Interactions of CRP with various substrates in the presence of human serum have been shown to result in efficient activation of C components C1-C5. We now report the ability of CRP to initiate C-dependent hemolysis. For this purpose CRP was isolated by affinity chromatography using pneumococcal CPS and gel filtration; its purity was established by several criteria. Erythrocytes were coated with CPS (E-CPS) and passively sensitized with CRP. C-dependent lysis of these cells was observed upon the addition of suitably absorbed human serum, and the efficiency of hemolysis compared favorably with that initiated by rabbit IgG anti-CPS antibody. CRP also sensitized E-CPS for lysis by guinea pig C; partial lysis was seen when C4-deficient guinea pig serum was used, suggesting that CRP also shares with antibody the ability of CRP to fully activate the C system and provide further evidence for a role for CRP similar to that of antibody in the initiation and modulation of inflammatory reactions via the complete system.
Interactions of C-reactive protein with the complement system. III. Complement-dependent passive hemolysis initiated by CRP.
A.P. Osmand, R.F. Mortensen, Joan Siegel, H. Gewurz; Interactions of C-reactive protein with the complement system. III. Complement-dependent passive hemolysis initiated by CRP. . J Exp Med 1 November 1975; 142 (5): 1065–1077. doi: https://doi.org/10.1084/jem.142.5.1065
Download citation file: