Cholesterol, in 0.1 gram amounts, melted in a compact mass and inserted into the subcutaneous tissues of the back of the guinea pig, is slowly removed, as determined by chemical analyses. The rate of removal is probably greatly dependent upon the physical state of the cholesterol.
The quantitative estimation of cholesterol by the method previously described can not be shortened and simplified by the direct saponification of the tissues by means of 5 per cent. sodium alcoholate or a strong (50 per cent.) solution of sodium hydroxide, instead of saponifying the alcohol-ether extract.