Protein kinase activity has been detected associated with the outer surface of guinea pig peritoneal macrophages. Macrophages incubated with [gamma-32P]ATP incorporated 32P-phosphate into cell-associated proteins. Inorganic phosphate did not compete, nor could inorganic [32P]phosphate substitute as the phosphate donor, demonstrating that transfer of phosphate from ATP to protein is direct and extracellular. The macrophage-associated protein kinase was also shown to phosphorylate added acceptor protein (histone) and to be tightly associated with the cell surface. Thus, a new ectoenzyme, a protein kinase, has been detected in macrophages.
Article| October 01 1978
Protein kinase activity associated with the surface of guinea pig macrophages.
Online Issn: 1540-9538
Print Issn: 0022-1007
J Exp Med (1978) 148 (4): 1099–1104.
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E Remold-O'Donnell; Protein kinase activity associated with the surface of guinea pig macrophages.. J Exp Med 1 October 1978; 148 (4): 1099–1104. doi: https://doi.org/10.1084/jem.148.4.1099
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