Interleukin 10 (IL-10) decreases production of IL-1, IL-6, and tumor necrosis factor alpha (TNF-alpha) in vitro, and neutralization of IL-10 in mice leads to elevation of the same monokines. We test here whether this monokine-suppressing property of IL-10 confers on it the capacity to protect mice from lipopolysaccharide-induced shock, a monokine-mediated inflammatory reaction. A single injection of 0.5-1 microgram of recombinant murine IL-10 reproducibly protected BALB/c mice from a lethal intraperitoneal injection of endotoxin. This result was obtained whether the IL-10 was administered concurrently with, or 30 min after the injection of endotoxin. The protective effect of IL-10 was reversed by prior injection of neutralizing anti-IL-10 antibodies, and correlated with a substantial decrease in endotoxin-induced TNF-alpha release. These data implicate IL-10 as a candidate for treatment of bacterial sepsis, and more generally as an effective antiinflammatory reagent.
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April 01 1993
Interleukin 10 protects mice from lethal endotoxemia.
M Howard,
M Howard
DNAX Research Institute, Palo Alto, California 94304.
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T Muchamuel,
T Muchamuel
DNAX Research Institute, Palo Alto, California 94304.
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S Andrade,
S Andrade
DNAX Research Institute, Palo Alto, California 94304.
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S Menon
S Menon
DNAX Research Institute, Palo Alto, California 94304.
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M Howard
DNAX Research Institute, Palo Alto, California 94304.
T Muchamuel
DNAX Research Institute, Palo Alto, California 94304.
S Andrade
DNAX Research Institute, Palo Alto, California 94304.
S Menon
DNAX Research Institute, Palo Alto, California 94304.
Online ISSN: 1540-9538
Print ISSN: 0022-1007
J Exp Med (1993) 177 (4): 1205–1208.
Citation
M Howard, T Muchamuel, S Andrade, S Menon; Interleukin 10 protects mice from lethal endotoxemia.. J Exp Med 1 April 1993; 177 (4): 1205–1208. doi: https://doi.org/10.1084/jem.177.4.1205
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