Of 12 T-lymphoma cell lines investigated, one line, EL-4 azgr (Thy-1+, Lyt-1+, Lyt-2-3-,Ia-, T-200+, sIg-, and FcR-) and, to a lesser extent, the parental cell line, EL-4, produced T cell growth factor(s) (TCGF) when stimulated by the T-cell mitogen concanavalin A (Con A). Induced production of TCGF-E was detected by 6 h and maximal at 18-24 h. Purified TCGF-E from this source had an approximately 30,000 mol wt and the biological activity of TCGF produced by whole spleen cells, including: augmentation of T cell-mitogen responses, cytotoxic T lymphocyte (CTL) proliferation support dependence, augmented generation of CTL, lack of strain specificity, and failure to stimulate resting T cells. TCGF-E is neither synthesized or secreted by this lymphoma cell line unless stimulated by Con A. X-irradiation up to 7,000 rad failed to inhibit synthesis and secretion. These observations have a practical application in providing a relatively homogeneous clonal cell product for T cell culture support and for structural and functional studies of the TCGF molecule(s). They suggest also a model for examining mechanisms of triggering production and secretion of a regulatory molecule that controls T cell functions.
Article| November 01 1980
Mitogen-initiated synthesis and secretion of T cell growth factor(s) by a T-lymphoma cell line.
R T Smith
Online Issn: 1540-9538
Print Issn: 0022-1007
J Exp Med (1980) 152 (5): 1436–1441.
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S Shimizu, Y Konaka, R T Smith; Mitogen-initiated synthesis and secretion of T cell growth factor(s) by a T-lymphoma cell line.. J Exp Med 1 November 1980; 152 (5): 1436–1441. doi: https://doi.org/10.1084/jem.152.5.1436
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