Mouse thymus cells, educated to poly(tyrosyl,glutamyl)-polyDLalanyl--polylysyl [(T,G)-A--L], release an antigen-specific factor on brief culture in vitro. The factor cooperates with bone marrow cells in the antibody response to (T,G)-A--L in irradiated recipients. Its mol wt determined from Sephadex G100 chromatography is in the region of 50,000. The factor is removed by specific antigen-coated columns, but not by anti-immunoglobulin (anti-Fab, anti-µ, anti-Fv) adsorbents. The factor is removed by alloantisera directed against the H-2 haplotype of the strain in which it is produced. Moreover, only antisera with specificity for the K side of H-2 were successful in removing the factor activity.
ANTIGEN-SPECIFIC T-CELL FACTOR IN CELL COOPERATION: PHYSICAL PROPERTIES AND MAPPING IN THE LEFT-HAND (K) HALF OF H-2
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A. J. Munro, M. J. Taussig, Rosalie Campbell, Heather Williams, Yvonne Lawson; ANTIGEN-SPECIFIC T-CELL FACTOR IN CELL COOPERATION: PHYSICAL PROPERTIES AND MAPPING IN THE LEFT-HAND (K) HALF OF H-2 . J Exp Med 1 December 1974; 140 (6): 1579–1587. doi: https://doi.org/10.1084/jem.140.6.1579
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