The B6 anti-bm6 allospecific CTL response is strictly dependent on CD4+ cells when using LPS blasts as stimulator cells. Altering the N-linked carbohydrates on stimulator cells by use of the N-linked trimming glycosidase inhibitors 1-deoxymannojirimycin and swainsonine, or by treatment with bacterial neuraminidase, results in a restoration of the B6 anti-bm6 response in the absence of CD4+ cells. The extent of restoration is inversely correlated with the number of sialic acids present on N-linked glycans of stimulator cells.
N-linked glycan modification on antigen-presenting cells restores an allospecific cytotoxic T cell response.
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J J Neefjes, M L De Bruijn, C J Boog, J D Nieland, J Boes, C J Melief, H L Ploegh; N-linked glycan modification on antigen-presenting cells restores an allospecific cytotoxic T cell response.. J Exp Med 1 February 1990; 171 (2): 583–588. doi: https://doi.org/10.1084/jem.171.2.583
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