We compared T cell receptor (TCR) V-segment frequencies in human leukocyte antigen (HLA) identical siblings to sibling pairs who differ at one or both HLA haplotypes using four V beta-specific and one V alpha-specific monoclonal antibody. In every one of nine families HLA-identical sibs had the most similar patterns of V-segment frequencies in their peripheral blood, whereas totally mismatched sibs were, in general, the most dissimilar; HLA haploidentical sibs tended to be intermediate between the two groups. The degree of similarity among HLA-identical sibs was comparable to that observed among three pairs of identical twins suggesting that HLA is the major genetic component influencing TCR V-segment frequency. Consistent with this observation, it was found that the frequency of T cells expressing particular V beta segments was skewed towards either CD4+ or CD8+ cells indicating that T cells expressing some V beta genes may be positively selected primarily by class I or class II major histocompatibility complex proteins. Finally, it was observed that individuals who express the HLA class I specificity, B38, tend to express high levels of V alpha 2.3+ cells among their CD8+ T cells. These observations represent definitive proof that human V-segment frequencies are profoundly influenced by the HLA complex.
T cell receptor V-segment frequencies in peripheral blood T cells correlate with human leukocyte antigen type.
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B Gulwani-Akolkar, D N Posnett, C H Janson, J Grunewald, H Wigzell, P Akolkar, P K Gregersen, J Silver; T cell receptor V-segment frequencies in peripheral blood T cells correlate with human leukocyte antigen type.. J Exp Med 1 November 1991; 174 (5): 1139–1146. doi: https://doi.org/10.1084/jem.174.5.1139
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