with rAAV-U6-Loxp-CMV-mCherry-Loxp-shRNA (Cxcr2, 1:1; 2.20 × 1012 viral particles ml−1) was injected bilaterally into CA1 (anteroposterior: −2.0 mm; mediolateral: ±1.5 mm; dorsoventral: −1.5 mm) with corresponding control virus. The sequence of Cxcr2 shRNA in AAV-Cxcr2-RNAi is 5′-CGAAATCCTGTTAAGGTAAACCTT-3′. Similarly, rAAV-Efla-DIO-GLT1-mCherry-WPRE-pA (5.43 × 1012 viral particles ml−1) was injected bilaterally into CA1 of 4-wk-old mice. All viruses were purchased from BrainVTA. Viruses were injected in a volume of 400 nl at 100 nl/min as previously described (Tan et al., 2019). To minimize tissue injury, the AAVs were delivered into the target region last for 15 min through a 10–20-μm diameter tip of a glass microelectrode with a nanoliter injector (WPI).
For systematic delivery of AAV-BR1 (plasmid provided by Jakob Körbelin, University Medical Center Hamburg-Eppendorf, Hamburg, Germany), 4-wk-old mice were i.v. injected in the lateral tail vein with AAV-BR1-CAG-iCre-2A-EGFP or AAV-BR1-CAG-EGFP or pAKD-CMV-bGlobin-EGFP-H1-shRNA (Cxcl1; 1.6 × 1011 genomic particles/mouse). The sequence of Cxcl1 shRNA in BR1-Cxcl1-RNAi is 5′-CCACTGCACCCAAACGAAGTCATA-3′. Further detections were performed 3 wk after virus injection.
Data are presented as means ± SEM. Unpaired two-tailed Student’s t test was used for datasets including two independent groups. One-way ANOVA, followed by Tukey’s post hoc test, was applied to analyze different groups when there were more than two. Two-way ANOVA (genotype × trial) was used to analyze groups with two factors. P < 0.05 was considered to be statistically significant.
Sequence data that support the findings of this study have been deposited in the National Center for Biotechnology Information Sequence Read Archive with the accession codes SRR9637648, SRR9637649, SRR9637650, SRR9637651, SRR9637652, and SRR9637653.
Online supplemental material
Fig. S1 shows that ablation of endothelial Cdk5 increased sensitivity to PTZ-induced epilepsy. Fig. S2 shows that dysfunction of astroglial GLT1 contributes to neuronal excitability and epilepsy. Fig. S3 shows that endothelial CXCL1 regulates astrocytic glutamate uptake via astroglial CXCR2 receptor. Video 1 shows an episode of a spontaneous behavioral seizure observed in 16-wk-old Cdh5-Cre;Cdk5f/f mice.
We thank Dr. Jakob Körbelin for plasmids pXX2-187-NRGTEWD, pAAV-CAG-iCre-2A-EGFP, and pAAV-CAG-eGFP. We also thank Dr. Ralf Adams for the Cdh5-CreERT2 line.
This work was supported by the National Key Research and Development Program of China (2016YFE0125400 to F. Han), the National Natural Science Foundation of China (81730101 to F. Han and 81673415 to Y.-m. Lu), and Nanjing Medical University Science and Technology Development Fund (NMUD2018001 to Y.-m. Lu).
The authors declare no competing financial interests.
Author contributions: X.-x. Liu, L. Yang, and L.-x. Shao designed the study, performed experiments, analyzed the data, and wrote the manuscript. Y. He prepared primary cultured ECs and performed experiments. G. Wu and Y.-h. Bao conducted EEG recording and analyzed data. N.-h. Sun and D.-y. Chen performed experiments using in situ hybridization. H.-s. Chen performed bioinformatics analysis of RNA sequencing data. N.-n. Lu, D.-m. Gong, Y.-p. Lu, and T.-t. Cui performed additional experiments. K. Fukunaga and W.-x. Shi reviewed the data. Z. Chen, F. Han, and Y.-m. Lu supervised the work, designed the research, and wrote the paper.
X.-x. Liu, L. Yang, and L.-x. Shao contributed equally to this paper.