How platelets are produced by megakaryocytes in vivo remains controversial despite more than a century of investigation. Megakaryocytes readily produce proplatelet structures in vitro; however, visualization of platelet release from proplatelets in vivo has remained elusive. We show that within the native prenatal and adult environments, the frequency and rate of proplatelet formation is incompatible with the physiological demands of platelet replacement. We resolve this inconsistency by performing in-depth analysis of plasma membrane budding, a cellular process that has previously been dismissed as a source of platelet production. Our studies demonstrate that membrane budding results in the sustained release of platelets directly into the peripheral circulation during both fetal and adult life without induction of cell death or proplatelet formation. In support of this model, we demonstrate that in mice deficient for NF-E2 (the thrombopoietic master regulator), the absence of membrane budding correlates with failure of in vivo platelet production. Accordingly, we propose that membrane budding, rather than proplatelet formation, supplies the majority of the platelet biomass.

Since megakaryocytes (Mks) were identified by Wright (Wright, 1906; Wright, 1910) as the source of platelets (Plts), the cellular mechanism of Plt production has been a matter of sustained debate (Becker and De Bruyn, 1976; Behnke, 1969; French, 1967; Ihzumi et al., 1977; Italiano et al., 1999; Junt et al., 2007; Nishimura et al., 2015; Radley and Haller, 1982; Radley and Hartshorn, 1987; Zucker-Franklin and Petursson, 1984). When making his seminal discovery, Wright also observed long pseudopodial processes (known as proplatelets [proPlts]) that extended from Mks into the peripheral circulation, he speculated that these could be released into the blood flow to yield circulating Plts (Wright, 1906; Wright, 1910). Although it has been reported that Plts can be shed as a consequence of explosive cell death during inflammatory stress (Kowata et al., 2014; Nishimura et al., 2015; Yamada, 1957), the process of proPlt formation has been observed during both in vitro differentiation studies (Choi et al., 1995; Italiano et al., 1999) and intravital imaging experiments (Junt et al., 2007; Lefrançais et al., 2017; Zhang et al., 2012). Thus, proPlt formation has been broadly accepted as the universal Plt-forming mechanism. However, the release of Plts from proPlts has not yet been directly observed in vivo; thus, the extent to which proPlts contribute to the Plt biomass remains unclear. Understanding the physiological Plt-forming process will resolve a fundamental biological question of historical standing and may help the field to overcome the in vitro scalability problem that limits cost-effective Plt production for clinical use (Ito et al., 2018; Nakamura et al., 2014; Thon et al., 2014).

We have developed quantitative 3D and 4D imaging methods that enable the behavior of Mks to be studied in their native environment at the scale of the whole organ. Thus, we could visualize Mks and Plt formation from the initiation of hematopoiesis in the early embryo, through fetal development, to adult life. From these studies, we found that while proPlt formation was a relatively rare phenomenon at all stages studied, robust Plt production was observed via plasma membrane budding. In support of the vital role of budding, we found that the severe thrombocytopenia observed in Nfe2−/− mice was not associated with loss of in vivo proPlt formation, as had been proposed on the basis of in vitro assays (Lecine et al., 1998), but correlated with failure of membrane budding.

Our findings indicate that the output of membrane budding can account for the maintenance of the steady-state circulating Plt mass through the consistent delivery of nascent Plts directly into the circulating blood. In addition, this occurs without signs of proPlt formation or cell death. Furthermore, in the adult bone marrow (ABM), we observed membrane budding in Mks that have previously been classified as sessile, suggesting that the role for these cells in Plt production have been significantly underestimated.

Intravital imaging studies of Mks in the ABM have established the existence of proPlt formation and explosive fragmentation as mechanisms of Plt production (Junt et al., 2007; Nishimura et al., 2015); we sought to understand which mechanisms are used during prenatal life. Primitive (non–stem cell–derived) Mks appear in the yolk sac between embryonic day 8.5 (E8.5) and E10.5 (Potts et al., 2014, 2015; Tober et al., 2007) and in vitro are capable of generating classic proPlts composed of Plt-sized swellings interconnected by fine shafts (Fig. 1 A). Because these primitive Mks undergo rapid terminal differentiation in vitro and Plts rapidly expand in number from E9.5 to E10.5 (Potts et al., 2014; Tober et al., 2007), we reasoned that if proPlts were producing these Plts they would be abundant at these stages in vivo. 3D imaging of the intact yolk sac revealed that proPlt-like structures were rare (2.9% ± 3.5%; Fig. 1, B and C); surprisingly, a high frequency (35% ± 14%) of Mks with Plt-sized buds directly attached to the plasma membrane were observed in vivo (Fig. 1, B and C). In contrast, only proPlt formation was observed by primitive Mks when cultured in vitro (Fig. 1 D), suggesting that an alternative mechanism of Plt production might occur in vivo in the midgestation embryo.

We next investigated whether membrane budding also occurs from conventional (highly polyploid) Mks in the fetal liver (FL) and thus represents a more general prenatal Plt-forming mechanism. Using scanning electron microscopy, we observed relatively large cells with buds at the plasma membrane (Fig. 1 E). Transmission electron microscopy confirmed that buds were associated with polyploid Mks and that buds contained organelles (Fig. 1 F). Studying Plt formation using conventional 2D methods (such as transmission electron microscopy) limits the information that can be acquired for any given Mk. The FL is a soft tissue that can be easily dissected and optically cleared for 3D imaging, making it a tractable model for studying Mks in their natural environment. E13.5 FL were isolated and manually sliced into ∼500-µm sections to allow acquisition of full-volume images of intact Mks in situ at high magnification. This approach provided adequate spatial resolution for the detection of fine structures such as filipodia, proPlts, and membrane buds. Conversion of these large-volume images into high-content datasets through the creation of surface objects based on CD41 expression (identifying both Mks and Plts) allowed the extraction of multiple quantifiable parameters such as protein expression, DNA content, and cell size (Video 1). In combination with behavior classification, this approach allowed us to undertake the first unbiased in situ exploration of Mk behavior (Fig. 2 A and Fig. S1). We found that (a) although Mks readily undergo proPlt formation in vitro (Fig. 2 Bi), proPlt-displaying Mks were rare in vivo (1% ± 2%); however, 53% ± 8% of Mks in the E13.5 FL displayed membrane buds (Fig. 2 Bii); (b) circulating Plts, free Plts in the FL, and Mk-associated buds exhibited similar size ranges (Fig. 2 C); (c) free Plts and buds contained the α-granule–associated proteins von Willebrand factor (VWF) and Plt factor 4 (Fig. 2 D); (d) buds exhibited Plt-like patterning of the key cytoskeletal proteins (Pertuy et al., 2014; Schwer et al., 2001; Thon et al., 2010, 2012) F-actin, myosin heavy chain 9, and β1-tubulin (Fig. 2 E); and (e) the ploidy of budding Mks ranged from 2N to 16N, which was not significantly different from those that displayed no evidence of Plt production (Fig. 2, Fi and Fii). In contrast, the few instances of proPlt-forming Mks that were observed had a significantly lower ploidy range (2N–8N; Fig. 2 Fiii, P < 0.05 with Fisher’s exact test and Bonferroni’s correction). Thus, membrane bud formation was concurrent with Mk maturation (endoduplication).

