We examined the ability of a set of cloned chicken ovalbumin (cOVA)-specific, Id-restricted, T cell hybridomas to produce interleukin-2 in response to cOVA presented by the Ia+ B cell lymphoma line, A20-2J. Although viable A20-2J cells presented native, denatured, and fragmented cOVA more or less equally well, A20-2J cells that were glutaraldehyde-fixed could present only enzymatically or chemically fragmented cOVA. These results suggest that antigen fragmentation may be both necessary and sufficient to define accessory cell processing of soluble antigens so that they may be recognized in association with I-region molecules by T cells.
Article| August 01 1983
Antigen recognition by H-2-restricted T cells. I. Cell-free antigen processing.
Online Issn: 1540-9538
Print Issn: 0022-1007
J Exp Med (1983) 158 (2): 303–316.
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R Shimonkevitz, J Kappler, P Marrack, H Grey; Antigen recognition by H-2-restricted T cells. I. Cell-free antigen processing.. J Exp Med 1 August 1983; 158 (2): 303–316. doi: https://doi.org/10.1084/jem.158.2.303
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