The T cell lymphoma line BW5147 has rearranged TCR alpha chain genes segments on both the homologous chromosomes: one is functional (V alpha 1) and the second (V alpha 16.1) is a pseudogene. The extreme 3' position of the V alpha 16.1 gene segment in the V alpha locus allows us to recognize rearrangements of most V alpha gene segments using the V alpha 16 probe as a marker. The absence of the genomic V alpha 16.1 gene fragment in mature thymocytes, antigen-specific T cells, and in more than two-thirds of the peripheral T cells suggests that most T lymphocytes rearrange both alpha loci. It appears that productive alpha chain rearrangement on one allele probably does not block a subsequent rearrangement on the other alpha locus.
In individual T cells one productive alpha rearrangement does not appear to block rearrangement at the second allele.
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V Kumar, J L Urban, L Hood; In individual T cells one productive alpha rearrangement does not appear to block rearrangement at the second allele.. J Exp Med 1 December 1989; 170 (6): 2183–2188. doi: https://doi.org/10.1084/jem.170.6.2183
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