To determine whether Plt buds are produced by the attached Mk in the FL, the lineage tracking Confetti system (Snippert et al., 2010) was used in combination with the Mk-specific Pf4-Cre mouse line (Tiedt et al., 2007). In the Pf4-Cre:Confetti system, Cre is continuously expressed in Mks, causing repeated inversion of the reporter cassettes which, depending on the ploidy of the Mk at time of activation, results in four resolvable Confetti configurations (Fig. 3 A and Fig. S2; see Materials and methods). To assess the developmental relationship between Mks and their associated buds, we scored their Confetti configurations. Of 732 buds associated with 184 Mks, 98% were configuration matches (Fig. 3, B and C). These data favor the model that membrane buds are derived from the attached Mk.

To determine whether the presence of membrane buds on Mks was caused by apoptosis, we stained whole-mount preparations of FL to detect the presence of active (cleaved) caspase-3, the effector of apoptotic cell death. Analysis of positive control samples treated with Camptothecin (a potent inducer of apoptosis) confirmed our ability to detect apoptosis if present, but we found that very few cells in the fresh liver were undergoing cell death (Fig. 3 Di). Quantification of active caspase-3 in the general Mk and Plt populations (Fig. 3 Dii) and within budding Mks (Fig. 3 Diii) revealed no evidence of apoptosis in situ. This is consistent with the observation that apoptosis must be restrained for effective Plt production (Josefsson et al., 2011).

To track the fate of membrane buds, we developed an ex vivo protocol that enabled 4D (3D + time) imaging of Mks within thick sections of FL. The principle of this in toto strategy is that to understand in situ Plt production, Mks must be preserved within an organotypic architecture. To this end, sections of FL were maintained in a serum-free medium widely used to induce proPlt formation (Norol et al., 1998), but without exogenous growth factor supplementation. This meant that any Plt-inducing factors were supplied by the FL explant. 3D images were acquired at 3–5-min time resolution for ≤24 h, and cellular behavior (Plt-forming mechanism and death) was manually annotated. After the generation of surface object tracks, we derived signature traces of changes in cell volume and sphericity that accompanied these behaviors.

Of the 122 Mks analyzed: 57% did not engage in Plt formation or undergo death, these cells maintained stable cell volume and sphericity (Fig. 4 Ai); 2% underwent a pyroptosis-like cell death (Lamkanfi and Dixit, 2010), during which cellular volume rapidly expanded before an acute collapse and cessation of movement (Fig. 4 Aii); 3% underwent proPlt formation, initiation of which resulted in a concomitant increase in volume and decrease in sphericity as proPlts were extended (Fig. 4 Aiii and Video 2); and direct release of Plts via plasma membrane budding was observed in 38% of Mks, not accompanied by marked change in volume or sphericity (Fig. 4, Aiv and B; and Video 3). Importantly, none of the 143 observed budding events gave rise to proPlts. The volume of nascent Plts derived from budding was similar to that of free Plts (Fig. 4 C), and we noted that Plt release occurred through the liberation of single or multiple Plts in a single shedding event (Fig. 4 D). Each Mk was tracked for 1–15 h (7 h median), and the time observed after the release of the first bud was 1–15 h (4 h median). No budding Mks were observed to undergo cell death (the censoring event being the end of experiment or exit from the field of view), which is consistent with the absence of apoptosis in budding Mks in vivo (Fig. 3 D). As is the case in the ABM (Zhang et al., 2012), FL Mks were in direct association with the vasculature, and VWF-loaded buds were frequently observed protruding through the endothelial lining (Fig. 4 E and Video 4), thereby providing opportunity to deliver Plts directly into the peripheral circulation.

To test whether Plt production via membrane budding is restricted to the prenatal organism, we studied Mks in the ABM using previously described two-photon intravital imaging of the calvarium (Duarte et al., 2018; Hawkins et al., 2016). The behavior of hematopoietic cells in calvarial bone marrow (BM) accurately reflects that of other long bones (Koechlein et al., 2016; Reismann et al., 2017) but has the advantage that the surrounding bone is thin enough to allow visualization of the marrow without the need to manipulate the bone (Hawkins et al., 2016), permitting 4D imaging of the entire marrow. We found that large Mks were highly abundant within the BM (Fig. 5 A), and of 442 Mks observed in 3D z-projections, 1% (4 of 442) had associated proPlts, with the remaining Mks appearing sessile, with either a classic circular or complex morphology (Fig. 5, B and C).

The scope of past Mk intravital imaging experiments has been constrained by the necessity to select regions of interest, imposing restrictions on either the number of cells that can be observed or the duration of observation. We circumvented this problem by collecting 3D tiled images of the entire calvarial BM at sub-4-min intervals for 6 h. Using this approach, we were able to undertake unbiased tracking of the steady-state behavior of all Mks resident in the BM in parallel (Video 5). Of the 825 Mks observed, only 2% (18 of 825) underwent proPlt formation (Fig. 5 D). We observed that proPlt formation took 18–336 min (mean 103 min) to complete, measured from the time of initiation to detachment (Fig. 5, E and F; and Video 6). 7% (58/825) of Mks generated thick extensions that did not develop into proPlts (Video 7), and 91% of Mks appeared to be sessile (no observed proPlt formation or development of thick extensions; Fig. 5 D).

