The V–J Recombination of  T Cell Receptor-γ Genes Is Blocked in Interleukin-7 Receptor–deficient Mice

IL-7R-deficient mice were established by replacing the exon 2 with a PGK–neo cassette as described (12). Animals heterozygous (+/−) and homozygous (−/−) for the IL-7R mutation were on the (129/Ola × C57BL/6)F₃ hybrid background. The age of fetuses was determined by scoring for the appearance of a vaginal plug and taking as day 0 the morning on which the mating plug was observed. All mice were maintained under the specific pathogen-free conditions in the Animal Center for Biomedical Research, Faculty of Medicine (The University of Tokyo).

Thymocyte genomic DNA was digested with HindIII or EcoRI restriction enzyme and electrophoresed through 0.7% agarose gel. The DNA was transferred to polyvinylidene difluoride filters (Immobilon; Millipore, Bedford, MA) and hybridized with ³²P-labeled probes. The following fragments were used as probes: Jγ1, a 1.1 kb StyI–HindIII fragment containing the Jγ1 segment and its 3′ flanking region of genomic DNA from KN6 (15); Jα1, a 3.5 kb EcoRI–HindIII fragment of TA28.1 (16); Jδ1, a 2.5 kb SacI fragment of pCDS17 (17); Jβ2, a 2.3 kb EcoRI fragment of mouse genomic Jβ region (18). To confirm equal loading of genomic DNA, the membranes were hybridized with a 1.3 kb KpnI fragment of mouse RAG-2 cDNA (19). Southern blots were analyzed and radioactivity was quantitated using a Bio-image Analyzer (Fujix BAS2000; Fuji Film, Tokyo, Japan). The percentage of rearranged alleles was calculated by normalizing with the radioactivity of the RAG-2 probe.

Thymocyte DNA was prepared from fetuses at day 17 of gestation and mice at 4 wk old. PCR was carried out in a 25 μl reaction mixture containing 0.5 ng template DNA (0.5 μg for TCR β genes), 50 pmol each primer, 200 mM each dNTP, and 2.5 U Taq DNA polymerase. For TCR γ genes, samples were amplified for 30 cycles of 45 s at 94°C, 2 min at 50°C, and 1 min at 72°C. For TCR δ and β genes, PCR was performed as described previously (20–23). The PCR products were electrophoresed in 3% agarose gel, blotted onto nylon membranes, and hybridized with ³²P-labeled oligonucleotide probes. PCR primers are as follows: Vγ1.1 and Vγ1.2, 5′-CTTCCATATTTCTCCAACACAGC-3′; Jγ2, 5′-ACTATGAGCTTTGTTCCTTCTG-3′; Jγ4, 5′-ACTACGAGCTTTGTCCCTTTGG-3′; 5′ RAG-2, 5′-CACATCCACAAGCAGGAAGTACAC-3′; 3′ RAG-2, 5′-GGTTCAGGGACATCTCCTACTAAG-3′. Vγ2, Vγ3, Vγ4, Vγ5, Jγ1, Vδ1, Vδ4, Vδ5, Jδ1, Vβ8.2, Dβ2, and Jβ2 primers were described previously (20–23). Oligonucleotide probes used are as follows: Jγ2, 5′-CAAATACCTTGTGAAAGCCCGAGC-3′; Jγ4, 5′-CAAATATCTTGACCCATGATGTGC-3′. Jγ1, Jδ1, and Jβ2 oligonucleotide probes were described previously (20, 22). Radioactivity was analyzed using the Bio-image Analyzer.

Total RNA was isolated using the AGPC method as described (24). RNA samples were treated with RQ-1 RNase-free DNase (Promega Corp., Madison, WI) to remove contaminating genomic DNA. Oligo (dT)-primed cDNA was prepared by Molony murine leukemia virus RNase H⁻ reverse transcriptase (GIBCO BRL, Gaithersburg, MD) at 37°C for 1 h. PCR was carried out for 25 cycles of 1 min at 94°C, 1 min at 50°C for hypoxanthine phosphoribosyl transferase (HPRT) or at 55°C for others, and 1 min at 72°C. The PCR products were electrophoresed in 3% agarose gel, blotted onto nylon membranes, and hybridized with ³²P-labeled probes. The following DNA fragments were used as probes: RAG-1, a 1.4-kb EcoRI fragment of mouse RAG-1 cDNA (19); terminal deoxynucleotidyl transferase (TdT), a 1.3-kb EcoRI–EcoRV fragment of mouse TdT cDNA, M16-1b (25); Ku-80, a 540-bp PCR fragment of Ku-80 cDNA; HPRT, a 350-bp PCR fragment of HPRT cDNA. The RAG-2 probe was described above. The following PCR primers are used: 5′ RAG-1, 5′-GCAATGAGGAAGTGAGTCTGGA-3′; 3′ RAG-1, 5′-CTGAGGAAGGTATTGACACGGA-3′; 5′ Ku-80, 5′-AGAGGACACTATTCAAGGGTAC-3′; 3′ Ku-80, 5′-AGACACTGGTACAATCGCTGAA-3′; 5′ TdT, 5′-ACTGCGACATCTTAGAGTCA-3′; 3′ TdT, 5′-CTTCCCCTTAGTCCTGTCAT-3′; 5′ HPRT, 5′-CTCGAAGTGTTGGATACAGG-3′; 3′ HPRT, 5′-TGGCCTATAGGCTCATAGTG-3′. Radioactivity was analyzed using the Bio-image Analyzer.

