We have previously demonstrated that H-2Kd-restricted CTL specific for HLA-CW3 or HLA-A24 can recognize synthetic peptides corresponding to residues 170-182 of the HLA molecules. Synthetic oligonucleotides encoding region 170-182 of CW3 or A24 were inserted into the influenza nucleoprotein (NP) gene. We demonstrate herein that P815 (H-2d) cells transfected with the NP-oligo recombinant genes are specifically lysed by HLA-specific Kd-restricted CTL clones. Our results imply that there must be a high degree of flexibility for the expression of T cell epitopes in different molecular contexts.
Recognition of oligonucleotide-encoded T cell epitopes introduced into a gene unrelated to the original antigen.
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G Chimini, P Pala, J Sire, B R Jordan, J L Maryanski; Recognition of oligonucleotide-encoded T cell epitopes introduced into a gene unrelated to the original antigen.. J Exp Med 1 January 1989; 169 (1): 297–302. doi: https://doi.org/10.1084/jem.169.1.297
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