ProPlt-forming Mks in the BM underwent marked decreases in volume through the period of observation (Fig. 5 G). If proPlt formation was the major driver of in vivo Plt biogenesis, it is expected that over a 6-h period the vast majority of Mks would have undergone proPlt formation (Junt et al., 2007) and so would have exhausted their cytoplasmic volume. Contrary to this prediction, the volume of non–proPlt-forming/sessile Mks remained relatively stable (Fig. 5 H). Based on deductive reasoning, it has been estimated that each Mk is capable of producing a total of 100–4,000 Plts; thus, the Mk population is thought to provide the organism with tens of millions of new Plts per hour (Fuentes et al., 2010; Kaufman et al., 1965; Lefrançais et al., 2017). Given the hourly rate of Plt formation, these data indicate that in vivo Plt production does not result in acute Mk exhaustion.

We reasoned that because instances of proPlt formation were rare (Fig. 5, C and D) and took >1 h for proPlt detachment to occur (Fig. 5, E and F), this process was unlikely to be sufficient to maintain steady-state Plt numbers. Given our observations in the prenatal organism, we hypothesized that if membrane budding is a conserved feature of Mk biology, then Mks previously classified as sessile could be candidates for suppliers of Plt production.

Unlike the FL explant system, capturing potential budding events in live adults is complicated by the high velocity of the circulating peripheral blood. To account for this, we adopted a focused high-time-resolution approach, for which 45 sessile Mks (which included both circular Mks and those with complex morphology) were imaged in 3D (15–30-µm range in z-axis) at a 2–3-s time resolution for 10–15 min. To distinguish Mk-associated buds from passing Plts, we applied the criteria developed from our FL studies: classification as a bud required continuity between the Mk and the attached object. If this condition was not met, the object was classified as a passing Plt (Fig. S3). Of the 45 sessile Mks investigated, a strikingly high proportion (84%) were observed releasing Plts via membrane budding directly into the circulating blood without the formation of a proPlt intermediary (Fig. 5, I and J; and Video 8). Importantly, none of the budding events observed resulted in proPlt formation. Plt production via budding occurred at a rate of 2 Plts/min (Fig. 5, K and L), which extrapolates to a production rate of 120 Plts per Mk/hr. Consistent with membrane buds being poised for release as nascent Plts, the size of buds was similar to that of Plts in the peripheral circulation (Fig. 5 M).

Next, we assessed to what extent Mk budding in the calvarial BM is representative of that in other Mk-containing sites. We performed extensive investigations of Mk distribution and morphology in all major sources of BM (pelvis, long bones, ribs and sternum, skull, and spine). Mks were similarly frequent in all sources of BM (Fig. 6 A). Not only was the frequency of Mks higher in the BM compared with spleen and lungs (Fig. 6 B), but by quantifying Mk numbers in the majority of bones within the skeleton, we found that the BM harbored the vast majority of all the Mks in the adult: 79% in the BM, 13% in the spleen, and 8% in the lungs (Fig. 6 C and Fig. S4). To generate a broad snapshot of Plt-forming morphologies, Mks were scored in thick sections of the femur, pelvis, spleen, and lung (Fig. 6 D and not depicted). This revealed that at any given time ∼1% of Mks were undergoing proPlt formation but ∼50% were budding (Fig. 6, E–G). Consistent with our intravital imaging data (Fig. 5 M), membrane buds in the BM were similar in size to circulating Plts (Fig. 6 H). The exception to this trend was the lungs, which contained fewer Mks (Fig. 6 C), but a large proportion were undergoing proPlt formation (Fig. 6 I). This appeared to occur mostly from Mks located within the lung vasculature. We noted that membrane buds in the BM and spleen contained F-actin structures that were continuous with the F-actin present in the peripheral zone of the attached Mk (Fig. 6, J–L), suggesting the involvement of the cytoskeletal cortex in Plt formation and/or release. In contrast, discrete F-actin structures were absent in Mks undergoing proPlt formation in the lungs (Fig. 6 M).

Disassembly of peripheral zone microtubules occurs as proPlt-forming BM Mks enter the lumen of the vasculature (Brown et al., 2018). This process is morphologically reminiscent of uropod elongation that has been observed to accompany the migration of multiple hematopoietic lineages both in vivo and in vitro (Hyun et al., 2012; Renkawitz et al., 2019). Leveraging our long-term intravital imaging data of the calvarial BM, the outcome of in vivo proPlt formation was explored. We observed that 20% of proPlt-forming Mks released large fragments into the circulation, and 60% appeared to either exhaust their cytoplasm or fully enter into the peripheral circulation without proPlt release (Fig. 7 A). To resolve these possibilities, we imaged Mks at 15 frames/s and found clear evidence that polyploid Mks could exit from the BM space during proPlt formation (Fig. 7 B and Video 9).

The sum of evidence indicates that membrane budding accounts for a major proportion of the Plt biomass during prenatal and adult life. Furthermore, these data suggest that proPlt formation and membrane budding are likely to be mechanistically distinct processes. To test this, we investigated how budding and proPlt formation were affected in Nfe2−/− mice. NF-E2 (nuclear factor, erythroid 2) is a critical transcriptional regulator of in vivo Plt production (Levin et al., 1999; Shivdasani et al., 1995); in the absence of NF-E2, Mks are present in normal numbers in embryonic and adult mice, but they lack circulating Plts (Lecine et al., 1998; Potts et al., 2015; Shivdasani et al., 1995). Because FL-derived Nfe2−/− Mks do not generate proPlts in vitro (Lecine et al., 1998), it has been suggested that thrombocytopenia in Nfe2−/− mice is likely caused by failure of proPlt formation in vivo. Given that our findings brought into question the contribution of proPlt formation to the maintenance of Plt biomass, we investigated the in vivo Plt-forming processes affected in thrombocytopenic Nfe2−/− mice.

At E14.5, Nfe2−/− circulating Plts were 1% of Nfe2+/+ numbers (Fig. 8 Ai). At preceding developmental stages in the yolk sac (Fig. 8 Aii) and the FL (Fig. 8, Aiii–D), the frequency of Mks undergoing proPlt formation was not significantly different between Nfe2+/+ and Nfe2−/− samples; however, membrane budding was reduced to 7 and 17% of wild-type numbers, respectively. These data are consistent with membrane budding being a major contributor to the Plt biomass.

