Inhibitory KIRs Induce Capture of Target Cell HLA-C
NK cell
.
Target cell
.
Number of conjugates imaged
.
Percentage of conjugates exhibiting clustering of GFP at the immune synapse
.
Percentage of conjugates showing transfer of GFP to NK cell
.
YTS/KIR2DL1
221/Cw6-GFP
415
66
49 (74)
YTS/ECO
221/Cw6-GFP
207
2
5
YTS/MOCK
221/Cw6-GFP
122
2
2
YTS/KIR2DL1
221/Cw4-GFP
20
80
65 (81)
YTS/KIR2DL1
221/Cw6-TMG-GFP
89
76
48 (63)
YTS/KIR2DL1
221/Cw3-GFP
174
5
3
NK cell
.
Target cell
.
Number of conjugates imaged
.
Percentage of conjugates exhibiting clustering of GFP at the immune synapse
.
Percentage of conjugates showing transfer of GFP to NK cell
.
YTS/KIR2DL1
221/Cw6-GFP
415
66
49 (74)
YTS/ECO
221/Cw6-GFP
207
2
5
YTS/MOCK
221/Cw6-GFP
122
2
2
YTS/KIR2DL1
221/Cw4-GFP
20
80
65 (81)
YTS/KIR2DL1
221/Cw6-TMG-GFP
89
76
48 (63)
YTS/KIR2DL1
221/Cw3-GFP
174
5
3
Various transfectants of 721.221 were imaged when conjugated to various YTS transfectants as listed. Table lists the percentage of cell conjugates for which GFP-tagged HLA-C clustered at the intercellular contact and the percentage of cell conjugates for which HLA-Cw6-GFP transferred to the NK cell. Numbers in parenthesis denote the percentage of those conjugates exhibiting clustering of GFP at the immune synapse that also show transfer of GFP into NK cell.
