Interaction of microgram quantities of highly purified rabbit anti-TNP antibodies with TNP-substituted HeLa, HEp-2, and L cells caused an intense stimulation of radioactive nucleoside ([125I]UdR and [3H]TdR) uptake which was maximal 24–72 h after exposure of cells to antibody. The stimulation of nucleoside uptake and presumaly DNA synthesis was shown to be immuno logically mediated because unsubstituted cells were not stimulated by anti-TNP antibody, normal rabbit gamma globulin did not stimulate TNP-cells, and a hapten inhibitor, ϵ-DNP-lysine, prevented the stimulation of TNP-cells by anti-TNP antibody. These findings demonstrate that interaction of antibody with cell surface antigen can alter cell membrane transport, and possibly can enhance cell growth.
HUMORAL IMMUNOSTIMULATION : I. INCREASED UPTAKE OF [125I]IODODEOXYURIDINE AND [3H]THYMIDINE INTO TNP-CELLS TREATED WITH ANTI-TNP ANTIBODY
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William T. Shearer, Gordon W. Philpott, Charles W. Parker; HUMORAL IMMUNOSTIMULATION : I. INCREASED UPTAKE OF [125I]IODODEOXYURIDINE AND [3H]THYMIDINE INTO TNP-CELLS TREATED WITH ANTI-TNP ANTIBODY . J Exp Med 1 February 1974; 139 (2): 367–379. doi: https://doi.org/10.1084/jem.139.2.367
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