Nfe2−/− mice die in early neonatal life (Shivdasani et al., 1995), but Nfe2−/− Mks are produced in the adult after transplantation of FL cells (Lecine et al., 1998). To investigate whether defective thrombopoiesis in the Nfe2−/− adult hematopoietic system was associated with failure of Mk membrane budding, we generated adult hematopoietic chimeric mice. Nfe2+/+ or Nfe2−/− E13.5 FL (GFP) were transplanted into lethally irradiated mice that ubiquitously expressed GFP. After 4–5 wk, peripheral blood was taken to measure hematopoietic chimerism in circulating Plts and RBCs; BM, spleen, and lungs were harvested for large-scale confocal analysis to assess the Plt-forming morphologies of donor cell–derived (GFP) Mks (Fig. 9 A). In recipient mice transplanted with Nfe2+/+ cells, donor-derived reconstitution accounted for the vast majority of the circulating Plt and RBC pools (Fig. 9 Bi). In contrast, although Nfe2−/− recipient cells successfully reconstituted RBCs, they exhibited significantly reduced reconstitution of the Plt compartment. The vast majority of RBCs, however, were derived from Nfe2−/− donor cells: only the recipients’ own GFP+ cells had contributed to Plts (Fig. 9, Bii). Thus, as expected, Plt production from Nfe2−/− Mks was dramatically impaired. Assessment of donor-derived Mks in the BM and spleen revealed a modest increase in proPlt formation by Nfe2−/− cells, but the frequency of Mks with membrane buds was significantly reduced (Fig. 9, Ci, Cii, D, and E). ProPlt formation in the lungs was not significantly different between Nfe2+/+ and Nfe2−/−, but membrane budding was almost absent (Fig. 9, Ciii and F). Of note, free proPlts and proPlt fragments were observed in both E13.5 Nfe2+/+ and Nfe2−/− fetuses and in adult mice reconstituted with both Nfe2+/+ and Nfe2−/− cells (Fig. 9, G and H). These data indicate that in both the fetus and the adult, the predominant effect of Nfe2 deletion in vivo is a severe reduction in membrane budding.

Transcriptome-wide changes in culture-derived FL Nfe2−/− Mks have been previously described (Chen et al., 2007; Fujita et al., 2013; Motohashi et al., 2010; Takayama et al., 2010); however, there are currently no investigations of changes that occur in Nfe2−/− primary Mks in vivo. Because proPlt formation is favored over membrane budding in vitro, we performed single-cell RNA-sequencing (scRNA-seq) on primary in vivo Nfe2+/+ and Nfe2−/− Mks from E13.5 FLs and donor-derived (GFP) ABM Mks from adult hematopoietic chimeras to understand the broad molecular changes that underpin the loss of membrane budding. Sequencing metrics, quality control, and downstream analysis are described in Table S2 and Fig. S5. Mks were identified with lineage signatures generated from Haemopedia mouse RNA-seq (Fig. S5; Choi et al., 2019).

The number of genes that were differentially expressed (DE) in Nfe2−/− Mks varied according to developmental stage: in E13.5 Nfe2−/− Mks, 337 genes were DE (157 genes down-regulated and 180 up-regulated); in ABM Nfe2−/− Mks, 1,046 genes were DE (623 genes down-regulated and 423 up-regulated; Fig. 10 A, Data S1, and Data S2). To identify genes likely to be involved in membrane budding in both the fetus and the adult, we investigated those genes that were commonly DE in both E13.5 FL and ABM Nfe2−/− Mks: 36 genes were commonly down-regulated and 79 were commonly up-regulated (Fig. 10, B and C, and Table S3 and Table S4). We performed a gene set test on genes previously identified as bound by NF-E2 and DE in Nfe2−/− versus Nfe2+/+ E13.5-derived in vitro–cultured Mks (Fujita et al., 2013). These gene sets were significantly DE in primary E13.5 FL Nfe2−/− Mks (false discovery rate <10−7), but not in the adult MKs (Fig. 10, Aii and B). Thus, the broad transcriptional changes that occur in Nfe2−/− are largely dependent on developmental stage.

Of genes that were commonly down-regulated, four (Tuba4a, Rgs18, Mef2c, and Cdc20) have previously been shown to be important for normal Plt production (Delesque-Touchard et al., 2014; Gekas et al., 2009; Strassel et al., 2019; Trakala et al., 2015). Gene ontogeny (GO) term enrichment analysis of commonly DE genes indicated that failure of membrane budding was accompanied by the up-regulation of genes associated with catabolic and metabolic processes (Fig. 10 Ci) and the down-regulation of genes associated with cell cycle transition, Mk differentiation, and cytoskeletal organization (Fig. 10 Cii and Table S5).

Our findings suggest that membrane budding is a major driver of in vivo Plt production and is conserved in both fetal and adult Mks. Furthermore, we show that in vivo proPlt formation and membrane budding are mechanistically distinct processes.

It has previously been problematic to reconcile the high rate of adult Plt production with the observation that the vast majority of Mks appeared inactive (Lefrançais et al., 2017). Implementation of an unbiased quantitative strategy to combine extensive organ-wide intravital analysis with rapid-time-resolution visualization of in situ Mk behavior has allowed us to propose a solution: rather than being sessile, a high frequency of Mks in multiple BM sites and the spleen are actively engaged in Plt production via membrane budding. In contrast to the temporally extended process of proPlt formation, budding is rapidly executed to deliver a consistent supply of Plts. Our findings provide a robust dataset to determine Plt production from Mks in vivo, based on the direct visualization of Plt production as opposed to quantitative deduction alone.

By considering the total number of Mks in the major ABM spaces and applying our experimentally observed frequency and rate of Plt production in the calvarial BM, we calculate that Plt budding from BM Mks alone could produce ∼3.4 × 107 Plts/hr, which accounts for 89% of the estimated 3.82 × 107 Plts/hr required to maintain stable circulating numbers (see Materials and methods). Because the in vivo Mk lifespan is unknown, the total number of Plts each individual Mk can produce in vivo remains unknown. We found that although only a small fraction of Mks in the adult are located in the lungs, the majority of these cells were undergoing proPlt formation. It therefore remains possible that Plt production via proPlt formation occurs in the lungs rather than the BM. However, because of the relatively low number of Mks in the lungs and the time required for fragmentation to occur (Lefrançais et al., 2017), in the wild-type mouse, proPlt formation alone cannot account for the number of Plts required to maintain the steady-state Plt mass (Table S6, Table S7, and Table S8). Although visualization of membrane bud formation and release was conclusively observed in FL explants, due to current technological constraints (z-stack acquisition rates) we are unable to track released membrane buds into the peripheral circulation of adult mice. Accordingly, our estimate of Plt production via membrane budding in the adult has the caveat that the rate of membrane bud release is assumed to be equal to the rate of membrane bud formation.

