The spleen T cells from mice immunized 6 days earlier with either chicken gamma globulin (CGG) or with donkey erythrocytes (DRC) were rosetted with CGG-coated sheep erythrocytes or with DRC. The immune rosettes (RFC) (antigen-binding cells) were separated from the bulk of nonrosette-forming cells (non-RFC) by 1-g velocity sedimentation and the RFC and non-RFC tested for helper activity in cooperative antihapten responses in vitro. RFC or non-RFC were mixed with normal or hapten-primed spleen cells, challenged with the appropriate hapten-carrier conjugate and cultured for 4 days in Marbrook tissue cultures. The helping activity was quantitated from the numbers of antihapten antibody-producing cells generated per culture. The results show that specific helper cell activity could be selectively recovered in the immune rosette-forming cell population whereas the non-RFC population was depleted of help. These findings indicate that the helper T cells express specific antigen binding receptors.
Article| October 01 1975
Antigen-binding T cells as helper cells. Separation of helper cells by immune rosette formation.
L C Andersson
Online Issn: 1540-9538
Print Issn: 0022-1007
J Exp Med (1975) 142 (4): 1035–1039.
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S Kontiainen, L C Andersson; Antigen-binding T cells as helper cells. Separation of helper cells by immune rosette formation.. J Exp Med 1 October 1975; 142 (4): 1035–1039. doi: https://doi.org/10.1084/jem.142.4.1035
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