Of all the anterior hypophyses tested, those of the human produced the most marked diabetogenic action in the dog with its pancreatic tissue reduced to 4 gm., and in the hypophysectomized and pancreatectomized toad. The human hypophysis also produced diabetogenic action in the normal dog on daily doses of 1.26 mg. per kilo per day for 2 days.
The hypophysectomized dog with its pancreas reduced to 4 gm. is very sensitive to the anterior hypophyseal diabetogenic action and is the best test animal for demonstrating such action in mammals.
The anterior hypophysis of man, toad, rat, and chicken produces in such animals a diabetogenic action with doses of from 10 to 15 mg. per kilo per day. The bovine anterior hypophysis has identical action in 20 mg. doses. That of canine origin was much less active in a few though inconclusive experiments.
It was impossible to demonstrate a diabetogenic action with either guinea pig hypophysis or with that of fish probably because insufficient doses were injected.
The diabetogenic action was not obtained by the injection of other organ extracts of toads, dogs and oxen, of corticosterone (30, 40, and 60 mg. in 4 days) or of desoxycorticosterone (80 mg. and 200 mg. in 4 days).
The toad (Bufo arenarum Hensel), deprived of its hypophysis and pancreas is the most sensitive biological reactor for testing the diabetogenic action. In this animal the diabetogenic action of anterior hypophyses from varied sources decreased in the following order: man, dog, toad (Bufo arenarum Hensel), white rat, guinea pig, chicken (whole hypophysis), ox, serpent (Constrictor constrictor (L.)), the fish "corvina" Micropogon opercularis (Quoy and Gaimard, 1824), and "merluza" Merlucius hubbsi (Marini, 1933).