In conclusion, it may be pointed out that this is the first time that Treponema pallidum of Schaudinn has been proven beyond all doubt to have been obtained in pure culture. The method of cultivation described would appear also to be suitable for obtaining indefinite generations of the microorganism. Doubtless slight modifications will adapt it to a larger number of strains and possibly to the cultivation of all strains and to still other species of treponema. Finally, it may now be accepted as established that the testicular lesions produced in rabbits by means of syphilitic materials are the result of the multiplication of the pallida and not of some associated indefinite parasite.

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