We did not observe Plt release directly from proPlts in the BM. However, we cannot discount the possibility that this process occurs in the peripheral circulation (Thon et al., 2012); the extent to which this contributes to the circulating Plt mass would require experimental clarification. Given the high rate of Plt production via budding that was observed in vivo, approaches to produce sufficient Plts for cost-effective clinical use would be greatly enhanced by identifying conditions that promote sustained membrane budding.

Our findings indicate that the cellular mechanism of Plt formation is exquisitely sensitive to the environment. In vitro, the vast majority of Mks form proPlts. However, in vivo we observed a different outcome: the majority of in vivo Mks undergo membrane budding. The idea of context dependence is supported by our observations from Nfe2−/− mice: Nfe2−/− FL-derived Mks have been reported to be defective in their ability to generate proPlts in vitro (Lecine et al., 1998). In contrast, our data (which represent the first exploration of the in vivo behavior of Nfe2−/− Mks) indicate that NF-E2 is dispensable for in vivo proPlt formation but is critical for membrane budding. Although we show that the formation, release, and fragmentation of proPlts occur in the absence of NF-E2 in vivo, we cannot be certain that the severe loss of circulating Plts in Nfe2−/− mice is attributable to the loss of membrane budding alone.

We observed that in addition to the possibility that in vivo proPlts give rise to Plts, and consistent with the observations of others (Brown et al., 2018; Lefrançais et al., 2017), proPlt formation was associated with the exit of Mks from the BM space via a diapedesis-like process; this could result in the reported seeding of BM-derived Mks within the intravascular spaces of the lungs (Lefrançais et al., 2017). It will be of interest to determine whether membrane budding and proPlt formation do indeed represent alternative mechanisms of Plt production or whether in vivo proPlt formation is associated with other facets of Mk biology.

### Mice

Pf4-Cre (Tiedt et al., 2007), Nfe2−/+ (Shivdasani et al., 1995), Flk1-GFP (Jakobsson et al., 2010), YFP reporter (Srinivas et al., 2001), and mTmG mice (Muzumdar et al., 2007) were maintained on a C57BL/6 background, and R26R-Confetti (Snippert et al., 2010) on a 129/Sv background. Experimental procedures were approved by the Walter and Eliza Hall Institute Animal Ethics Committee. Developmental stages were determined morphologically by Theiler’s criteria. Timed matings were set up overnight, with the morning of a positive plug designated E0.5. All adult experimental mice were females 10–16 wk of age.

### Embryonic tissue collection

Embryos were dissected in prewarmed Dulbecco’s PBS (DPBS) with 7% FCS to isolate yolk sac or FL. Single-cell suspensions were generated by incubating tissues in 10% collagenase and dispase (Roche) for 45 min at 37°C, washing, and manually dissociated by trituration. FLs were manually dissected into cross sections using sharpened tungsten needles. Peripheral blood was collected from individual embryos in prewarmed calcium- and magnesium-free DPBS with 7% FCS and EDTA as previously described (Potts et al., 2015).

### ProPlt-forming assay

Either single-cell suspensions from whole FL or purified CD41highCD45 cells from the yolk sac were cultured in defined serum-free medium (Norol et al., 1998) with 100 ng/ml recombinant murine thrombopoietin (Peprotech) for ≤3 d at 37°C with 5% CO2.

### 3D confocal microscopy

Yolk sacs were fixed in 2% paraformaldehyde (PFA) for 20 min at room temperature; FLs were fixed in 2% PFA with 0.1% Tween-20 for 1 h at room temperature, sliced, blocked, and permeabilized in 10% FCS/0.6% Triton X-100. For GFP detection, samples were first stained with a purified chicken anti-GFP antibody and then with an anti-chicken–Alexa Fluor 488 antibody. Antibody staining steps were performed overnight at 4°C (antibodies listed in Table S8) with wash steps at room temperature over 2–4 h. Nuclei were visualized with DAPI. All liver samples were cleared in a glycerol gradient (5–80%) before confocal imaging. Images were captured using a Zeiss LSM780 inverted confocal microscope with a 40× oil-immersion objective lens. A pixel resolution of 1,024 × 1,024 was used with 2× averaging and laser power depth correction.

After dissection, ABM and spleen were fixed overnight in 4% PFA at 4°C. Lungs were inflated using either 4% PFA or 1.75% agarose, immersed in 4% PFA, and fixed overnight at 4°C. Bones were decalcified in 0.5 M EDTA, 4°C (Kusumbe et al., 2015). All samples were embedded in low-melting-temperature agarose (3%). A Leica VT1000 S vibratome was used to generate 250-µm sections of each tissue. After antibody staining (as above), data were acquired using a Zeiss LSM880 upright confocal microscope using λ scanning and spectral unmixing. Four to six full-length sections were used from each sample; samples were collected from three independent mice. Data from all samples were acquired using a 10× air objective lens and a 40× oil-immersion objective lens. A pixel resolution of 1,024 × 1,024 was used with laser power depth correction. Images were analyzed using Imaris 9 (Bitplane) and Prism 7 (GraphPad) software.

### FL 4D confocal microscopy

E13.5 liver slices were incubated in serum-free medium (Norol et al., 1998) with anti-CD41 (allophycocyanin conjugated) antibody at 37°C with 5% CO2 overnight to allow optimal antibody loading. Slices were transferred to glass-bottom 35-mm dishes (Ibidi) in fresh preacclimatized medium and overlaid with embryo culture-grade mineral oil (Sigma-Aldrich) for imaging. The Z-stack acquired was ∼50–80 µm; start and end positions were restricted to regions within the explanted organ; and cells on the coverslip were not included in the imaged region. Z-stacks were acquired every 3–5 min using a 633-nm laser for ≤15 h (in a humidified chamber at 37°C and 5% CO2) using a Leica SP8 microscope equipped with a high-resonance scanner. Data were captured at a 512 × 512-pixel resolution. Data presented are derived from eight independent experiments.