To examine whether the signal from IL-7R affects the V–J recombination, we compared the rearrangement of the TCR γ genes between the thymocytes of IL-7R +/− and −/− mice. The thymocyte DNA from 4-wk-old mice was digested with HindIII or EcoRI, and a Southern blot was hybridized with the Jγ1 probe (Fig. 1,A, left). The Jγ1 probe allows the analysis of DNA rearrangements involving not only Jγ1 but also Jγ2 and Jγ3 gene segments (15). The ES cell DNA showed a 6.6-kb Jγ1, a 9.0-kb Jγ3, and a 11.7-kb Jγ2 germline fragment. The thymocyte DNA from IL-7R +/− mice showed decreased intensity of Jγ1 and Jγ2 germline fragments compared with embryonic stem (ES) cell DNA. Furthermore, a 3.6-kb Vγ1.2–Jγ2 and a 1.4-kb Vγ2–Jγ1 fragment was clearly detected in IL-7R +/− mice. Quantification of the radioactivity revealed that 71% and 74% of Jγ1 and Jγ2 alleles, respectively, were rearranged in thymocytes. Because γδ T cells are only 0.3% of total thymocytes (12), the majority of the Vγ1.2–Jγ2 and Vγ2–Jγ1 recombined fragments are derived from αβ T cells or precursor cells. On the other hand, no fragment derived from Vγ1.2–Jγ2 or Vγ2–Jγ1 recombination was detected in IL-7R −/− mice (Fig. 1 A). This result demonstrates that Vγ1.2–Jγ2 and Vγ2–Jγ1 rearrangements are almost completely blocked in αβ T cells in IL-7R-deficient mice.

We next examined adult and fetal thymus DNA by PCR with Vγ1.1+1.2, Vγ2, Vγ3, Vγ4, Vγ5, Jγ1, Jγ2, and Jγ4 primers. Thymus DNA revealed large amounts of PCR products with all the Vγ–Jγ primer pairs in IL-7R +/− mice. On the other hand, V–J rearrangement was greatly reduced in all the TCR γ genes in IL-7R −/− thymus; the signal of Vγ5–Jγ1 product was 150-fold reduced relative to IL-7R +/− mice, and those of Vγ1.1– Jγ4, Vγ1.2–Jγ2, Vγ2–Jγ1, Vγ3–Jγ1, and Vγ4–Jγ1 products were undetectable in IL-7R −/− mice (Fig. 2 A). Amplification with RAG-2 primers produced roughly the equal amount of PCR products in both IL-7R +/− and −/− thymus, suggesting that approximately the same amount of DNA was used in this analysis. These results support the data of Southern blot analysis and suggest that the V–J recombination is almost completely blocked in IL-7R-deficient mice not only in Vγ1.2 and Vγ2 genes but also in all the other Vγ genes.

We next analyzed the rearrangement of other TCR genes by Southern blot analysis. First, the Southern blot was hybridized with Jβ2 probe (see Fig. 1,A, middle). The ES cell DNA showed a 4.8-kb germline Jβ2 fragment. Thymocyte DNA showed decreased intensity of the Jβ2 germline fragment and smear patterns of Jβ2 recombined fragments in both IL-7R +/− and −/− mice. Quantification revealed that 81% and 69% of Jβ2 alleles are rearranged in IL-7R +/− and −/− thymus, respectively. Thus, the frequency of Jβ2 rearrangement is slightly decreased in IL-7R −/− mice compared with IL-7R +/− mice. Next, we hybridized a Southern blot with Jδ1 and Jα1 probes (see Fig. 1 B). A 6.8-kb Jδ1 and a 7.7-kb Jα1 germline fragment was detected in ES cell DNA. These were greatly reduced in the thymus because of deletion of the δ locus by Vα–Jα recombination in αβ T cells. Extra faint fragments and a smear pattern of Jδ recombined fragments were detected in IL-7R −/− mice as well as in IL-7R +/− mice. Thymocyte DNA from IL-7R −/− mice showed several Vδ–Dδ–Jδ and Dδ–Jδ recombined fragments at the comparable intensity with that from IL-7R +/− mice.

