We have previously reported that the urine of febrile humans contained large quantities of an inhibitor of IL-1-induced murine thymocyte proliferation that was a glycoprotein between 30 and 40 kD in size. In the present study this factor has been purified to homogeneity using a sequence of eight purification steps (ammonium sulfate precipitation, ion exchange chromatography, molecular sieve chromatography, hydrophobic affinity chromatography, hydroxylapatite chromatography, fast protein liquid chromatography, and two HPLC steps). SDS-PAGE analysis indicates that the purified material is a 38-kD molecule. Evidence based on a partial amino acid sequence analysis as well as enzyme studies indicates that this inhibitor is a type of human DNase I.
A human urine-derived interleukin 1 inhibitor. Homology with deoxyribonuclease I.
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D L Rosenstreich, J H Tu, P R Kinkade, I Maurer-Fogy, J Kahn, R W Barton, P R Farina; A human urine-derived interleukin 1 inhibitor. Homology with deoxyribonuclease I.. J Exp Med 1 November 1988; 168 (5): 1767–1779. doi: https://doi.org/10.1084/jem.168.5.1767
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