Stromal macrophages (M phi) have been localized in situ and isolated within erythroid clusters from human marrow. Stromal M phi arborize in an extensive network uniformly distributed throughout marrow interstitium, and express the phenotype CD4+, CD11a+, CD11c+, CD13+, CD14+, CD16+, CD18+, CD31+, CD32+, FcRI+, HLA-DR+, and CD35-, transferrin receptor-negative, and CD11b (weak). They express endocytic receptor antigens, but show significant differences in myeloid antigen expression compared with freshly harvested or cultured monocytes. Human stromal M phi are therefore specialized mature marrow M phi that are accessible for further investigations in infectious, storage, or hemopoietic disorders.
Isolation and immunocytochemical characterization of human bone marrow stromal macrophages in hemopoietic clusters.
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S H Lee, P R Crocker, S Westaby, N Key, D Y Mason, S Gordon, D J Weatherall; Isolation and immunocytochemical characterization of human bone marrow stromal macrophages in hemopoietic clusters.. J Exp Med 1 September 1988; 168 (3): 1193–1198. doi: https://doi.org/10.1084/jem.168.3.1193
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