### ABM 4D intravital microscopy

Using either Pf4-Cre:YFP or Pf4-Cre:mTmG mice, intravital microscopy was performed with an Olympus FVMPE-RS two-photon upright microscope equipped with a motorized stage, two tunable infrared multiphoton lasers (Spectraphysics INSIGHT X3-OL 680–1,300-nm laser and Mai Tai HPDS-OL, 690–1,040-nm), and a resonance scanner. The signal was visualized with an Olympus FV30-AC25W 25× magnification water-immersion lens (1.05 NA) and nondescanned detectors. Collagen bone second harmonic generation (SHG) signal, Cy-5, Tomato, YFP, and GFP signals were detected as follows: excitation 1,125 nm − 660–750-nm emission (Cy-5); excitation 1,050 nm − 560–679-nm emission (Tomato); excitation 860 nm − 500–550-nm (YFP or GFP) or 415–485-nm (SHG) emission. For surgery, anesthesia was induced in mice with 5% isoflurane mixed with pure oxygen. This was gradually reduced to ∼2% as anesthesia stabilized. Surgery to attach the headpiece and imaging was performed as described previously (Hawkins et al., 2016). When required, vasculature was labeled by intravenous injection of 50 µl of 8 mg/ml 500-kD Cy5 Dextran (Nanocs). Acquired images were processed using Imaris (Bitplane) or Fiji/ImageJ (National Institutes of Health).

### Analysis of Pf4-Cre:Confetti FL

Transient expression of Cre in a diploid cell harboring one copy of the Confetti construct results in stable expression of nuclear GFP (nGFP), cytoplasmic YFP (cYFP), membrane CFP (mCFP), or cytoplasmic RFP (cRFP). Pf4-Cre is continuously expressed in Mks, so the diversity of colors per Mk is reduced owing to repeated cassette inversion, but generating four resolvable Confetti configurations: (a) no recombination (no fluorescence); (b) expression of cassette 1 (nGFP and cYFP); (c) expression of cassette 2 (cRFP and mCFP); and (d) activation in polyploid Mks (which harbor at least two Confetti copies) resulted in expression of both cassettes 1 and 2, (nGFP, cYFP, cRFP, and mCFP). Configurations (a) through (d) were distinguishable by microscopy using an antibody against GFP derivatives (nGFP, cYFP, and mCFP) and an antibody against cRFP. Because Plts are anucleate cells, expression of cassette 1 was determined by cYFP expression alone. mCFP was not detectable following sample preparation; thus, cRFP was used to identify cassette 2 expression.

### Quantitative analysis of imaging data

Only complete volumes of Mks and Plts were used for 3D and 4D data: any truncated cells were eliminated from further analysis. For 4D data, Mks within the imaging region for <1 h were excluded from analyses; annotation of tracks was stopped once the target cell migrated out of the field of view. ProPlts were defined as pseudopodial extensions that were equal to or greater than the diameter of the attached Mk. Surface objects for Mks and Plts were based on CD41 expression; nuclei surface objects were based on DAPI staining. The values for the relevant parameters were extracted from surface objects using Imaris software, exported as an Excel file, and processed in Prism software.

For both ex vivo FL and in vivo ABM 4D imaging, membrane buds were identified by interrogating both the z-projection and all z-positions within each frame. As shown in Fig. S3, buds were distinguished from passing Plts by the presence of structural continuity between the putative bud and Mk. This conservative approach likely resulted in the underestimation of the number of released Plts via budding, but eliminated the possibility of false-positive calls.

### Electron microscopy

Lobes from E13.5 FL were fixed at room temperature for 3–4 h in 2% PFA/2.5% glutaraldehyde/0.1 M sodium cacodylate buffer (pH 7.4), postfixed in 2% osmium tetroxide/0.1 M sodium cacodylate buffer, dehydrated through a graded series of ethanol, and then washed twice in 100% acetone. For transmission electron microscopy, dehydrated livers were infiltrated overnight in Spurrs resin. Ultrathin sections were cut with a diamond knife (Diatome) on a Leica Ultracut 7 ultramicrotome (Leica Microsystems) and picked up on copper grids. Sections were contrasted with methanolic saturated uranyl acetate and Reynold’s lead citrate. Images were captured using a JEOL 1011 TEM microscope. For scanning electron microscopy, after fixation and ethanol dehydration, lobes were fractured at room temperature followed by critical point drying. Liver pieces were returned to 100% ethanol and critical point dried (Leica EM CPD 300). Samples were then mounted on an aluminum stub, sputter coated in gold (Emscope sc500 gold sputter coater), and stored in a desiccator until imaged. A second gold sputter coat was applied immediately before image acquisition if charging had occurred. Images were captured on a JEOL NeoScope II SEM (Coherent Scientific).

### Flow cytometry

Single-cell suspensions were stained for 1 h on ice with combinations of antibodies as in Table S1; peripheral blood samples were stained for 20 min at room temperature. 7-Aminoactinomycin D (7AAD; Thermo Fisher Scientific) was used for viability discrimination. R26R-Confetti FL Mk and peripheral blood Plt samples were analyzed individually with 7AAD and anti-CD41, and then for expression of RFP, CFP, and GFP/YFP (detected in the same filter channel). Ploidy analysis samples were prepared as follows: single-cell suspensions were stained as above, then fixed in ice-cold 70% ethanol for 1 h, washed, and resuspended in 0.1% Triton X-100/0.5% DAPI/calcium- and magnesium-free DPBS.

### Confetti MEFs

MEFs were derived from R26R-Confetti/+ E13.5 embryos and passaged twice before cryopreservation. After recovery, MEFs at 50% confluence were treated with TAT-Cre (10 µM; generated in-house) for 5 h to induce Confetti recombination, then expanded for 8 d before purifying populations by flow cytometry. Cells were cytospun onto polylysine-coated slides, fixed with 2% PFA, and stained with anti-GFP and anti-RFP antibodies and DAPI as used in the whole-mount Pf4-Cre:Confetti imaging.

