BALB/c mice given allogeneic (C57BL/Ka) bone marrow cells after toal lymphoid irradiation become stable chimeras approximately 80% donor-type and 20% host-type cells in the spleen. The chimeras doe not develop graft vs. host disease (GVHD). Purified cells of C57BL/Ka origin from the chimeras mediated GVHD in lightly irradiated C3H (third party), but not in BALB/c (host-strain) mice. Thus graft vs. host tolerance in the chimeras could not be explained by complete immunodeficiency of donor-type cells, serum blocking factors, or suppressor cells of host (BALB/c) origin. Clonal deletion or suppression of lymphocytes reactive with host tissues remain possible explanations. The transfer of donor-type chimeric spleen cells to BALB/c recipients given 500-550 rad whole-body irradiation WBI led to stable mixed chimerism in approximately 50% of recipients. The cells were presumably acting as tolerogens because similarly irradiated BALB/c mice given (BALB/c X C57BL/Ka)F1 spleen or bone marrow cells also became stable mixed chimeras.
Article| September 01 1980
Cellular basis of graft versus host tolerance in chimeras prepared with total lymphoid irradiation.
H S Kaplan
J Exp Med (1980) 152 (3): 736–741.
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M Gottlieb, S Strober, H S Kaplan; Cellular basis of graft versus host tolerance in chimeras prepared with total lymphoid irradiation.. J Exp Med 1 September 1980; 152 (3): 736–741. doi: https://doi.org/10.1084/jem.152.3.736
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