The rearrangement of TCR δ genes was further examined by PCR with Vδ1, Vδ4, Vδ5, and Jδ1 primers. In contrast with TCR γ genes, the signals of Vδ1–Dδ–Jδ1, Vδ4– Dδ–Jδ4, and Vδ5–Dδ–Jδ1 fragments were only slightly diminished (two- to eightfold reduction) in IL-7R −/− mice relative to IL-7R +/− mice (Fig. 2 B). Because IL-7R −/− thymus lacks γδ T cells (12), the Vδ–Jδ recombined fragments are probably derived from αβ T cells and precursor cells. Thus, the difference in the amounts of TCR δ products may be attributed to the presence and absence of γδ T cells in the thymus of IL-7R +/− and −/− mice, respectively. Collectively, TCR δ gene rearrangement seems not to be severely hampered in the IL-7R-deficient mice, supporting the data of Southern blot analysis.

Next, PCR amplification with Vβ8.2, Dβ2, and Jβ2 primers revealed six Dβ–Jβ and six Vβ–Jβ recombined fragments in both the IL-7R +/− and −/− thymus DNA (Fig. 2 C). These results demonstrate that IL-7R is not essential for both Dβ–Jβ and Vβ–Dβ–Jβ recombinations. It is recently reported that IL-7 supported Dβ to Jβ rearrangements but not Vβ to DβJβ rearrangement in fetal thymic organ culture of fetal liver precursor cells (26). However, our results do not support the notion that IL-7 may play some specific role on Dβ–Jβ recombination. All these results suggested that the rearrangements of TCR α, β, and δ genes take place normally in IL-7R-deficient mice.

RAG-1 and RAG-2 are indispensable for V–D–J recombination, and several other gene products such as TdT, Ku p70/80 and DNA-dependent protein kinase catalytic subunit are also involved in V–D–J recombination (27). To examine whether the signal from IL-7R affects the expression of these genes, we amplified cDNA prepared from adult thymocytes of IL-7R +/− and −/− mice by PCR with RAG-1, RAG-2, TdT, and Ku-80 primers, and hybridized with each cDNA probes (Fig. 3). The levels of RAG-1, RAG-2, TdT, and Ku-80 transcripts in IL-7R −/− mice were almost comparable to those of IL-7R +/− mice. These results suggest that the mutation of IL-7R did not inhibit the expression of RAG-1, RAG-2, TdT, and Ku-80 genes.

TCR γ genes are frequently recombined in αβ T cells (28). More than 70% of Vγ1.2 and Vγ2 alleles are recombined in total thymocytes. In this study, we used this phenomenon to examine whether TCR γ recombination is blocked in αβ T cell precursors of IL-7R-deficient mice. IL-7R-deficient mice had no detectable TCR γ recombination by Southern blot analysis. Furthermore, the recombination of all the Vγ genes was undetectable in fetal and adult thymi by PCR analysis. Thus, we demonstrated that the signal from IL-7R is indispensable for the V–J recombination of TCR γ genes in αβ T cell precursors. And it is highly possible that the TCR γ recombination is also blocked in γδ T cell precursors as well as in αβ T cell precursors. This would be certainly one reason why IL-7Rdeficient mice lack γδ T cells.

There are three significant features in our observation. First, this blockade is specific for TCR γ genes. The recombination of TCR α, β, and δ genes are not affected. In addition, the recombination of IgH and L chain genes is probably not hampered by the mutation, because the IL-7Rdeficient mice have decreased but certain numbers of surface IgM⁺ B cells in the periphery (12). Second, the recombination of all the Vγ genes is blocked. In a previous report, IL-7 induced the rearrangement of Vγ2 and Vγ4, but not Vγ3 or Vγ5 genes in fetal thymic organ culture of fetal liver precursors (2). In contrast, not only Vγ2 and Vγ4 but also all the other Vγ genes in the TCR γ1, γ2, and γ4 clusters are hampered to recombine in the mutant mice. Third, TCR γ gene recombination is blocked not only in γδ but also in αβ T cell precursors. These features suggest the presence of highly specific regulation for TCR γ gene rearrangement.

To explain the specific inhibition of TCR γ recombination in the IL-7R-deficient mice, one possibility can be considered. It is to suppose that the TCR γ locus may contain a specific cis-control element(s). One possible candidate for this element is TCR γ enhancers. Recently, it was reported that IL-7 induces the phosphorylation and DNA binding activity of Stat5 protein in T cells (29). Because each TCR γ enhancer contains a Stat5 binding sequence (30, 31), Stat5 may play a role on the regulation of TCR γ recombination. It is also possible that some unknown factor(s) other than Stat5 may specifically regulate the recombination of TCR γ locus.

We thank Drs. Y. Takagaki, H. Yamagishi, O. Koiwai, and Y. Hashimoto for probes and discussion; Ms. M. Sugimori and M. Iidaka for technical assistance; Dr. S. Takeda for critically reading the manuscript; and Drs. M. Fujiwara and T. Honjo for encouragement.