### Apoptotic active CASPASE-3 assay

Mks were analyzed in E13.5 FL slices by confocal microscopy for expression of cleaved active CASPASE-3 as a marker of apoptosis. As a positive control, liver slices were cultured in IMDM with 10% FCS, 0.1% β-mercaptoethanol, 1% l-glutamine, and penicillin/streptomycin, supplemented with 4 µM Camptothecin (Sigma-Aldrich; C9911) for 4 h at 37°C with 5% CO2. After fixation in 2% PFA with 0.1% Tween20 for 1 h, slices were processed as above and stained with anti-CD41 and anti-active CASPASE-3 antibodies. Slices were cleared using a glycerol gradient before confocal imaging on Zeiss LSM780 confocal as above.

### Adult tissue cellularity, Mk number, and circulating Plt number estimations

After removal of soft tissue, marrow was harvested from the following bones by crushing: all long bones (two femurs, two tibias, two radii, and two humeri), spine, pelvis, ribs and sternum, and skull. Cell suspensions of spleen were produced by crushing. Cell suspensions of lung were produced as previously described (Lefrançais et al., 2017). Absolute cell counts were determined using a hemocytometer, only cells excluding eosin were scored. The frequency of BM cells was determined using flow cytometry via the exclusion of small cells (to reduce contamination by peripheral blood) and 7AAD+ events. Mks were identified according to a TER119CD41highCD150+ immunophenotype. Values from flow cytometric analysis and hemocytometer counts were used to determine total Mk numbers. Adult peripheral blood was analyzed using an ADVIA blood counter, from which Plt concentration was determined (cells per liter); these values were adjusted to a previously described estimate of total blood volume per mouse (2.3 ml; Barbee et al., 1992).

### Hematopoietic chimera generation

Livers from five GFP E13.5 Nfe2+/+ or GFPNfe2−/− embryos were mechanically dissociated and transplanted into lethally irradiated (2 × 5 Gy) UBC-GFP+ recipients (0.38 embryo equivalents per recipient). Mice were screened at 4 wk for hematopoietic recovery (ADVIA) and GFP status (flow cytometry).

### Single-cell RNA sequencing (scRNA-seq)

Livers from eight E13.5 wild-type and six Nfe2−/− embryos were dissected, mechanically dissociated, filtered, and pooled by genotype. Samples were stained for CD41, CD42d, LIN (Ter119, B220). LIN CD41high CD42d+ Cells were sorted on an Aria Cell Sorter (Becton Dickinson), centrifuged, and counted. From hematopoietic chimeras that had received either wild-type or Nfe2−/− E13.5 livers, BM from the femurs, tibias, hips, and humeri were collected, pooled by genotype. Donor derived Mks were purified by flow cytometry according to a GFPTer119 B220 CD41high CD42D+ immunophenotype; after purity analysis and cell counting, collected cells were loaded into a Chromium Controller (10X Genomics; Table S4). Single cell 3′ v2 transcriptome libraries were obtained following manufacturer’s instructions and sequenced on NextSeq (Illumina; 75 bp, paired end).

### Bioinformatics analysis

FL Mks were purified from eight E13.5 Nfe2+/+ and six Nfe2−/− embryos. ABM Mks were collected from hematopoietic chimeras and pooled by genotype. Samples were stained for CD41, CD42D, Ter119, and B220; TER119B220CD41highCD42D+ cells were purified by flow cytometry. Single-cell 3′ libraries were generated using the Chromium platform (10X Genomics). Each sample was sequenced to 79–91% saturation. Genes detected in <10 cells were excluded. Adult samples were sequenced to ∼200 million reads and embryonic samples to ∼650 million reads, an average read depth of 150,000–210,000 reads/cell. Reads were processed using the 10X Genomics Cell Ranger Single Cell 2.2.0 pipeline as previously described (Zheng et al., 2017). Samples were split by genotype and developmental stage using indexes and then FASTQs generated from Illumina sequencing output were aligned to the reference genome using the STAR algorithm. Gene-barcode matrices were generated for each individual sample by counting unique molecular identifiers and filtering noncell-associated barcodes. This was then imported into the Seurat (v1.4.0.15) R toolkit for quality control and downstream analysis of our scRNA-seq experiment (Fig. S5; Macosko et al., 2015). All functions were run with default parameters, unless specified otherwise. Gene expression was log normalized to a scale factor of 10,000.

Hematopoietic lineage signatures were generated from Haemopedia mouse RNA-seq (Choi et al., 2019), using the top 100 (after removal of T cell receptor and Ig variants) genes for each of 13 lineages as defined in the collection, using the High Expression Search function at https://www.haemosphere.org/searches. Expression of signatures was compared with a competitive gene set test using Camera (Wu and Smyth, 2012). The most positively up-regulated signature was assigned to each cluster as its lineage (Fig. S5). One mutant embryonic cluster was assigned as erythrocytic but also scored high for the Mk signature. These cells were included as Mks for further differential expression analysis. The cells remaining were 170 Nfe2+/+ and 147 Nfe2−/− ABM Mks; 805 Nfe2+/+ and 1,138 Nfe2−/− E13.5 liver Mks (including 420 with erythrocyte character).

For differential expression analysis, counts were converted to log2 counts per million, quantile normalized, and precision weighted with the “voom” function of the limma package (Law et al., 2014). A linear model was fitted to each gene, and empirical Bayes moderated t statistics were used to assess differences in expression (Ritchie et al., 2015). Empirical Bayes moderated-t P values were computed relative to a fold-change cutoff of 0.5-fold using treat (McCarthy and Smyth, 2009). P values were adjusted to control the global false discovery rate across all comparisons with the “separate” option of the limma package, and genes of interest were selected that were found to be DE in the same direction in both comparisons. Data have been made available through NCBI’s GEO (Edgar et al., 2002) under accession GSE125579. For gene ontology analysis, genes that were identified as up- or down-regulated in both E13.5 and ABM Nfe2−/− Mks were entered into the Panther portal at http://geneontology.org/page/go-enrichment-analysis. Heatmaps were generated using Morpheus software. Where indicated, hierarchical clustering was performed using the “1 minus Person correlation” metric. Functional annotation of significantly DE genes was performed using the Panther portal (http://pantherdb.org/). Gene sets that are bound by NF-E2 and DE in in vitro cultures of E13.5-derived Nfe2−/− Mks were derived from Table S2 in Fujita et al. (2013). They were tested for differential expression in our primary sourced Nfe2−/− Mks using Fry rotation gene set test (Wu et al., 2010) in the limma R/Bioconductor (Ritchie et al., 2015) software package.

