Normal human PBMC were analyzed for the presence of cells expressing both T3 and NKH1 antigens, using direct two-color immunofluorescence. In six individuals, NKH1+T3+ cells were found to represent 2.5% of PBMC and 24% of the total number of NKH1+ cells. Purified NKH1+T3+ cells were shown to have the typical morphology of large granular lymphocytes (LGL). NKH1+T3+ cells also exhibited spontaneous cytotoxicity against K562 target cells and this lytic activity could be inhibited by anti-T3 mAb. Similar results were obtained with NKH1+T3+ cells cultured in vitro in lymphocyte-conditioned medium. Taken together, these results indicate that NKH1+T3+ cells represent a unique population of NK-active cells in normal peripheral blood. Although these cells exhibit LGL morphology and NK activity, this appears to be mediated through a functional T cell-like receptor for target antigen.
A subset of natural killer cells in peripheral blood displays a mature T cell phenotype.
R E Schmidt, C Murray, J F Daley, S F Schlossman, J Ritz; A subset of natural killer cells in peripheral blood displays a mature T cell phenotype.. J Exp Med 1 July 1986; 164 (1): 351–356. doi: https://doi.org/10.1084/jem.164.1.351
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