IN VITRO STUDIES OF MAMMALIAN SOMATIC CELL VARIATION I. DETECTION OF H-2 PHENOTYPE IN CULTURED MOUSE CELL LINES

Cann , Howard M.; Herzenberg , Leonard A.

doi:10.1084/jem.117.2.259

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Article| February 01 1963

IN VITRO STUDIES OF MAMMALIAN SOMATIC CELL VARIATION : I. DETECTION OF H-2 PHENOTYPE IN CULTURED MOUSE CELL LINES

Howard M. Cann,

Howard M. Cann

From the Department of Genetics, Stanford University School of Medicine, Palo Alto

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Leonard A. Herzenberg

From the Department of Genetics, Stanford University School of Medicine, Palo Alto

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Author and Article Information

Howard M. Cann

From the Department of Genetics, Stanford University School of Medicine, Palo Alto

Leonard A. Herzenberg

From the Department of Genetics, Stanford University School of Medicine, Palo Alto

Received: October 26 1962

Online ISSN: 1540-9538

Print ISSN: 0022-1007

1963

J Exp Med (1963) 117 (2): 259–265.

https://doi.org/10.1084/jem.117.2.259

The isoantigenic phenotype of the H-2 locus has been detected by isohemagglutinin absorption in a line of mouse lymphoma cells growing continuously in culture for 6 years and in two established lines of fibroblastic mouse cells growing continuously in culture for 1 year. Quantitative absorption studies suggest that the concentration of H-2 isoantigens is higher in the cultured lymphoma cells than in the other two fibroblastic cell lines.

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IN VITRO STUDIES OF MAMMALIAN SOMATIC CELL VARIATION : I. DETECTION OF H-2 PHENOTYPE IN CULTURED MOUSE CELL LINES

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