### Estimation of the systemic rate of Plt replacement and Plt production

Total number of Plts per mouse was determined by the sum of the number of circulating Plts (Fig. S4 C) and the number expected to be sequestered in the spleen (one third of total):
$Total platelets per mouse=circulating platelets+one third in spleen=2.9×109+1.45×109=4.35×109platelets.$
The Plt production rate required to maintain normal numbers of circulating Plts was determined by dividing the total Plt number per mouse by the time required for the replacement of all circulating Plts (114 h; Dowling et al., 2010; Mason et al., 2007):
$Platelets required per hour=(total plateletstotalreplacementtime)=4.35×109platelets114hours=3.82×107.$
The total number of sessile (non–proPlt-forming) Mks from all major BM sites, spleen, and lungs was calculated by multiplying the total number of Mks in each tissue by the fraction of “budding” and “nothing” cells (Fig. 6, C, E–G, and I):
$Sessile marrow megakaryocytes=336,358×(99100)=332,994cells.$
The total number of potential budding Mks in the BM was determined by multiplying the total number of sessile Mks by the fraction of observed budding Mks in the calvarial marrow (84%; Fig. 5 J):
$Budding marrow megakaryocytes =332,994×(84100)=279,715cells.$
The potential Plt production rate (per hour) by budding Mks in the BM was determined by multiplying the total number of budding Mks by the observed Plt release rate per minutes (2 per minute; Fig. 5 L) multiplied by 60 min:
$Marrow platelet production=(234,960cells×2per min)×60min=3.4×107platelets per h.$

### General statistical analyses

Prism 7 (GraphPad) was used for data analysis and graph production. Data are presented as mean ± SD and were analyzed using Student’s t test (two-way, unpaired). One-way ANOVA (using Tukey’s P value adjustment) was used for multiple comparisons. Differences were considered statistically significant when P < 0.05; designation of ns indicates differences were not significant. *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001. For the comparison of Plt formation and Mk ploidy, contingency analysis was performed using the two-tailed χ2 test; P > 0.05 was considered not significant. n was used to designate the number of independent experiments, and o for the number of observations.

### Online supplemental material

Fig. S1 shows validation of in situ ploidy quantification in the E13.5 FL from high-volume 3D imaging data. Fig. S2 shows validation of the Pf4-Cre:Confetti system to determine the origin of Mk membrane buds. Fig. S3 shows the criteria used for membrane bud identification in Pf4-Cre:YFP ABM. Fig. S4 shows the cellularity and Mk abundance in major Mk-containing adult organs. Fig. S5 shows the scRNA-seq quality control metrics and lineage assignment used. Video 1 illustrates the conversion of high-volume 3D images of the E13.5 FL into quantifiable datasets. Video 2 demonstrates that ex vivo proPlt formation is rare but can occur from Mks during E13.5 FL organotypic culture. Video 3 demonstrates that direct release of membrane buds occurs within the E13.5 FL environment without engaging proPlt formation. Video 4 shows an E13.5 FL Mk budding directly into the peripheral circulation. Video 5 shows an example of the large-scale 4D whole calvarial BM imaging datasets produced using multiphoton intravital imaging. Video 6 demonstrates that proPlt formation in the BM is a slow process. Video 7 shows that thick extensions from Mks with complex morphology do not produce proPlts. Video 8 shows three examples of Mks undergoing membrane budding in the ABM. Video 9 shows an Mk exiting from the BM space after proPlt formation. Table S1 shows the antibodies/protein detection reagents used in this study. Table S2 shows the metrics associated with generation of Mk scRNA-seq data. Table S3 shows commonly down-regulated genes in Nfe2−/− Mks versus Nfe2+/+ Mks. Table S4 shows commonly up-regulated genes in Nfe2−/− Mks versus Nfe2+/+ Mks. Table S5 is the complete list of GO biological process terms enriched from commonly down-regulated genes in Nfe2−/− Mks versus Nfe2+/+ Mks. Table S6 shows a summary of base values used to estimate Plt production. Table S7 shows the parameters used to estimate potential Plt yield from BM and lung Mks. Table S8 shows the comparison of Plt production via proPlt formation and membrane budding. Data S1 lists some of the different genes used. Data S2 lists the remaining genes used.

We thank Dr. Julie Sheridan and Professor Marnie Blewitt for critical comments on the manuscript and Dr. Sarah Best for assistance with lung experiments. We are grateful to Professor Alexander Medvinsky (Centre for Regenerative Medicine, Edinburgh, UK) and Dr. Kelly Rogers (Walter and Eliza Hall Institute, Melbourne, Australia) for discussions and generous provision of reagents, and to Associate Professor Sarah Ellis for assistance with electron microscopy. Next-generation sequencing was performed by Dr. Stephen Wilcox at the Ian Potter Centre for Genomics within the Walter and Eliza Hall Institute.

This work was supported by the Australian Research Council Stem Cells Australia program, National Health and Medical Research Council project (1128993, 1129012) and Program (1113577) grants, and Independent Research Institutes Infrastructure Support Scheme from the National Health and Medical Research Council (grant 361646), the Australian Cancer Research Foundation, and State Government of Victoria Operational Infrastructure Support. E.D. Hawkins and W.S. Alexander were supported by National Health and Medical Research Council fellowships; K.S. Potts by an Australian Research Council Strategic Research Australian Postgraduate Award studentship; and E.C. Josefsson by a fellowship from the Lorenzo and Pamela Galli Charitable Trust.

Author contributions: K.S. Potts, A. Farley, C.A. Dawson, J. Rimes, C. Biben, C. de Graaf, O.J. Stonehouse, A. Carmagnac, C. Anttila, D. Amann-Zalcenstein, S. Naik, M.A. Potts, P. Gangatirkar, E.C. Josefsson, W.S. Alexander, D.J. Hilton, and E.D. Hawkins designed and/or performed experiments and analyzed data; S. Taoudi conceived the study, designed and performed experiments, and analyzed data. All authors contributed to manuscript preparation.

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## Competing Interests

Disclosures: The authors declare no competing interests exist.

## Author notes

*

K.S. Potts, A. Farley, C.A. Dawson, and J. Rimes are co-first authors.

**

E.D. Hawkins and S. Taoudi are co-senior authors.

K.S. Potts’s present address is Deptment of Developmental and Molecular Biology, Albert Einstein College of Medicine, Bronx, NY.