The applicability to the non-hemolytic streptococci of the technique used for the extraction of a soluble precipitating substance from the hemolytic streptococci is demonstrated.

With this substance as antigen, it is demonstrated by means of precipitation and complement fixation that the non-hemolytic streptococci form an antigenically distinct, but entirely heterogeneous group.

Such cross-reactions as do occur within this group are not useful in the systematic classification of these organisms.

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